Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)

Detalhes bibliográficos
Autor(a) principal: Fortes, William Manuel Pereira Antunes
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/14899
Resumo: The occurrence of sedimentary atmospheric particulate matter (MPAS) containing metals and metallic nanoparticles has increased in the world, causing the contamination of aquatic environments, as is the case for Espírito Santo, southeast region of Brazil. MPAS was collected in Vitória’s bay surroundigs, ES, to evaluate the bioaccumulation pattern of metals in Oreochromis niloticus and its possible effects on the gills. The metal profile of MPAS was analyzed and, subsequently, specimens of Nile tilapia, Oreochromis niloticus were exposed to MPAS (1g/L) in a semi-static system, for 30 days, with daily sampling of water for physicochemical parameters analysis and chemical profiling of the dissolved metals. Every 3 days, specimens of tilapia were picked for analysis of bioaccumulation (Total: 11 sample collection) of metals in the viscera and fillet, and every 6 days specimens were picked for histological analysis and determination of the metallothionein concentration in gills (Total: 6 sample collection). For the multi-element analysis, the samples were prepared by acid digestion method and the concentration of metals determined by ICP-MS (Inductively coupled plasma mass spectrometry). The following metals were identified and quantified: B, Al, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Pb, Hg, Ba, Bi, W, Ti, Zr, Y, La, Nb, Ce in the MPAS. Ta has been identified but not quantified. Six metals (Cu, Fe, Al, Zn, Mn, Pb) were chosen/picked for analysis in the fish’s viscera and fillet based on the following criteria: higher concentration in MPAS and limits determined by Brazilian and USA legislation. The concentration of Cu in water was higher than the limits established for freshwater from day 3 to day 15 and on day 24. The concentration of Fe was higher from day 3 to day 12. Al concentration was higher on all days, except on the 18th day. Pb’s concentration only exceeded the limit on the 6th day. The determined bioconcentration factor (BCF) was >1 for all metals analyzed, confirming their bioaccumulation in tilapia. Some metals such as Al, Fe, Mn and Pb showed similar accumulation patterns with significant increase in concentration in the first days until reaching a peak, followed by the subsequent decrease. Cu and Zn showed different bioaccumulation pattern with several peaks and persistence of a high concentration throughout the exposure. There was preferential bioaccumulation of those metals in the viscera compared to the fillet. The analysis of histopathology and the concentration of metallothionein in the gills showed no significant alteration after exposure to MPAS. Exposure to higher concentrations of MPAS may trigger more severe responses.
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spelling Fortes, William Manuel Pereira AntunesFernandes, Marisa Narcisohttp://lattes.cnpq.br/3021357484136942Souza, Iara da Costahttp://lattes.cnpq.br/5391583114634391http://lattes.cnpq.br/72835825694270352021-09-15T11:19:56Z2021-09-15T11:19:56Z2021-02-25FORTES, William Manuel Pereira Antunes. Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus). 2021. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/14899.https://repositorio.ufscar.br/handle/ufscar/14899The occurrence of sedimentary atmospheric particulate matter (MPAS) containing metals and metallic nanoparticles has increased in the world, causing the contamination of aquatic environments, as is the case for Espírito Santo, southeast region of Brazil. MPAS was collected in Vitória’s bay surroundigs, ES, to evaluate the bioaccumulation pattern of metals in Oreochromis niloticus and its possible effects on the gills. The metal profile of MPAS was analyzed and, subsequently, specimens of Nile tilapia, Oreochromis niloticus were exposed to MPAS (1g/L) in a semi-static system, for 30 days, with daily sampling of water for physicochemical parameters analysis and chemical profiling of the dissolved metals. Every 3 days, specimens of tilapia were picked for analysis of bioaccumulation (Total: 11 sample collection) of metals in the viscera and fillet, and every 6 days specimens were picked for histological analysis and determination of the metallothionein concentration in gills (Total: 6 sample collection). For the multi-element analysis, the samples were prepared by acid digestion method and the concentration of metals determined by ICP-MS (Inductively coupled plasma mass spectrometry). The following metals were identified and quantified: B, Al, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Pb, Hg, Ba, Bi, W, Ti, Zr, Y, La, Nb, Ce in the MPAS. Ta has been identified but not quantified. Six metals (Cu, Fe, Al, Zn, Mn, Pb) were chosen/picked for analysis in the fish’s viscera and fillet based on the following criteria: higher concentration in MPAS and limits determined by Brazilian and USA legislation. The concentration of Cu in water was higher than the limits established for freshwater from day 3 to day 15 and on day 24. The concentration of Fe was higher from day 3 to day 12. Al concentration was higher on all days, except on the 18th day. Pb’s concentration only exceeded the limit on the 6th day. The determined bioconcentration factor (BCF) was >1 for all metals analyzed, confirming their bioaccumulation in tilapia. Some metals such as Al, Fe, Mn and Pb showed similar accumulation patterns with