Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013 |
Resumo: | The combination of a flow-batch system, quantum dots (QDs) and webcam as detector for the automatic spectrofluorimetric determination of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations is proposed. NAC generates surface interactions that result in enhanced QD fluorescence intensity, which is proportional to analyte concentration. Wavelength selectors are not necessary for excitation and emission process due to the broad excitation spectrum of QDs, the selective and specific reaction between CdTe QDs and NAC, and the mathematical model employed for the treatment of digital images. The limit of detection and relative standard deviation were estimated at 0.14 µg mL-1 and < 1.4% (n = 5), respectively. The accuracy was assessed through recovery test (98.5 to 102.8%).The ruggedness of the method was assessed by comparison of the intra- and inter-day using the iodometric titration method at a 95% confidence level. The system presented satisfactory robustness, high sampling rate (153 h-1), and reduced chemical consumption. |
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Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulationsflow-batch systemdigital imagesCdTe quantum dotsfluorescenceN-acetyl-L-cysteinepharmaceutical formulationsThe combination of a flow-batch system, quantum dots (QDs) and webcam as detector for the automatic spectrofluorimetric determination of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations is proposed. NAC generates surface interactions that result in enhanced QD fluorescence intensity, which is proportional to analyte concentration. Wavelength selectors are not necessary for excitation and emission process due to the broad excitation spectrum of QDs, the selective and specific reaction between CdTe QDs and NAC, and the mathematical model employed for the treatment of digital images. The limit of detection and relative standard deviation were estimated at 0.14 µg mL-1 and < 1.4% (n = 5), respectively. The accuracy was assessed through recovery test (98.5 to 102.8%).The ruggedness of the method was assessed by comparison of the intra- and inter-day using the iodometric titration method at a 95% confidence level. The system presented satisfactory robustness, high sampling rate (153 h-1), and reduced chemical consumption.Sociedade Brasileira de Química2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013Journal of the Brazilian Chemical Society v.25 n.9 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140152info:eu-repo/semantics/openAccessLima,Marcelo B.Barreto,Inakã S.Andrade,Stéfani Iury E.Almeida,Luciano F.Araújo,Mário César U.eng2014-09-30T00:00:00Zoai:scielo:S0103-50532014000900013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-09-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
title |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
spellingShingle |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations Lima,Marcelo B. flow-batch system digital images CdTe quantum dots fluorescence N-acetyl-L-cysteine pharmaceutical formulations |
title_short |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
title_full |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
title_fullStr |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
title_full_unstemmed |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
title_sort |
Using webcam, CdTe quantum dots and flow-batch system for automatic spectrofluorimetric determination of N-Acetyl-L-cysteine in pharmaceutical formulations |
author |
Lima,Marcelo B. |
author_facet |
Lima,Marcelo B. Barreto,Inakã S. Andrade,Stéfani Iury E. Almeida,Luciano F. Araújo,Mário César U. |
author_role |
author |
author2 |
Barreto,Inakã S. Andrade,Stéfani Iury E. Almeida,Luciano F. Araújo,Mário César U. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lima,Marcelo B. Barreto,Inakã S. Andrade,Stéfani Iury E. Almeida,Luciano F. Araújo,Mário César U. |
dc.subject.por.fl_str_mv |
flow-batch system digital images CdTe quantum dots fluorescence N-acetyl-L-cysteine pharmaceutical formulations |
topic |
flow-batch system digital images CdTe quantum dots fluorescence N-acetyl-L-cysteine pharmaceutical formulations |
description |
The combination of a flow-batch system, quantum dots (QDs) and webcam as detector for the automatic spectrofluorimetric determination of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations is proposed. NAC generates surface interactions that result in enhanced QD fluorescence intensity, which is proportional to analyte concentration. Wavelength selectors are not necessary for excitation and emission process due to the broad excitation spectrum of QDs, the selective and specific reaction between CdTe QDs and NAC, and the mathematical model employed for the treatment of digital images. The limit of detection and relative standard deviation were estimated at 0.14 µg mL-1 and < 1.4% (n = 5), respectively. The accuracy was assessed through recovery test (98.5 to 102.8%).The ruggedness of the method was assessed by comparison of the intra- and inter-day using the iodometric titration method at a 95% confidence level. The system presented satisfactory robustness, high sampling rate (153 h-1), and reduced chemical consumption. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20140152 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.25 n.9 2014 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318176223100928 |