Estratégias de pré-concentração em eletroforese capilar (CE): parte 1. Manipulação da velocidade eletroforética do analito

• Main Author: Moraes,Maria de Lourdes Leite de
• Publication Date: 2009
• Other Authors: Moraes,Solange Leite de, Pereira,Elisabete Alves, Tavares,Marina Franco Maggi
• Format: Article
• Language: por
• Source: Química Nova (Online)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000400036
• topic: capillary electrophoresis; preconcentration strategies; stacking

Capillary electrophoresis has become a well-established and routine-based separation technique. It is based on the differences between charged analyte mobility in aqueous or organic electrolytes. Its major limitation is the sensitivity due to small sample injection volumes and the narrow diameter of the capillaries, especially when UV detection is used. There are a number of ways to increase the concentration sensitivity. This report shows some on-line preconcentration strategies to perform it in free solution capillary electrophoresis that are based on manipulation of the analyte electrophoretic velocity during the sample introduction (stacking, field amplification and transient isotachophoresis).