significant increase in concentration in the first days until reaching a peak, followed by the subsequent decrease. Cu and Zn showed different bioaccumulation pattern with several peaks and persistence of a high concentration throughout the exposure. There was preferential bioaccumulation of those metals in the viscera compared to the fillet. The analysis of histopathology and the concentration of metallothionein in the gills showed no significant alteration after exposure to MPAS. Exposure to higher concentrations of MPAS may trigger more severe responses.A produção de material particulado atmosférico sedimentável (MPAS) contendo metais e nanopartículas metálicas vem aumentando no mundo, ocasionando a contaminação de ambientes aquáticos, como é o caso do Estado do Espírito Santo, localizado na região Sudeste do Brasil. MPAS foi coletado na região de Vitória, ES, para avaliar o padrão de bioacumulação dos metais em Oreochromis niloticus e seus possíveis efeitos nas brânquias. O perfil químico do MPAS foi analisado e, posteriormente, exemplares de tilápia do Nilo, Oreochromis niloticus, foram expostos ao MPAS (1 g.L-1) em sistema semi-estático, por 30 dias, com coleta diária de amostra de água para análise dos parâmetros físicoquímicos e perfil químico dos metais dissolvidos. A cada 3 dias, espécimes de tilápia foram coletados para análise de bioacumulação (total: 11 coletas) de metais nas vísceras e filé, e a cada 6 dias foram coletados espécimes para análises histológicas e determinação da concentração de metalotioneína das brânquias (Total: 6 coletas). Para a análise multielementar, as amostras foram digeridas e a concentração dos metais determinada por ICP-MS (espectrômetro de massa com plasma indutivamente acoplada). Foram identificados e quantificados os seguintes metais: B, Al, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Pb, Hg, Ba, Bi, W, Ti, Zr, Y, La, Nb, Ce no MPAS. Ta foi identificado, mas não quantificado. Nas visceras e filé dos animais foram avaliados a bioacumulação seis metais (Cu, Fe, Al, Zn, Mn, Pb) com maior concentração no MPAS e limites determinados na legislação brasileira. A concentração de Cu na água foi superior aos limites estabelecidos legislação para águas dulcícolas do dia 3 ao dia 15 e no dia 24. A concentração de Fe foi superior do dia 3 ao dia 12. No caso do Al, a concentração foi superior todos os dias, exceto no 18º. dia e a concentração de Pb só ultrapassou o limite no 6º. dia. O fator de bioconcentração (BCF) foi acima de 1 para todos os metais analisados confirmando a bioacumulação na espécie estudada. Alguns metais como por exemplo Al, Fe, Mn e Pb apresentaram padrões de acumulação semelhantes com aumento significativo na concentração nos primeiros dias até atingir um pico, seguido pela diminuição subsequente. O Cu e Zn apresentaram um padrão de bioacumulação com diversos picos e persistência de uma alta concentração ao longo da exposição. Houve bioacumulação preferencial de metais nos tecidos viscerais quando comparados ao filé. A análise das histopatologias e da concentração de metalotioneína nas brânquias não apresentaram alterações após exposição ao MPAS. Prevê-se que concentrações superiores de MPAS desencadeie respostas mais graves.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPqporUniversidade Federal de São CarlosCâmpus São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBioacumulação de metaisVíscerasFiléHistopatologiaICP-MSMetalotioneínaBrânquiaMetal bioaccumulationGutFilletHistopathologyMetallothioneinGillsCIENCIAS BIOLOGICAS::FISIOLOGIABioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)Metal bioaccumulation in fish (Oreochromis niloticus) exposed to atmospheric particulate material released from steel industries.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissertação de Mestrado.pdfDissertação de Mestrado.pdfDissertação de Mestradoapplication/pdf1682427https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/1/Disserta%c3%a7%c3%a3o%20de%20Mestrado.pdfafe7eb7d31ccb13141eeeac2470f08acMD51Carta comprovante.pdfCarta comprovante.pdfCarta comprovanteapplication/pdf100614https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/2/Carta%20comprovante.pdfc60879dc9390dfb090b044f1aac53b2cMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDissertação de Mestrado.pdf.txtDissertação de Mestrado.pdf.txtExtracted texttext/plain147385https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/4/Disserta%c3%a7%c3%a3o%20de%20Mestrado.pdf.txt2a927d4ff46e26807137de7a8213c611MD54Carta comprovante.pdf.txtCarta comprovante.pdf.txtExtracted texttext/plain1896https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/6/Carta%20comprovante.pdf.txtdb543f3fbdd4d0c6584c7950063201a2MD56THUMBNAILDissertação de Mestrado.pdf.jpgDissertação de Mestrado.pdf.jpgIM Thumbnailimage/jpeg10702https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/5/Disserta%c3%a7%c3%a3o%20de%20Mestrado.pdf.jpgff68375d4b1cbefcd855833c4fea9250MD55Carta comprovante.pdf.jpgCarta comprovante.pdf.jpgIM Thumbnailimage/jpeg6167https://{{ getenv "DSPACE_HOST" "repositorio.ufscar.br" }}/bitstream/ufscar/14899/7/Carta%20comprovante.pdf.jpg426e9467fa11689ddcc8bd68200cbff8MD57ufscar/148992021-09-16 03:40:37.643oai:repositorio.ufscar.br:ufscar/14899Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222021-09-16T03:40:37Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
dc.title.alternative.eng.fl_str_mv Metal bioaccumulation in fish (Oreochromis niloticus) exposed to atmospheric particulate material released from steel industries.
title Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
spellingShingle Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
Fortes, William Manuel Pereira Antunes
Bioacumulação de metais
Vísceras
Filé
Histopatologia
ICP-MS
Metalotioneína
Brânquia
Metal bioaccumulation
Gut
Fillet
Histopathology
Metallothionein
Gills
CIENCIAS BIOLOGICAS::FISIOLOGIA
title_short Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
title_full Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
title_fullStr Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
title_full_unstemmed Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
title_sort Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus)
author Fortes, William Manuel Pereira Antunes
author_facet Fortes, William Manuel Pereira Antunes
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/7283582569427035
dc.contributor.author.fl_str_mv Fortes, William Manuel Pereira Antunes
dc.contributor.advisor1.fl_str_mv Fernandes, Marisa Narciso
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3021357484136942
dc.contributor.advisor-co1.fl_str_mv Souza, Iara da Costa
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/5391583114634391
contributor_str_mv Fernandes, Marisa Narciso
Souza, Iara da Costa
dc.subject.por.fl_str_mv Bioacumulação de metais
Vísceras
Filé
Histopatologia
ICP-MS
Metalotioneína
Brânquia
topic Bioacumulação de metais
Vísceras
Filé
Histopatologia
ICP-MS
Metalotioneína
Brânquia
Metal bioaccumulation
Gut
Fillet
Histopathology
Metallothionein
Gills
CIENCIAS BIOLOGICAS::FISIOLOGIA
dc.subject.eng.fl_str_mv Metal bioaccumulation
Gut
Fillet
Histopathology
Metallothionein
Gills
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::FISIOLOGIA
description The occurrence of sedimentary atmospheric particulate matter (MPAS) containing metals and metallic nanoparticles has increased in the world, causing the contamination of aquatic environments, as is the case for Espírito Santo, southeast region of Brazil. MPAS was collected in Vitória’s bay surroundigs, ES, to evaluate the bioaccumulation pattern of metals in Oreochromis niloticus and its possible effects on the gills. The metal profile of MPAS was analyzed and, subsequently, specimens of Nile tilapia, Oreochromis niloticus were exposed to MPAS (1g/L) in a semi-static system, for 30 days, with daily sampling of water for physicochemical parameters analysis and chemical profiling of the dissolved metals. Every 3 days, specimens of tilapia were picked for analysis of bioaccumulation (Total: 11 sample collection) of metals in the viscera and fillet, and every 6 days specimens were picked for histological analysis and determination of the metallothionein concentration in gills (Total: 6 sample collection). For the multi-element analysis, the samples were prepared by acid digestion method and the concentration of metals determined by ICP-MS (Inductively coupled plasma mass spectrometry). The following metals were identified and quantified: B, Al, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Pb, Hg, Ba, Bi, W, Ti, Zr, Y, La, Nb, Ce in the MPAS. Ta has been identified but not quantified. Six metals (Cu, Fe, Al, Zn, Mn, Pb) were chosen/picked for analysis in the fish’s viscera and fillet based on the following criteria: higher concentration in MPAS and limits determined by Brazilian and USA legislation. The concentration of Cu in water was higher than the limits established for freshwater from day 3 to day 15 and on day 24. The concentration of Fe was higher from day 3 to day 12. Al concentration was higher on all days, except on the 18th day. Pb’s concentration only exceeded the limit on the 6th day. The determined bioconcentration factor (BCF) was >1 for all metals analyzed, confirming their bioaccumulation in tilapia. Some metals such as Al, Fe, Mn and Pb showed similar accumulation patterns with significant increase in concentration in the first days until reaching a peak, followed by the subsequent decrease. Cu and Zn showed different bioaccumulation pattern with several peaks and persistence of a high concentration throughout the exposure. There was preferential bioaccumulation of those metals in the viscera compared to the fillet. The analysis of histopathology and the concentration of metallothionein in the gills showed no significant alteration after exposure to MPAS. Exposure to higher concentrations of MPAS may trigger more severe responses.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-09-15T11:19:56Z
dc.date.available.fl_str_mv 2021-09-15T11:19:56Z
dc.date.issued.fl_str_mv 2021-02-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv FORTES, William Manuel Pereira Antunes. Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus). 2021. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/14899.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/14899
identifier_str_mv FORTES, William Manuel Pereira Antunes. Bioacumulação de metais presentes no material particulado atmosférico sedimentável em tilápia-do-Nilo (Oreochromis niloticus). 2021. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/14899.
url https://repositorio.ufscar.br/handle/ufscar/14899
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
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rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
dc.publisher.program.fl_str_mv Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF
dc.publisher.initials.fl_str_mv UFSCar
publisher.none.fl_str_mv Universidade Federal de São Carlos
Câmpus São Carlos
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