Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations

Detalhes bibliográficos
Autor(a) principal: Lima,Marcelo B.
Data de Publicação: 2014
Outros Autores: Barreto,Inakã S., Andrade,Stéfani Iury E., Almeida,Luciano F., Araújo,Mário César U.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013
Resumo: The combination of a flow-batch system, quantum dots (QDs) and webcam as detector for the automatic spectrofluorimetric determination of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations is proposed. NAC generates surface interactions that result in enhanced QD fluorescence intensity, which is proportional to analyte concentration. Wavelength selectors are not necessary for excitation and emission process due to the broad excitation spectrum of QDs, the selective and specific reaction between CdTe QDs and NAC, and the mathematical model employed for the treatment of digital images. The limit of detection and relative standard deviation were estimated at 0.14 µg mL-1 and < 1.4% (n = 5), respectively. The accuracy was assessed through recovery test (98.5 to 102.8%).The ruggedness of the method was assessed by comparison of the intra- and inter-day using the iodometric titration method at a 95% confidence level. The system presented satisfactory robustness, high sampling rate (153 h-1), and reduced chemical consumption.
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spelling Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulationsflow-batch systemdigital imagesCdTe quantum dotsfluorescenceN-acetyl-L-cysteinepharmaceutical formulationsThe combination of a flow-batch system, quantum dots (QDs) and webcam as detector for the automatic spectrofluorimetric determination of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations is proposed. NAC generates surface interactions that result in enhanced QD fluorescence intensity, which is proportional to analyte concentration. Wavelength selectors are not necessary for excitation and emission process due to the broad excitation spectrum of QDs, the selective and specific reaction between CdTe QDs and NAC, and the mathematical model employed for the treatment of digital images. The limit of detection and relative standard deviation were estimated at 0.14 µg mL-1 and < 1.4% (n = 5), respectively. The accuracy was assessed through recovery test (98.5 to 102.8%).The ruggedness of the method was assessed by comparison of the intra- and inter-day using the iodometric titration method at a 95% confidence level. The system presented satisfactory robustness, high sampling rate (153 h-1), and reduced chemical consumption.Sociedade Brasileira de Química2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013Journal of the Brazilian Chemical Society v.25 n.9 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140152info:eu-repo/semantics/openAccessLima,Marcelo B.Barreto,Inakã S.Andrade,Stéfani Iury E.Almeida,Luciano F.Araújo,Mário César U.eng2014-09-30T00:00:00Zoai:scielo:S0103-50532014000900013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-09-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
title Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
spellingShingle Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
Lima,Marcelo B.
flow-batch system
digital images
CdTe quantum dots
fluorescence
N-acetyl-L-cysteine
pharmaceutical formulations
title_short Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
title_full Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
title_fullStr Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
title_full_unstemmed Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
title_sort Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
author Lima,Marcelo B.
author_facet Lima,Marcelo B.
Barreto,Inakã S.
Andrade,Stéfani Iury E.
Almeida,Luciano F.
Araújo,Mário César U.
author_role author
author2 Barreto,Inakã S.
Andrade,Stéfani Iury E.
Almeida,Luciano F.
Araújo,Mário César U.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima,Marcelo B.
Barreto,Inakã S.
Andrade,Stéfani Iury E.
Almeida,Luciano F.
Araújo,Mário César U.
dc.subject.por.fl_str_mv flow-batch system
digital images
CdTe quantum dots
fluorescence
N-acetyl-L-cysteine
pharmaceutical formulations
topic flow-batch system
digital images
CdTe quantum dots
fluorescence
N-acetyl-L-cysteine
pharmaceutical formulations
description The combination of a flow-batch system, quantum dots (QDs) and webcam as detector for the automatic spectrofluorimetric determination of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations is proposed. NAC generates surface interactions that result in enhanced QD fluorescence intensity, which is proportional to analyte concentration. Wavelength selectors are not necessary for excitation and emission process due to the broad excitation spectrum of QDs, the selective and specific reaction between CdTe QDs and NAC, and the mathematical model employed for the treatment of digital images. The limit of detection and relative standard deviation were estimated at 0.14 µg mL-1 and < 1.4% (n = 5), respectively. The accuracy was assessed through recovery test (98.5 to 102.8%).The ruggedness of the method was assessed by comparison of the intra- and inter-day using the iodometric titration method at a 95% confidence level. The system presented satisfactory robustness, high sampling rate (153 h-1), and reduced chemical consumption.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20140152
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.25 n.9 2014
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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