Determination of catechin in green tea using a catechol oxidase biomimetic sensor
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000600024 |
Resumo: | A catechol oxidase biomimetic sensor, based on a novel copper(II) complex, was developed for the determination of catechin in green tea and the results were compared with those obtained by capillary electrophoresis. The dinuclear copper(II) complex, [Cu2(HL)(µ-CH3COO)](ClO4), containing the ligand N,N-[bis-(2-pyridylmethyl)]-N',N'-[(2-hydroxybenzyl)(2-hydroxy-3,5-di-tert-butylbenzyl)]-1,3-propanediamine-2-ol (H3L), was synthesized and characterized by IR, ¹H NMR and elemental analysis. The best conditions for the optimization of the biomimetic sensor were established by square wave voltammetry. The best performance for this sensor was obtained in 75:15:10% (m/m/m) of the graphite powder:nujol:copper(II) complex, 0.05 mol L-1 phosphate buffer solution (pH 7.5) and frequency, pulse amplitude, scan increment at 30 Hz, 80 mV, 3.3 mV, respectively. The analytical curve was linear in the concentration range 4.95 × 10-6 to 3.27 × 10-5 mol L-1 (r = 0.9993) with a detection limit of 2.8 × 10-7 mol L-1. This biomimetic sensor demonstrated long-term stability (9 months; 800 determinations) and reproducibility with a relative standard deviation of 3.5%. The recovery of catechin from green tea samples ranged from 93.8 to 106.9% and the determination, compared with that obtained using capillary electrophoresis, was found to be acceptable at the 95% confidence level. |
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Determination of catechin in green tea using a catechol oxidase biomimetic sensorcatechingreen teabiomimetic sensorsquare wave voltammetryA catechol oxidase biomimetic sensor, based on a novel copper(II) complex, was developed for the determination of catechin in green tea and the results were compared with those obtained by capillary electrophoresis. The dinuclear copper(II) complex, [Cu2(HL)(µ-CH3COO)](ClO4), containing the ligand N,N-[bis-(2-pyridylmethyl)]-N',N'-[(2-hydroxybenzyl)(2-hydroxy-3,5-di-tert-butylbenzyl)]-1,3-propanediamine-2-ol (H3L), was synthesized and characterized by IR, ¹H NMR and elemental analysis. The best conditions for the optimization of the biomimetic sensor were established by square wave voltammetry. The best performance for this sensor was obtained in 75:15:10% (m/m/m) of the graphite powder:nujol:copper(II) complex, 0.05 mol L-1 phosphate buffer solution (pH 7.5) and frequency, pulse amplitude, scan increment at 30 Hz, 80 mV, 3.3 mV, respectively. The analytical curve was linear in the concentration range 4.95 × 10-6 to 3.27 × 10-5 mol L-1 (r = 0.9993) with a detection limit of 2.8 × 10-7 mol L-1. This biomimetic sensor demonstrated long-term stability (9 months; 800 determinations) and reproducibility with a relative standard deviation of 3.5%. The recovery of catechin from green tea samples ranged from 93.8 to 106.9% and the determination, compared with that obtained using capillary electrophoresis, was found to be acceptable at the 95% confidence level.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000600024Journal of the Brazilian Chemical Society v.19 n.6 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000600024info:eu-repo/semantics/openAccessFernandes,Suellen C.Osório,Renata El-Hage M. de BarrosAnjos,Ademir dosNeves,AdemirMicke,Gustavo AmadeuVieira,Iolanda C.eng2008-09-22T00:00:00Zoai:scielo:S0103-50532008000600024Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2008-09-22T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
title |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
spellingShingle |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor Fernandes,Suellen C. catechin green tea biomimetic sensor square wave voltammetry |
title_short |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
title_full |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
title_fullStr |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
title_full_unstemmed |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
title_sort |
Determination of catechin in green tea using a catechol oxidase biomimetic sensor |
author |
Fernandes,Suellen C. |
author_facet |
Fernandes,Suellen C. Osório,Renata El-Hage M. de Barros Anjos,Ademir dos Neves,Ademir Micke,Gustavo Amadeu Vieira,Iolanda C. |
author_role |
author |
author2 |
Osório,Renata El-Hage M. de Barros Anjos,Ademir dos Neves,Ademir Micke,Gustavo Amadeu Vieira,Iolanda C. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes,Suellen C. Osório,Renata El-Hage M. de Barros Anjos,Ademir dos Neves,Ademir Micke,Gustavo Amadeu Vieira,Iolanda C. |
dc.subject.por.fl_str_mv |
catechin green tea biomimetic sensor square wave voltammetry |
topic |
catechin green tea biomimetic sensor square wave voltammetry |
description |
A catechol oxidase biomimetic sensor, based on a novel copper(II) complex, was developed for the determination of catechin in green tea and the results were compared with those obtained by capillary electrophoresis. The dinuclear copper(II) complex, [Cu2(HL)(µ-CH3COO)](ClO4), containing the ligand N,N-[bis-(2-pyridylmethyl)]-N',N'-[(2-hydroxybenzyl)(2-hydroxy-3,5-di-tert-butylbenzyl)]-1,3-propanediamine-2-ol (H3L), was synthesized and characterized by IR, ¹H NMR and elemental analysis. The best conditions for the optimization of the biomimetic sensor were established by square wave voltammetry. The best performance for this sensor was obtained in 75:15:10% (m/m/m) of the graphite powder:nujol:copper(II) complex, 0.05 mol L-1 phosphate buffer solution (pH 7.5) and frequency, pulse amplitude, scan increment at 30 Hz, 80 mV, 3.3 mV, respectively. The analytical curve was linear in the concentration range 4.95 × 10-6 to 3.27 × 10-5 mol L-1 (r = 0.9993) with a detection limit of 2.8 × 10-7 mol L-1. This biomimetic sensor demonstrated long-term stability (9 months; 800 determinations) and reproducibility with a relative standard deviation of 3.5%. The recovery of catechin from green tea samples ranged from 93.8 to 106.9% and the determination, compared with that obtained using capillary electrophoresis, was found to be acceptable at the 95% confidence level. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000600024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000600024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532008000600024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.19 n.6 2008 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318169068666880 |