Determination of Six Synthetic Dyes in Sports Drinks by Dispersive Solid-Phase Extraction and HPLC-UV-Vis

• Main Author: Floriano,Luana
• Publication Date: 2018
• Other Authors: Ribeiro,Lucila C., Saibt,Nathália, Bandeira,Nelson M. G., Prestes,Osmar D., Zanella,Renato
• Format: Article
• Language: eng
• Source: Journal of the Brazilian Chemical Society (Online)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018000300602
• topic: food additives; beverages; d-SPE; sample preparation; liquid chromatography

A simple, fast and efficient method was developed for determination of six synthetic dyes (Tartrazine, Sunset Yellow, Amaranth, Ponceau 4R, Indigo Carmine and Brilliant Blue) in sports drinks using dispersive solid-phase extraction (d-SPE) with the polymeric sorbent Oasis® HLB. Sample preparation using d-SPE permitted to simplify the procedure and use less sorbent in comparison with SPE. The use of a syringe for the elution step make this easier and avoid the use of a manifold. High performance liquid chromatography with UV-Vis detection was employed for quantification. Recovery results, evaluated at 0.5; 1.0 and 1.5 mg L-1, were between 76 and 108% with relative standard deviation < 18%. The method limit of quantification was 0.5 mg L-1. The developed method was applied to the analysis of commercial sports drinks and the results indicated that all studied samples presented dye levels in conformity with the Brazilian legislation.