Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100329 |
Resumo: | Abstract Cancer is the leading cause of death. Melanoma skin cancer originates in melanocytes and represents 80% of the deaths associated with skin cancer. Vinblastine (VIN) is a chemotherapeutic agent used in the treatment of cancer through disrupting mitotic spindle and tumor development. Curcumin (CUR), a compound extracted from the rhizomes of the Curcuma longa plant, has beneficial effects in preventing the development and progression of cancer while modulating the immune response and oxidative stress. The expression of purinergic receptors, ecto-enzymes, and adenosine can modulate the inflammatory responses in cancer. The activity of enzymes, the markers of cell damage in oxidative stress, the generation of reactive oxygen species (ROS), and the activities of ecto-enzymes in the melanoma cell line were investigated. The human melanoma cell line was treated with curcumin (40 µM), vinblastine (VIN) (20 nM), or a combination of both for 24h. Oxidative stress enzymes and byproducts were measured and compared against the activity of ecto-enzymes. There was a marked increase in ROS production in all groups, but an increase in protein carbonylation was only detected in the VIN group. CUR had an inhibitory effect on extracellular ADP hydrolysis, as evidenced by a significant decrease in ADP substrate removal. VIN possibly increased adenosine formation, as demonstrated by an increase in ADP substrate removal. VIN (alone or in combination with CUR) reduced the activity of ADA, thus increasing the concentration of adenosine in the tumor microenvironment. CUR increased ROS generation in melanoma cells and disrupted the purinergic signaling cascade. Therefore, it may be a promising adjuvant therapy for melanoma, a cancer with a high incidence and lethality. |
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Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cellsmelanomacurcuminskinenzymesadjuvanttreatment.Abstract Cancer is the leading cause of death. Melanoma skin cancer originates in melanocytes and represents 80% of the deaths associated with skin cancer. Vinblastine (VIN) is a chemotherapeutic agent used in the treatment of cancer through disrupting mitotic spindle and tumor development. Curcumin (CUR), a compound extracted from the rhizomes of the Curcuma longa plant, has beneficial effects in preventing the development and progression of cancer while modulating the immune response and oxidative stress. The expression of purinergic receptors, ecto-enzymes, and adenosine can modulate the inflammatory responses in cancer. The activity of enzymes, the markers of cell damage in oxidative stress, the generation of reactive oxygen species (ROS), and the activities of ecto-enzymes in the melanoma cell line were investigated. The human melanoma cell line was treated with curcumin (40 µM), vinblastine (VIN) (20 nM), or a combination of both for 24h. Oxidative stress enzymes and byproducts were measured and compared against the activity of ecto-enzymes. There was a marked increase in ROS production in all groups, but an increase in protein carbonylation was only detected in the VIN group. CUR had an inhibitory effect on extracellular ADP hydrolysis, as evidenced by a significant decrease in ADP substrate removal. VIN possibly increased adenosine formation, as demonstrated by an increase in ADP substrate removal. VIN (alone or in combination with CUR) reduced the activity of ADA, thus increasing the concentration of adenosine in the tumor microenvironment. CUR increased ROS generation in melanoma cells and disrupted the purinergic signaling cascade. Therefore, it may be a promising adjuvant therapy for melanoma, a cancer with a high incidence and lethality.Instituto de Tecnologia do Paraná - Tecpar2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100329Brazilian Archives of Biology and Technology v.65 2022reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2022220187info:eu-repo/semantics/openAccessLunkes,Vinícius LeobetPalma,Taís VidalAssmann,Charles EliasMostardeiro,Vitor BastianelloSchetinger,Maria Rosa ChitolinaMorsch,Vera Maria MelchiorsAndrade,Cinthia Melazzo deeng2022-07-20T00:00:00Zoai:scielo:S1516-89132022000100329Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2022-07-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
title |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
spellingShingle |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells Lunkes,Vinícius Leobet melanoma curcumin skin enzymes adjuvant treatment. |
title_short |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
title_full |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
title_fullStr |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
title_full_unstemmed |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
title_sort |
Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells |
author |
Lunkes,Vinícius Leobet |
author_facet |
Lunkes,Vinícius Leobet Palma,Taís Vidal Assmann,Charles Elias Mostardeiro,Vitor Bastianello Schetinger,Maria Rosa Chitolina Morsch,Vera Maria Melchiors Andrade,Cinthia Melazzo de |
author_role |
author |
author2 |
Palma,Taís Vidal Assmann,Charles Elias Mostardeiro,Vitor Bastianello Schetinger,Maria Rosa Chitolina Morsch,Vera Maria Melchiors Andrade,Cinthia Melazzo de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Lunkes,Vinícius Leobet Palma,Taís Vidal Assmann,Charles Elias Mostardeiro,Vitor Bastianello Schetinger,Maria Rosa Chitolina Morsch,Vera Maria Melchiors Andrade,Cinthia Melazzo de |
dc.subject.por.fl_str_mv |
melanoma curcumin skin enzymes adjuvant treatment. |
topic |
melanoma curcumin skin enzymes adjuvant treatment. |
description |
Abstract Cancer is the leading cause of death. Melanoma skin cancer originates in melanocytes and represents 80% of the deaths associated with skin cancer. Vinblastine (VIN) is a chemotherapeutic agent used in the treatment of cancer through disrupting mitotic spindle and tumor development. Curcumin (CUR), a compound extracted from the rhizomes of the Curcuma longa plant, has beneficial effects in preventing the development and progression of cancer while modulating the immune response and oxidative stress. The expression of purinergic receptors, ecto-enzymes, and adenosine can modulate the inflammatory responses in cancer. The activity of enzymes, the markers of cell damage in oxidative stress, the generation of reactive oxygen species (ROS), and the activities of ecto-enzymes in the melanoma cell line were investigated. The human melanoma cell line was treated with curcumin (40 µM), vinblastine (VIN) (20 nM), or a combination of both for 24h. Oxidative stress enzymes and byproducts were measured and compared against the activity of ecto-enzymes. There was a marked increase in ROS production in all groups, but an increase in protein carbonylation was only detected in the VIN group. CUR had an inhibitory effect on extracellular ADP hydrolysis, as evidenced by a significant decrease in ADP substrate removal. VIN possibly increased adenosine formation, as demonstrated by an increase in ADP substrate removal. VIN (alone or in combination with CUR) reduced the activity of ADA, thus increasing the concentration of adenosine in the tumor microenvironment. CUR increased ROS generation in melanoma cells and disrupted the purinergic signaling cascade. Therefore, it may be a promising adjuvant therapy for melanoma, a cancer with a high incidence and lethality. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100329 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2022220187 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.65 2022 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
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1750318281388982272 |