A STRATEGY FOR THE RAPID IDENTIFICATION OF FUNGAL METABOLITES AND THE DISCOVERY OF THE ANTIVIRAL ACTIVITY OF PYRENOCINE A AND HARZIANOPYRIDONE

• Autor(a) principal: Ióca,Laura P.
• Data de Publicação: 2016
• Outros Autores: Romminger,Stelamar, Santos,Mario F. C., Bandeira,Karin F., Rodrigues,Fabiana T., Kossuga,Miriam H., Nicacio,Karen J., Ferreira,Everton L. F., Morais-Urano,Raquel P., Passos,Messias S., Kohn,Luciana K., Arns,Clarice W., Sette,Lara D., Berlinck,Roberto G. S.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000600720
• topic: fungal metabolites; rapid identification; dereplication; antiviral

The isolation and identification of bioactive metabolites from complex extracts obtained from microbial growth media is a time consuming, costly, and labor-intensive task. A strategy to rapidly identify secondary metabolites isolated from extracts obtained from the culture media of marine-derived and endophytic fungal strains is described. Identification was achieved by HPLC-UV-MS and 1H NMR analyses in combination with data obtained from the Dictionary of Natural Products. Among the compounds identified, (-)-naphthoquinoneimine, citreorosein, emodin, pyrenocine A and harzianopyridone displayed moderate to potent antiviral activity. (-)-Naphthoquinoneimine was isolated as the enantiomer of its previously reported dextrorotatory congener, while 6,7-dihydroxy-2,2-dimethyl-4-chromanone is herein reported for the first time as a natural product.