A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Árvore (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622020000100210 |
Resumo: | ABSTRACT Ergosterol is responsible for important functions in the fungal plasma membrane. The influence of fungitoxic agents on membrane ergosterol content is one of the most important mechanisms of antifungal action and its knowledge allows the generation of products that associate active compounds of different mechanisms, consequently improving the effectiveness of wood preservatives. Therefore, this study optimized a method for quantifying ergosterol in wood-decay fungi. The white-rot species selected were Ganoderma applanatum and Trametes versicolor, while the brown-rot were Gloeophyllum trabeum and Lentinus lepideus. Mycelial discs of each species were transferred to Petri dishes containing a cellophane-covered potato-dextrose-agar medium. Mycelia of each fungus were collected, weighed, and transferred to test tubes with 5 mL of 25% alcoholic potassium hydroxide. The tubes were vortexed for 5 min, subjected to ultrasound for 5 min, incubated at 85 °C for 4 h, followed by the addition of 2 mL of sterile distilled water and 5 mL of n-heptane and subsequent ultrasound shaking for 2 min. The n-heptane layer was analyzed by UV spectrophotometry between 230 and 300 ηm. The blank sample only contained n-heptane. The mycelia wet weight of the fungi ranged from 0.061 to 0.296 g. Ergosterol content was 0.007% for Lentinus lepideus and 0.004% for the other species. The absorbance was higher than the ones observed in the blank for all samples. The adapted method was efficient for ergosterol extraction. |
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A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGISterolMyceliaExtractionABSTRACT Ergosterol is responsible for important functions in the fungal plasma membrane. The influence of fungitoxic agents on membrane ergosterol content is one of the most important mechanisms of antifungal action and its knowledge allows the generation of products that associate active compounds of different mechanisms, consequently improving the effectiveness of wood preservatives. Therefore, this study optimized a method for quantifying ergosterol in wood-decay fungi. The white-rot species selected were Ganoderma applanatum and Trametes versicolor, while the brown-rot were Gloeophyllum trabeum and Lentinus lepideus. Mycelial discs of each species were transferred to Petri dishes containing a cellophane-covered potato-dextrose-agar medium. Mycelia of each fungus were collected, weighed, and transferred to test tubes with 5 mL of 25% alcoholic potassium hydroxide. The tubes were vortexed for 5 min, subjected to ultrasound for 5 min, incubated at 85 °C for 4 h, followed by the addition of 2 mL of sterile distilled water and 5 mL of n-heptane and subsequent ultrasound shaking for 2 min. The n-heptane layer was analyzed by UV spectrophotometry between 230 and 300 ηm. The blank sample only contained n-heptane. The mycelia wet weight of the fungi ranged from 0.061 to 0.296 g. Ergosterol content was 0.007% for Lentinus lepideus and 0.004% for the other species. The absorbance was higher than the ones observed in the blank for all samples. The adapted method was efficient for ergosterol extraction.Sociedade de Investigações Florestais2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622020000100210Revista Árvore v.44 2020reponame:Revista Árvore (Online)instname:Universidade Federal de Viçosa (UFV)instacron:SIF10.1590/1806-908820200000010info:eu-repo/semantics/openAccessPinheiro,Carlos GarridoBianchini,Nadia HelenaPavlack,Alana SilveiraMuniz,Marlove Fátima BriãoBarboza,Victor Dos SantosPavanato,Maria AmáliaGouveia,Fernando NunesHeinzmann,Berta Mariaeng2020-08-05T00:00:00Zoai:scielo:S0100-67622020000100210Revistahttp://www.scielo.br/revistas/rarv/iaboutj.htmPUBhttps://old.scielo.br/oai/scielo-oai.php||r.arvore@ufv.br1806-90880100-6762opendoar:2020-08-05T00:00Revista Árvore (Online) - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
title |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
spellingShingle |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI Pinheiro,Carlos Garrido Sterol Mycelia Extraction |
title_short |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
title_full |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
title_fullStr |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
title_full_unstemmed |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
title_sort |
A METHOD FOR EVALUATING ERGOSTEROL CONTENT IN WOOD-DECAY FUNGI |
author |
Pinheiro,Carlos Garrido |
author_facet |
Pinheiro,Carlos Garrido Bianchini,Nadia Helena Pavlack,Alana Silveira Muniz,Marlove Fátima Brião Barboza,Victor Dos Santos Pavanato,Maria Amália Gouveia,Fernando Nunes Heinzmann,Berta Maria |
author_role |
author |
author2 |
Bianchini,Nadia Helena Pavlack,Alana Silveira Muniz,Marlove Fátima Brião Barboza,Victor Dos Santos Pavanato,Maria Amália Gouveia,Fernando Nunes Heinzmann,Berta Maria |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pinheiro,Carlos Garrido Bianchini,Nadia Helena Pavlack,Alana Silveira Muniz,Marlove Fátima Brião Barboza,Victor Dos Santos Pavanato,Maria Amália Gouveia,Fernando Nunes Heinzmann,Berta Maria |
dc.subject.por.fl_str_mv |
Sterol Mycelia Extraction |
topic |
Sterol Mycelia Extraction |
description |
ABSTRACT Ergosterol is responsible for important functions in the fungal plasma membrane. The influence of fungitoxic agents on membrane ergosterol content is one of the most important mechanisms of antifungal action and its knowledge allows the generation of products that associate active compounds of different mechanisms, consequently improving the effectiveness of wood preservatives. Therefore, this study optimized a method for quantifying ergosterol in wood-decay fungi. The white-rot species selected were Ganoderma applanatum and Trametes versicolor, while the brown-rot were Gloeophyllum trabeum and Lentinus lepideus. Mycelial discs of each species were transferred to Petri dishes containing a cellophane-covered potato-dextrose-agar medium. Mycelia of each fungus were collected, weighed, and transferred to test tubes with 5 mL of 25% alcoholic potassium hydroxide. The tubes were vortexed for 5 min, subjected to ultrasound for 5 min, incubated at 85 °C for 4 h, followed by the addition of 2 mL of sterile distilled water and 5 mL of n-heptane and subsequent ultrasound shaking for 2 min. The n-heptane layer was analyzed by UV spectrophotometry between 230 and 300 ηm. The blank sample only contained n-heptane. The mycelia wet weight of the fungi ranged from 0.061 to 0.296 g. Ergosterol content was 0.007% for Lentinus lepideus and 0.004% for the other species. The absorbance was higher than the ones observed in the blank for all samples. The adapted method was efficient for ergosterol extraction. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622020000100210 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622020000100210 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-908820200000010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
dc.source.none.fl_str_mv |
Revista Árvore v.44 2020 reponame:Revista Árvore (Online) instname:Universidade Federal de Viçosa (UFV) instacron:SIF |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
SIF |
institution |
SIF |
reponame_str |
Revista Árvore (Online) |
collection |
Revista Árvore (Online) |
repository.name.fl_str_mv |
Revista Árvore (Online) - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
||r.arvore@ufv.br |
_version_ |
1750318003455524864 |