Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies

Detalhes bibliográficos
Autor(a) principal: Borburema, Henrique Douglas dos Santos
Data de Publicação: 2021
Outros Autores: Barbosa, Êmille Natane de Araújo, Miranda, George Emmanuel Cavalcanti de
Tipo de documento: preprint
Idioma: eng
Título da fonte: SciELO Preprints
Texto Completo: https://preprints.scielo.org/index.php/scielo/preprint/view/2383
Resumo: This study aimed to test the effectiveness of physical and chemical procedures for surface decontamination of Bostrychia binderi Harvey. For this, thalli were washed and immersed in chemical solutions (5% liquid detergent, 1% povidone iodine and 0.2% sodium hypochlorite) at different times and combinations. After treatments the thalli were cultivated in laboratory to verify possible negative effects caused by the procedures to alga. Contaminating organisms in the algal culture medium were quantified at the end of cultivation. Washing, spraying, removing of macrofauna individuals with tweezers, thalli immersion in 5% liquid detergent for 60 seconds, after in 0.2% sodium hypochlorite for 2 minutes were effective procedures to eliminate all contaminants analyzed. Thalli immersion in 1% povidone iodine did not affect negatively the thalli growth, whereas immersion in 0.2% sodium hypochlorite for 5 minutes affected. For establishment of Bostrychia’s unialgal cultures we recommend the proposed protocol in this study.
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spelling Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studiesProtocolo de descontaminação da macroalga Bostrychia binderi Harvey (Rhodophyta) para culturas unialgais e estudos laboratoriaiscontaminantesmanguezaisBostrychietummacroalgashipoclorito de sódio 0,2%Bostrychietumcontaminantsmacroalgaemangrove0.2% sodium hypochloriteThis study aimed to test the effectiveness of physical and chemical procedures for surface decontamination of Bostrychia binderi Harvey. For this, thalli were washed and immersed in chemical solutions (5% liquid detergent, 1% povidone iodine and 0.2% sodium hypochlorite) at different times and combinations. After treatments the thalli were cultivated in laboratory to verify possible negative effects caused by the procedures to alga. Contaminating organisms in the algal culture medium were quantified at the end of cultivation. Washing, spraying, removing of macrofauna individuals with tweezers, thalli immersion in 5% liquid detergent for 60 seconds, after in 0.2% sodium hypochlorite for 2 minutes were effective procedures to eliminate all contaminants analyzed. Thalli immersion in 1% povidone iodine did not affect negatively the thalli growth, whereas immersion in 0.2% sodium hypochlorite for 5 minutes affected. For establishment of Bostrychia’s unialgal cultures we recommend the proposed protocol in this study.Este estudo objetivou testar a eficácia de procedimentos físicos e químicos para descontaminação da superfície de Bostrychia binderi Harvey. Para isto, talos foram lavados e imersos em soluções químicas (detergente líquido 5%, iodopovidona 1% e hipoclorito de sódio 0,2%) em diferentes tempos e combinações. Após os tratamentos, os talos foram cultivados para verificar possíveis efeitos negativos causados ​​pelos procedimentos à alga. Contaminantes no meio de cultura dos talos foram quantificados no final do cultivo. Procedimentos de lavagem, remoção física de indivíduos da macrofauna, imersão de talos em detergente líquido 5% por 60 segundos, depois em hipoclorito de sódio 0,2% por 2 minutos, foram eficazes para eliminar os contaminantes analisados. Imersão de talos em iodopovidona 1% não afetou o crescimento dos talos, enquanto que imersão em hipoclorito de sódio 0,2% por 5 minutos afetou. Para estabelecimento de culturas unialgais de Bostrychia recomendamos o protocolo proposto neste estudo.SciELO PreprintsSciELO PreprintsSciELO Preprints2021-05-27info:eu-repo/semantics/preprintinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://preprints.scielo.org/index.php/scielo/preprint/view/238310.1590/2236-8906-58/2020enghttps://preprints.scielo.org/index.php/scielo/article/view/2383/4119Copyright (c) 2021 Henrique Douglas dos Santos Borburema, Êmille Natane de Araújo Barbosa, George Emmanuel Cavalcanti de Mirandahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBorburema, Henrique Douglas dos Santos Barbosa, Êmille Natane de AraújoMiranda, George Emmanuel Cavalcanti de reponame:SciELO Preprintsinstname:SciELOinstacron:SCI2021-05-27T03:32:15Zoai:ops.preprints.scielo.org:preprint/2383Servidor de preprintshttps://preprints.scielo.org/index.php/scieloONGhttps://preprints.scielo.org/index.php/scielo/oaiscielo.submission@scielo.orgopendoar:2021-05-27T03:32:15SciELO Preprints - SciELOfalse
