Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method

Detalhes bibliográficos
Autor(a) principal: Parizzi,Silvânia Quintão Fontes
Data de Publicação: 2004
Outros Autores: Andrade,Nélio José de, Silva,Cleuber Antonio de Sá, Soares,Nilda de Fátima Ferreira, Silva,Eldo Antônio Monteiro da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011
Resumo: The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.
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spelling Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count methodBacterial adherenceListeria innocua and Staphylococcus aureusstainless steelpolycarbonate and polypropyleneThe adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.Instituto de Tecnologia do Paraná - Tecpar2004-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011Brazilian Archives of Biology and Technology v.47 n.1 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000100011info:eu-repo/semantics/openAccessParizzi,Silvânia Quintão FontesAndrade,Nélio José deSilva,Cleuber Antonio de SáSoares,Nilda de Fátima FerreiraSilva,Eldo Antônio Monteiro daeng2004-07-02T00:00:00Zoai:scielo:S1516-89132004000100011Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-07-02T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
title Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
spellingShingle Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
Parizzi,Silvânia Quintão Fontes
Bacterial adherence
Listeria innocua and Staphylococcus aureus
stainless steel
polycarbonate and polypropylene
title_short Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
title_full Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
title_fullStr Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
title_full_unstemmed Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
title_sort Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
author Parizzi,Silvânia Quintão Fontes
author_facet Parizzi,Silvânia Quintão Fontes
Andrade,Nélio José de
Silva,Cleuber Antonio de Sá
Soares,Nilda de Fátima Ferreira
Silva,Eldo Antônio Monteiro da
author_role author
author2 Andrade,Nélio José de
Silva,Cleuber Antonio de Sá
Soares,Nilda de Fátima Ferreira
Silva,Eldo Antônio Monteiro da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Parizzi,Silvânia Quintão Fontes
Andrade,Nélio José de
Silva,Cleuber Antonio de Sá
Soares,Nilda de Fátima Ferreira
Silva,Eldo Antônio Monteiro da
dc.subject.por.fl_str_mv Bacterial adherence
Listeria innocua and Staphylococcus aureus
stainless steel
polycarbonate and polypropylene
topic Bacterial adherence
Listeria innocua and Staphylococcus aureus
stainless steel
polycarbonate and polypropylene
description The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.
publishDate 2004
dc.date.none.fl_str_mv 2004-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132004000100011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.47 n.1 2004
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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