Quantification of tryptophan in plasma by high performance liquid chromatography

Detalhes bibliográficos
Autor(a) principal: Pinhati,Renata Romanholi
Data de Publicação: 2012
Outros Autores: Polonini,Hudson Caetano, Brandão,Marcos Antônio Fernandes, Raposo,Nádia Rezende Barbosa, Corchs,Felipe, Gattaz,Wagner Farid
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032
Resumo: A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.
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spelling Quantification of tryptophan in plasma by high performance liquid chromatographytryptophanserotoninHPLCA simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032Química Nova v.35 n.3 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012000300032info:eu-repo/semantics/openAccessPinhati,Renata RomanholiPolonini,Hudson CaetanoBrandão,Marcos Antônio FernandesRaposo,Nádia Rezende BarbosaCorchs,FelipeGattaz,Wagner Farideng2012-04-24T00:00:00Zoai:scielo:S0100-40422012000300032Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2012-04-24T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Quantification of tryptophan in plasma by high performance liquid chromatography
title Quantification of tryptophan in plasma by high performance liquid chromatography
spellingShingle Quantification of tryptophan in plasma by high performance liquid chromatography
Pinhati,Renata Romanholi
tryptophan
serotonin
HPLC
title_short Quantification of tryptophan in plasma by high performance liquid chromatography
title_full Quantification of tryptophan in plasma by high performance liquid chromatography
title_fullStr Quantification of tryptophan in plasma by high performance liquid chromatography
title_full_unstemmed Quantification of tryptophan in plasma by high performance liquid chromatography
title_sort Quantification of tryptophan in plasma by high performance liquid chromatography
author Pinhati,Renata Romanholi
author_facet Pinhati,Renata Romanholi
Polonini,Hudson Caetano
Brandão,Marcos Antônio Fernandes
Raposo,Nádia Rezende Barbosa
Corchs,Felipe
Gattaz,Wagner Farid
author_role author
author2 Polonini,Hudson Caetano
Brandão,Marcos Antônio Fernandes
Raposo,Nádia Rezende Barbosa
Corchs,Felipe
Gattaz,Wagner Farid
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinhati,Renata Romanholi
Polonini,Hudson Caetano
Brandão,Marcos Antônio Fernandes
Raposo,Nádia Rezende Barbosa
Corchs,Felipe
Gattaz,Wagner Farid
dc.subject.por.fl_str_mv tryptophan
serotonin
HPLC
topic tryptophan
serotonin
HPLC
description A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422012000300032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.35 n.3 2012
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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