Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil
Main Author: | |
---|---|
Publication Date: | 2011 |
Other Authors: | |
Format: | Article |
Language: | eng |
Source: | Brazilian Archives of Biology and Technology |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002 |
Summary: | In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively. |
id |
TECPAR-1_de5b22d2ed0010b16ef2c30b0922ad1e |
---|---|
oai_identifier_str |
oai:scielo:S1516-89132011000400002 |
network_acronym_str |
TECPAR-1 |
network_name_str |
Brazilian Archives of Biology and Technology |
repository_id_str |
|
spelling |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soilhydrolysisstarchfungal growthamylase synthesisIn this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively.Instituto de Tecnologia do Paraná - Tecpar2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002Brazilian Archives of Biology and Technology v.54 n.4 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000400002info:eu-repo/semantics/openAccessNwagu,Tochukwu NwamakaOkolo,Bartholomew Ndubuisieng2011-08-11T00:00:00Zoai:scielo:S1516-89132011000400002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-08-11T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
title |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
spellingShingle |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil Nwagu,Tochukwu Nwamaka hydrolysis starch fungal growth amylase synthesis |
title_short |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
title_full |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
title_fullStr |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
title_full_unstemmed |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
title_sort |
Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil |
author |
Nwagu,Tochukwu Nwamaka |
author_facet |
Nwagu,Tochukwu Nwamaka Okolo,Bartholomew Ndubuisi |
author_role |
author |
author2 |
Okolo,Bartholomew Ndubuisi |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Nwagu,Tochukwu Nwamaka Okolo,Bartholomew Ndubuisi |
dc.subject.por.fl_str_mv |
hydrolysis starch fungal growth amylase synthesis |
topic |
hydrolysis starch fungal growth amylase synthesis |
description |
In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132011000400002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.54 n.4 2011 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318274778759168 |