Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101299 |
Resumo: | Poly(ethylene oxide-co-dimethylsiloxane) was thermally immobilized on silica particles-Si(PEO)-to separate small polar compounds with water-rich mobile phases. Poly(ethylene oxide-co-dimethylsiloxane) content on Si(PEO) stationary phase was optimized using a central composite design. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis morphologically and structurally characterized the optimized material. Separation of standard test mixtures showed that the Si(PEO) phase had a typical reversed-phase elution order. However, the Si(PEO) phase retained polar compounds better than C18 or aqueous C18 phases under water-rich mobile phases. Under this condition, small changes in the acetonitrile fraction resulted in a marked increase in the retention of some polar drugs on the Si(PEO) phase. A typical condition observed in per aqueous liquid chromatography separations, a more environmentally friendly liquid chromatography approach. On the other hand, hydrophobic compounds showed lower mass transfer rates due to their low solubility in the aqueous mobile phase. Thus, the Si(PEO) phase was more suitable and efficient for separating polar or hydrophilic compounds. |
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Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary PhaseHPLCstationary phasepoly(ethylene oxide-co-dimethylsiloxane)polar compoundsaqueous mobile phasePoly(ethylene oxide-co-dimethylsiloxane) was thermally immobilized on silica particles-Si(PEO)-to separate small polar compounds with water-rich mobile phases. Poly(ethylene oxide-co-dimethylsiloxane) content on Si(PEO) stationary phase was optimized using a central composite design. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis morphologically and structurally characterized the optimized material. Separation of standard test mixtures showed that the Si(PEO) phase had a typical reversed-phase elution order. However, the Si(PEO) phase retained polar compounds better than C18 or aqueous C18 phases under water-rich mobile phases. Under this condition, small changes in the acetonitrile fraction resulted in a marked increase in the retention of some polar drugs on the Si(PEO) phase. A typical condition observed in per aqueous liquid chromatography separations, a more environmentally friendly liquid chromatography approach. On the other hand, hydrophobic compounds showed lower mass transfer rates due to their low solubility in the aqueous mobile phase. Thus, the Si(PEO) phase was more suitable and efficient for separating polar or hydrophilic compounds.Sociedade Brasileira de Química2022-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101299Journal of the Brazilian Chemical Society v.33 n.11 2022reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20220061info:eu-repo/semantics/openAccessCarvalho,Giselle O.Santos,Allyson L. R.Faria,Anizio M.eng2022-10-21T00:00:00Zoai:scielo:S0103-50532022001101299Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2022-10-21T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
title |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
spellingShingle |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase Carvalho,Giselle O. HPLC stationary phase poly(ethylene oxide-co-dimethylsiloxane) polar compounds aqueous mobile phase |
title_short |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
title_full |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
title_fullStr |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
title_full_unstemmed |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
title_sort |
Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase |
author |
Carvalho,Giselle O. |
author_facet |
Carvalho,Giselle O. Santos,Allyson L. R. Faria,Anizio M. |
author_role |
author |
author2 |
Santos,Allyson L. R. Faria,Anizio M. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Carvalho,Giselle O. Santos,Allyson L. R. Faria,Anizio M. |
dc.subject.por.fl_str_mv |
HPLC stationary phase poly(ethylene oxide-co-dimethylsiloxane) polar compounds aqueous mobile phase |
topic |
HPLC stationary phase poly(ethylene oxide-co-dimethylsiloxane) polar compounds aqueous mobile phase |
description |
Poly(ethylene oxide-co-dimethylsiloxane) was thermally immobilized on silica particles-Si(PEO)-to separate small polar compounds with water-rich mobile phases. Poly(ethylene oxide-co-dimethylsiloxane) content on Si(PEO) stationary phase was optimized using a central composite design. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis morphologically and structurally characterized the optimized material. Separation of standard test mixtures showed that the Si(PEO) phase had a typical reversed-phase elution order. However, the Si(PEO) phase retained polar compounds better than C18 or aqueous C18 phases under water-rich mobile phases. Under this condition, small changes in the acetonitrile fraction resulted in a marked increase in the retention of some polar drugs on the Si(PEO) phase. A typical condition observed in per aqueous liquid chromatography separations, a more environmentally friendly liquid chromatography approach. On the other hand, hydrophobic compounds showed lower mass transfer rates due to their low solubility in the aqueous mobile phase. Thus, the Si(PEO) phase was more suitable and efficient for separating polar or hydrophilic compounds. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101299 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532022001101299 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20220061 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.33 n.11 2022 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318185025896448 |