Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG

Detalhes bibliográficos
Autor(a) principal: Penha,Tânia Regina
Data de Publicação: 2010
Outros Autores: Krüger,Ernesto Renato, Thomaz-Soccol,Vanete, Agottani,Jorge Victor Bacila, Itano,Flávio Hiroshi, Troiano,Ludmilla Della Coletta, Brodzinski,Josiane
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014
Resumo: The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.
id TECPAR-1_f6cca00426f1bfab3ed6604059f1958a
oai_identifier_str oai:scielo:S1516-89132010000100014
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgGMonoclonal antibodiesbovine immunoglobulin GFragment FcThe aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.Instituto de Tecnologia do Paraná - Tecpar2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014Brazilian Archives of Biology and Technology v.53 n.1 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000100014info:eu-repo/semantics/openAccessPenha,Tânia ReginaKrüger,Ernesto RenatoThomaz-Soccol,VaneteAgottani,Jorge Victor BacilaItano,Flávio HiroshiTroiano,Ludmilla Della ColettaBrodzinski,Josianeeng2010-02-25T00:00:00Zoai:scielo:S1516-89132010000100014Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-02-25T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
title Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
spellingShingle Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
Penha,Tânia Regina
Monoclonal antibodies
bovine immunoglobulin G
Fragment Fc
title_short Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
title_full Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
title_fullStr Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
title_full_unstemmed Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
title_sort Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG
author Penha,Tânia Regina
author_facet Penha,Tânia Regina
Krüger,Ernesto Renato
Thomaz-Soccol,Vanete
Agottani,Jorge Victor Bacila
Itano,Flávio Hiroshi
Troiano,Ludmilla Della Coletta
Brodzinski,Josiane
author_role author
author2 Krüger,Ernesto Renato
Thomaz-Soccol,Vanete
Agottani,Jorge Victor Bacila
Itano,Flávio Hiroshi
Troiano,Ludmilla Della Coletta
Brodzinski,Josiane
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Penha,Tânia Regina
Krüger,Ernesto Renato
Thomaz-Soccol,Vanete
Agottani,Jorge Victor Bacila
Itano,Flávio Hiroshi
Troiano,Ludmilla Della Coletta
Brodzinski,Josiane
dc.subject.por.fl_str_mv Monoclonal antibodies
bovine immunoglobulin G
Fragment Fc
topic Monoclonal antibodies
bovine immunoglobulin G
Fragment Fc
description The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000100014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.1 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318273610645504

Registros relacionados