Effects of curcumin in an orthotopic murine bladder tumor model

Detalhes bibliográficos
Autor(a) principal: Leite,Katia R. M.
Data de Publicação: 2009
Outros Autores: Chade,Daher C., Sanudo,Adriana, Sakiyama,Bruno Y. P., Batocchio,Gustavo, Srougi,Miguel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: International Braz J Urol (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000500012
Resumo: Cigarette smoking (CS) is the main risk factor for bladder cancer development. There are more than 100 carcinogens present in cigarette smoke. Among the potential mediators of CS-induced alterations is nuclear factor-kappa (NF-κB), which is responsible for the transcription of genes related to cell transformation, tumor promotion, angiogenesis, invasion and metastasis. Curcumin is a polyphenol compound derived from Curcuma longa that suppress cellular transformation, proliferation, invasion, angiogenesis, and metastasis by down regulating NF-κB and its regulated genes. The aim of our study was to assess the effects of curcumin in bladder urothelial carcinoma. We studied the effects of curcumin in vitro and in vivo using the orthotropic syngeneic bladder tumor animal model MB49. Curcumin promotes apoptosis of bladder tumor cells in vitro. In vivo tumors of animals treated with curcumin were significantly smaller as compared to controls. Using immunohistochemistry, we demonstrated a decrease in the expression of Cox-2 by 8% and Cyclin D1 by 13% in the animals treated with curcumin; both genes regulated by NF-κB and related to cell proliferation. In this study, we showed that curcumin acts in bladder urothelial cancer, possibly dowregulating NF-κB-related genes, and could be an option in the treatment of urothelial neoplasms. The results of our study suggest that further research is warranted to confirm our findings.
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spelling Effects of curcumin in an orthotopic murine bladder tumor modelbladder neoplasmsCox-2curcuminCyclin D1NF-κBdrug therapyCigarette smoking (CS) is the main risk factor for bladder cancer development. There are more than 100 carcinogens present in cigarette smoke. Among the potential mediators of CS-induced alterations is nuclear factor-kappa (NF-κB), which is responsible for the transcription of genes related to cell transformation, tumor promotion, angiogenesis, invasion and metastasis. Curcumin is a polyphenol compound derived from Curcuma longa that suppress cellular transformation, proliferation, invasion, angiogenesis, and metastasis by down regulating NF-κB and its regulated genes. The aim of our study was to assess the effects of curcumin in bladder urothelial carcinoma. We studied the effects of curcumin in vitro and in vivo using the orthotropic syngeneic bladder tumor animal model MB49. Curcumin promotes apoptosis of bladder tumor cells in vitro. In vivo tumors of animals treated with curcumin were significantly smaller as compared to controls. Using immunohistochemistry, we demonstrated a decrease in the expression of Cox-2 by 8% and Cyclin D1 by 13% in the animals treated with curcumin; both genes regulated by NF-κB and related to cell proliferation. In this study, we showed that curcumin acts in bladder urothelial cancer, possibly dowregulating NF-κB-related genes, and could be an option in the treatment of urothelial neoplasms. The results of our study suggest that further research is warranted to confirm our findings.Sociedade Brasileira de Urologia2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000500012International braz j urol v.35 n.5 2009reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/S1677-55382009000500012info:eu-repo/semantics/openAccessLeite,Katia R. M.Chade,Daher C.Sanudo,AdrianaSakiyama,Bruno Y. P.Batocchio,GustavoSrougi,Migueleng2009-12-07T00:00:00Zoai:scielo:S1677-55382009000500012Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2009-12-07T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false
dc.title.none.fl_str_mv Effects of curcumin in an orthotopic murine bladder tumor model
title Effects of curcumin in an orthotopic murine bladder tumor model
spellingShingle Effects of curcumin in an orthotopic murine bladder tumor model
Leite,Katia R. M.
bladder neoplasms
Cox-2
curcumin
Cyclin D1
NF-κB
drug therapy
title_short Effects of curcumin in an orthotopic murine bladder tumor model
title_full Effects of curcumin in an orthotopic murine bladder tumor model
title_fullStr Effects of curcumin in an orthotopic murine bladder tumor model
title_full_unstemmed Effects of curcumin in an orthotopic murine bladder tumor model
title_sort Effects of curcumin in an orthotopic murine bladder tumor model
author Leite,Katia R. M.
author_facet Leite,Katia R. M.
Chade,Daher C.
Sanudo,Adriana
Sakiyama,Bruno Y. P.
Batocchio,Gustavo
Srougi,Miguel
author_role author
author2 Chade,Daher C.
Sanudo,Adriana
Sakiyama,Bruno Y. P.
Batocchio,Gustavo
Srougi,Miguel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Leite,Katia R. M.
Chade,Daher C.
Sanudo,Adriana
Sakiyama,Bruno Y. P.
Batocchio,Gustavo
Srougi,Miguel
dc.subject.por.fl_str_mv bladder neoplasms
Cox-2
curcumin
Cyclin D1
NF-κB
drug therapy
topic bladder neoplasms
Cox-2
curcumin
Cyclin D1
NF-κB
drug therapy
description Cigarette smoking (CS) is the main risk factor for bladder cancer development. There are more than 100 carcinogens present in cigarette smoke. Among the potential mediators of CS-induced alterations is nuclear factor-kappa (NF-κB), which is responsible for the transcription of genes related to cell transformation, tumor promotion, angiogenesis, invasion and metastasis. Curcumin is a polyphenol compound derived from Curcuma longa that suppress cellular transformation, proliferation, invasion, angiogenesis, and metastasis by down regulating NF-κB and its regulated genes. The aim of our study was to assess the effects of curcumin in bladder urothelial carcinoma. We studied the effects of curcumin in vitro and in vivo using the orthotropic syngeneic bladder tumor animal model MB49. Curcumin promotes apoptosis of bladder tumor cells in vitro. In vivo tumors of animals treated with curcumin were significantly smaller as compared to controls. Using immunohistochemistry, we demonstrated a decrease in the expression of Cox-2 by 8% and Cyclin D1 by 13% in the animals treated with curcumin; both genes regulated by NF-κB and related to cell proliferation. In this study, we showed that curcumin acts in bladder urothelial cancer, possibly dowregulating NF-κB-related genes, and could be an option in the treatment of urothelial neoplasms. The results of our study suggest that further research is warranted to confirm our findings.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000500012
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/S1677-55382009000500012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Urologia
publisher.none.fl_str_mv Sociedade Brasileira de Urologia
dc.source.none.fl_str_mv International braz j urol v.35 n.5 2009
reponame:International Braz J Urol (Online)
instname:Sociedade Brasileira de Urologia (SBU)
instacron:SBU
instname_str Sociedade Brasileira de Urologia (SBU)
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collection International Braz J Urol (Online)
repository.name.fl_str_mv International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)
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