Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus

Detalhes bibliográficos
Autor(a) principal: Koifman,Leandro
Data de Publicação: 2015
Outros Autores: Ornellas,Paulo, Ornellas,Antonio Augusto, Pereira,Denise de Abreu, Zingali,Benedeta Russolina, Cavalcanti,Silvia Maria Baeta, Afonso,Larissa Alves, Sandim,Vanessa, Alves,Gilda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: International Braz J Urol (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382015000400642
Resumo: ABSTRACTPurpose:The aim of this study was to identify possible protein biomarkers and/or candidates for therapeutic targets in tissues of patients with SCCP, infected by HPV, applying one dimensional electrophoresis (1DE), followed by direct mass spectrometry (MS) analysis.Materials and Methods:Tissues from 10 HPV positive patients with SCCP and from 10 patients with HPV negative non-tumorous penile foreskins were analyzed applying 1D electrophoresis, followed by analysis with direct mass spectrometry (MS).Results:Sixty-three different proteins were identified in the first group and 50 in the second group. Recognition was possible for 28 proteins exclusively detected in Group 1 and 21 proteins presented only in Group 2.Conclusion:Some proteins in the first group are directly involved in the development of other types of cancer, and therefore, suitable for analysis. Complement C3 protein is a strong candidate for evaluating SCCP patients.
id SBU-1_a1f47f940772fbd100a701ad43efefe6
oai_identifier_str oai:scielo:S1677-55382015000400642
network_acronym_str SBU-1
network_name_str International Braz J Urol (Online)
repository_id_str
spelling Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirusCarcinomaSquamous CellPenisHuman papillomavirusABSTRACTPurpose:The aim of this study was to identify possible protein biomarkers and/or candidates for therapeutic targets in tissues of patients with SCCP, infected by HPV, applying one dimensional electrophoresis (1DE), followed by direct mass spectrometry (MS) analysis.Materials and Methods:Tissues from 10 HPV positive patients with SCCP and from 10 patients with HPV negative non-tumorous penile foreskins were analyzed applying 1D electrophoresis, followed by analysis with direct mass spectrometry (MS).Results:Sixty-three different proteins were identified in the first group and 50 in the second group. Recognition was possible for 28 proteins exclusively detected in Group 1 and 21 proteins presented only in Group 2.Conclusion:Some proteins in the first group are directly involved in the development of other types of cancer, and therefore, suitable for analysis. Complement C3 protein is a strong candidate for evaluating SCCP patients.Sociedade Brasileira de Urologia2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382015000400642International braz j urol v.41 n.4 2015reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/S1677-5538.IBJU.2014.0051info:eu-repo/semantics/openAccessKoifman,LeandroOrnellas,PauloOrnellas,Antonio AugustoPereira,Denise de AbreuZingali,Benedeta RussolinaCavalcanti,Silvia Maria BaetaAfonso,Larissa AlvesSandim,VanessaAlves,Gildaeng2015-10-13T00:00:00Zoai:scielo:S1677-55382015000400642Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2015-10-13T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false
dc.title.none.fl_str_mv Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
title Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
spellingShingle Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
Koifman,Leandro
Carcinoma
Squamous Cell
Penis
Human papillomavirus
title_short Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
title_full Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
title_fullStr Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
title_full_unstemmed Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
title_sort Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
author Koifman,Leandro
author_facet Koifman,Leandro
Ornellas,Paulo
Ornellas,Antonio Augusto
Pereira,Denise de Abreu
Zingali,Benedeta Russolina
Cavalcanti,Silvia Maria Baeta
Afonso,Larissa Alves
Sandim,Vanessa
Alves,Gilda
author_role author
author2 Ornellas,Paulo
Ornellas,Antonio Augusto
Pereira,Denise de Abreu
Zingali,Benedeta Russolina
Cavalcanti,Silvia Maria Baeta
Afonso,Larissa Alves
Sandim,Vanessa
Alves,Gilda
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Koifman,Leandro
Ornellas,Paulo
Ornellas,Antonio Augusto
Pereira,Denise de Abreu
Zingali,Benedeta Russolina
Cavalcanti,Silvia Maria Baeta
Afonso,Larissa Alves
Sandim,Vanessa
Alves,Gilda
dc.subject.por.fl_str_mv Carcinoma
Squamous Cell
Penis
Human papillomavirus
topic Carcinoma
Squamous Cell
Penis
Human papillomavirus
description ABSTRACTPurpose:The aim of this study was to identify possible protein biomarkers and/or candidates for therapeutic targets in tissues of patients with SCCP, infected by HPV, applying one dimensional electrophoresis (1DE), followed by direct mass spectrometry (MS) analysis.Materials and Methods:Tissues from 10 HPV positive patients with SCCP and from 10 patients with HPV negative non-tumorous penile foreskins were analyzed applying 1D electrophoresis, followed by analysis with direct mass spectrometry (MS).Results:Sixty-three different proteins were identified in the first group and 50 in the second group. Recognition was possible for 28 proteins exclusively detected in Group 1 and 21 proteins presented only in Group 2.Conclusion:Some proteins in the first group are directly involved in the development of other types of cancer, and therefore, suitable for analysis. Complement C3 protein is a strong candidate for evaluating SCCP patients.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382015000400642
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382015000400642
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1677-5538.IBJU.2014.0051
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Urologia
publisher.none.fl_str_mv Sociedade Brasileira de Urologia
dc.source.none.fl_str_mv International braz j urol v.41 n.4 2015
reponame:International Braz J Urol (Online)
instname:Sociedade Brasileira de Urologia (SBU)
instacron:SBU
instname_str Sociedade Brasileira de Urologia (SBU)
instacron_str SBU
institution SBU
reponame_str International Braz J Urol (Online)
collection International Braz J Urol (Online)
repository.name.fl_str_mv International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)
repository.mail.fl_str_mv ||brazjurol@brazjurol.com.br
_version_ 1750318074158907392