dc.title.none.fl_str_mv Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
Protocolo de descontaminação da macroalga Bostrychia binderi Harvey (Rhodophyta) para culturas unialgais e estudos laboratoriais
title Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
spellingShingle Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
Borburema, Henrique Douglas dos Santos
contaminantes
manguezais
Bostrychietum
macroalgas
hipoclorito de sódio 0,2%
Bostrychietum
contaminants
macroalgae
mangrove
0.2% sodium hypochlorite
title_short Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
title_full Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
title_fullStr Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
title_full_unstemmed Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
title_sort Decontamination protocol of the macroalga Bostrychia binderi Harvey (Rhodophyta) for unialgal cultures and laboratory studies
author Borburema, Henrique Douglas dos Santos
author_facet Borburema, Henrique Douglas dos Santos
Barbosa, Êmille Natane de Araújo
Miranda, George Emmanuel Cavalcanti de
author_role author
author2 Barbosa, Êmille Natane de Araújo
Miranda, George Emmanuel Cavalcanti de
author2_role author
author
dc.contributor.author.fl_str_mv Borburema, Henrique Douglas dos Santos
Barbosa, Êmille Natane de Araújo
Miranda, George Emmanuel Cavalcanti de
dc.subject.por.fl_str_mv contaminantes
manguezais
Bostrychietum
macroalgas
hipoclorito de sódio 0,2%
Bostrychietum
contaminants
macroalgae
mangrove
0.2% sodium hypochlorite
topic contaminantes
manguezais
Bostrychietum
macroalgas
hipoclorito de sódio 0,2%
Bostrychietum
contaminants
macroalgae
mangrove
0.2% sodium hypochlorite
description This study aimed to test the effectiveness of physical and chemical procedures for surface decontamination of Bostrychia binderi Harvey. For this, thalli were washed and immersed in chemical solutions (5% liquid detergent, 1% povidone iodine and 0.2% sodium hypochlorite) at different times and combinations. After treatments the thalli were cultivated in laboratory to verify possible negative effects caused by the procedures to alga. Contaminating organisms in the algal culture medium were quantified at the end of cultivation. Washing, spraying, removing of macrofauna individuals with tweezers, thalli immersion in 5% liquid detergent for 60 seconds, after in 0.2% sodium hypochlorite for 2 minutes were effective procedures to eliminate all contaminants analyzed. Thalli immersion in 1% povidone iodine did not affect negatively the thalli growth, whereas immersion in 0.2% sodium hypochlorite for 5 minutes affected. For establishment of Bostrychia’s unialgal cultures we recommend the proposed protocol in this study.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-27
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info:eu-repo/semantics/publishedVersion
format preprint
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dc.identifier.uri.fl_str_mv https://preprints.scielo.org/index.php/scielo/preprint/view/2383
10.1590/2236-8906-58/2020
url https://preprints.scielo.org/index.php/scielo/preprint/view/2383
identifier_str_mv 10.1590/2236-8906-58/2020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://preprints.scielo.org/index.php/scielo/article/view/2383/4119
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rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
publisher.none.fl_str_mv SciELO Preprints
SciELO Preprints
SciELO Preprints
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