Vanadium-binding protein in marine plankton from tropical south atlantic ocean
Main Author: | |
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Publication Date: | 2014 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Journal of the Brazilian Chemical Society (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600017 |
Summary: | We investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 µg g-1 (zooplankton), 0.1 to 28.4 µg g-1 (phytoplankton > 64 µm) and < 0.0005 to 49.0 µg g-1 (small phytoplankton > 20 µm). The V speciation in biomolecules was performed by the use of two strategies: (i) coupling of size exclusion chromatography (SEC) for the fractionation of species with inductively coupled plasma mass spectrometry (ICP-MS) and (ii) with size exclusion anionic exchange chromatography with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (SEC-AE-UV-Vis-ICP-MS). The results showed a single fraction containing V associated with a biomolecule in the range of 8 to 16 kDa, with isoeletric points above 8. The preliminary analyses using matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) do not permit to identify such biomolecule, considering the broader size range of the proteins obtained. |
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Vanadium-binding protein in marine plankton from tropical south atlantic oceanbioaccumulationbiogeochemistrychemical ecologyHPLC-ICP-MSmetallothioneinWe investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 µg g-1 (zooplankton), 0.1 to 28.4 µg g-1 (phytoplankton > 64 µm) and < 0.0005 to 49.0 µg g-1 (small phytoplankton > 20 µm). The V speciation in biomolecules was performed by the use of two strategies: (i) coupling of size exclusion chromatography (SEC) for the fractionation of species with inductively coupled plasma mass spectrometry (ICP-MS) and (ii) with size exclusion anionic exchange chromatography with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (SEC-AE-UV-Vis-ICP-MS). The results showed a single fraction containing V associated with a biomolecule in the range of 8 to 16 kDa, with isoeletric points above 8. The preliminary analyses using matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) do not permit to identify such biomolecule, considering the broader size range of the proteins obtained.Sociedade Brasileira de Química2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600017Journal of the Brazilian Chemical Society v.25 n.6 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140088info:eu-repo/semantics/openAccessKütter,Vinicius T.Montes-Bayón,MariaSella,Silvia M.Sanz-Medel,AlfredoSilva-Filho,Emmanoel V.eng2014-07-01T00:00:00Zoai:scielo:S0103-50532014000600017Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-07-01T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
spellingShingle |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean Kütter,Vinicius T. bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein |
title_short |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_full |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_fullStr |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_full_unstemmed |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_sort |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
author |
Kütter,Vinicius T. |
author_facet |
Kütter,Vinicius T. Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. |
author_role |
author |
author2 |
Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Kütter,Vinicius T. Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. |
dc.subject.por.fl_str_mv |
bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein |
topic |
bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein |
description |
We investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 µg g-1 (zooplankton), 0.1 to 28.4 µg g-1 (phytoplankton > 64 µm) and < 0.0005 to 49.0 µg g-1 (small phytoplankton > 20 µm). The V speciation in biomolecules was performed by the use of two strategies: (i) coupling of size exclusion chromatography (SEC) for the fractionation of species with inductively coupled plasma mass spectrometry (ICP-MS) and (ii) with size exclusion anionic exchange chromatography with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (SEC-AE-UV-Vis-ICP-MS). The results showed a single fraction containing V associated with a biomolecule in the range of 8 to 16 kDa, with isoeletric points above 8. The preliminary analyses using matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) do not permit to identify such biomolecule, considering the broader size range of the proteins obtained. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20140088 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.25 n.6 2014 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
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1750318176129777664 |