Metodologia analítica para determinação de folatos e ácido fólico em alimentos
Main Author: | |
---|---|
Publication Date: | 2006 |
Other Authors: | , |
Format: | Article |
Language: | por |
Source: | Química Nova (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500016 |
Summary: | The aim of this work was to develop and to validate a methodology using HPLC for the simultaneous determination of folates and folic acid in foods. The limits of detection and the recovery rates for the vitamins in the certified reference materials were respectively 5 pg/mL and 94-108% for 5-MTHF, 7 pg/mL and 97-102% for THF, 30 pg/mL and 97.9-104% for 5-FTHF, 30 pg/mL and 95-107 for 10-FFA, 5 ng/mL and 97-102% for FA and 5 ng/mL and 98-103% for 10-MFA. Repeatability showed a coefficient of variation below 3.9% for all the vitamins. The proposed methodology was shown to be efficient when applied to different certified reference materials, namely pig's liver (BCR487), powdered milk (BCR421) and a vegetable mixture (BCR485). |
id |
SBQ-3_76870e175e0b3d602b8f332046a44ba2 |
---|---|
oai_identifier_str |
oai:scielo:S0100-40422006000500016 |
network_acronym_str |
SBQ-3 |
network_name_str |
Química Nova (Online) |
repository_id_str |
|
spelling |
Metodologia analítica para determinação de folatos e ácido fólico em alimentosHPLCmethodologyfolatesfoodThe aim of this work was to develop and to validate a methodology using HPLC for the simultaneous determination of folates and folic acid in foods. The limits of detection and the recovery rates for the vitamins in the certified reference materials were respectively 5 pg/mL and 94-108% for 5-MTHF, 7 pg/mL and 97-102% for THF, 30 pg/mL and 97.9-104% for 5-FTHF, 30 pg/mL and 95-107 for 10-FFA, 5 ng/mL and 97-102% for FA and 5 ng/mL and 98-103% for 10-MFA. Repeatability showed a coefficient of variation below 3.9% for all the vitamins. The proposed methodology was shown to be efficient when applied to different certified reference materials, namely pig's liver (BCR487), powdered milk (BCR421) and a vegetable mixture (BCR485).Sociedade Brasileira de Química2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500016Química Nova v.29 n.5 2006reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422006000500016info:eu-repo/semantics/openAccessCatharino,Rodrigo RamosGodoy,Helena TeixeiraLima-Pallone,Juliana Azevedopor2011-09-06T00:00:00Zoai:scielo:S0100-40422006000500016Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2011-09-06T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
title |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
spellingShingle |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos Catharino,Rodrigo Ramos HPLC methodology folates food |
title_short |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
title_full |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
title_fullStr |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
title_full_unstemmed |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
title_sort |
Metodologia analítica para determinação de folatos e ácido fólico em alimentos |
author |
Catharino,Rodrigo Ramos |
author_facet |
Catharino,Rodrigo Ramos Godoy,Helena Teixeira Lima-Pallone,Juliana Azevedo |
author_role |
author |
author2 |
Godoy,Helena Teixeira Lima-Pallone,Juliana Azevedo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Catharino,Rodrigo Ramos Godoy,Helena Teixeira Lima-Pallone,Juliana Azevedo |
dc.subject.por.fl_str_mv |
HPLC methodology folates food |
topic |
HPLC methodology folates food |
description |
The aim of this work was to develop and to validate a methodology using HPLC for the simultaneous determination of folates and folic acid in foods. The limits of detection and the recovery rates for the vitamins in the certified reference materials were respectively 5 pg/mL and 94-108% for 5-MTHF, 7 pg/mL and 97-102% for THF, 30 pg/mL and 97.9-104% for 5-FTHF, 30 pg/mL and 95-107 for 10-FFA, 5 ng/mL and 97-102% for FA and 5 ng/mL and 98-103% for 10-MFA. Repeatability showed a coefficient of variation below 3.9% for all the vitamins. The proposed methodology was shown to be efficient when applied to different certified reference materials, namely pig's liver (BCR487), powdered milk (BCR421) and a vegetable mixture (BCR485). |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500016 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.1590/S0100-40422006000500016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.29 n.5 2006 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318105680150528 |