Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613 |
Resumo: | The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 - phenanthroline and sodium potassium tartarate. Validation of the developed biosensors was carried out with spiked milk samples. |
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Brazilian Archives of Biology and Technology |
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Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in MilkBiosensorUreasehydrosol- gel immobilizationleadmilkmasking agentThe aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 - phenanthroline and sodium potassium tartarate. Validation of the developed biosensors was carried out with spiked milk samples.Instituto de Tecnologia do Paraná - Tecpar2014-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613Brazilian Archives of Biology and Technology v.57 n.4 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201402160info:eu-repo/semantics/openAccessKaur,HardeepKumar,SachinVerma,Neelameng2015-09-03T00:00:00Zoai:scielo:S1516-89132014000400613Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-09-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
title |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
spellingShingle |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk Kaur,Hardeep Biosensor Urease hydrosol- gel immobilization lead milk masking agent |
title_short |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
title_full |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
title_fullStr |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
title_full_unstemmed |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
title_sort |
Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk |
author |
Kaur,Hardeep |
author_facet |
Kaur,Hardeep Kumar,Sachin Verma,Neelam |
author_role |
author |
author2 |
Kumar,Sachin Verma,Neelam |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Kaur,Hardeep Kumar,Sachin Verma,Neelam |
dc.subject.por.fl_str_mv |
Biosensor Urease hydrosol- gel immobilization lead milk masking agent |
topic |
Biosensor Urease hydrosol- gel immobilization lead milk masking agent |
description |
The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 - phenanthroline and sodium potassium tartarate. Validation of the developed biosensors was carried out with spiked milk samples. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-8913201402160 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.4 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276437606400 |