Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/672 https://repositorio.ucb.br:9443/jspui/handle/123456789/7923 |
Resumo: | Canatoxin, a urease isoform from Canavalia ensiformis seeds, shows insecticidal activity against different insect species. Its toxicity relies on an internal 10 kDa peptide (pepcanatox), released by hydrolysis of Canatoxin by cathepsins in the digestive system of susceptible insects. In the present work, based on the N-terminal sequence of pepcanatox, we have designed primers to amplify by PCR a 270-bp fragment corresponding to pepcanatox using JBURE-II cDNA (one of the urease isoforms cloned from C. ensiformis, with high identity to JBURE-I, the classical urease) as a template. This amplicon named jaburetox-2 was cloned into pET 101 vector to obtain heterologous expression in Escherichia coli of the recombinant protein in C-terminal fusion with V-5 epitope and 6-His tag. Jaburetox-2Ec was purified on Nickel-NTA resin and bioassayed in insect models. Dysdercus peruvianus larvae were fed on cotton seed meal diets containing 0.01% (w/w) Jaburetox-2Ec and, after 11 days, all individuals were dead. Jaburetox-2Ec was also tested against Spodoptera frugiperda larvae and caused 100% mortality. In contrast, high doses of Jaburetox-2Ec were innocuous when injected or ingested by mice and neonate rats. Modeling of Jaburetox-2Ec, in comparison with other peptide structures, revealed a prominent b-hairpin motif consistent with an insecticidal activity based on either neurotoxicity or cell permeation. |
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Mulinari, Fernanda FontanaStanisçuaski, FernandaBertholdo-Vargas, Lúcia R.Postal, MelissaOliveira-Neto, Osmundo Brilhante deRigden, Daniel JonhGrossi-de-Sá, Maria de FátimaCarlini, Celia Regina2016-10-10T03:53:07Z2016-10-10T03:53:07Z2007-08MULINARI, Fernanda Fontana, et al. Jaburetox-2Ec: an insecticidal peptide derived from an: isoform of urease from the plant Canavalia ensiformis. Peptides, v. 28, p. 2042- 2050, 2007.http://twingo.ucb.br:8080/jspui/handle/10869/672https://repositorio.ucb.br:9443/jspui/handle/123456789/7923Canatoxin, a urease isoform from Canavalia ensiformis seeds, shows insecticidal activity against different insect species. Its toxicity relies on an internal 10 kDa peptide (pepcanatox), released by hydrolysis of Canatoxin by cathepsins in the digestive system of susceptible insects. In the present work, based on the N-terminal sequence of pepcanatox, we have designed primers to amplify by PCR a 270-bp fragment corresponding to pepcanatox using JBURE-II cDNA (one of the urease isoforms cloned from C. ensiformis, with high identity to JBURE-I, the classical urease) as a template. This amplicon named jaburetox-2 was cloned into pET 101 vector to obtain heterologous expression in Escherichia coli of the recombinant protein in C-terminal fusion with V-5 epitope and 6-His tag. Jaburetox-2Ec was purified on Nickel-NTA resin and bioassayed in insect models. Dysdercus peruvianus larvae were fed on cotton seed meal diets containing 0.01% (w/w) Jaburetox-2Ec and, after 11 days, all individuals were dead. Jaburetox-2Ec was also tested against Spodoptera frugiperda larvae and caused 100% mortality. In contrast, high doses of Jaburetox-2Ec were innocuous when injected or ingested by mice and neonate rats. Modeling of Jaburetox-2Ec, in comparison with other peptide structures, revealed a prominent b-hairpin motif consistent with an insecticidal activity based on either neurotoxicity or cell permeation.Made available in DSpace on 2016-10-10T03:53:07Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
title |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
spellingShingle |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis Mulinari, Fernanda Fontana Canatoxin Insecticide Spodoptera frugiperda Dysdercus peruvianus Molecular modeling Heterologous expression |
title_short |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
title_full |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
title_fullStr |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
title_full_unstemmed |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
title_sort |
Jaburetox-2Ec: An insecticidal peptide derived from an: isoform of urease from the plant canavalia ensiformis |
author |
Mulinari, Fernanda Fontana |
author_facet |
Mulinari, Fernanda Fontana Stanisçuaski, Fernanda Bertholdo-Vargas, Lúcia R. Postal, Melissa Oliveira-Neto, Osmundo Brilhante de Rigden, Daniel Jonh Grossi-de-Sá, Maria de Fátima Carlini, Celia Regina |
author_role |
author |
author2 |
Stanisçuaski, Fernanda Bertholdo-Vargas, Lúcia R. Postal, Melissa Oliveira-Neto, Osmundo Brilhante de Rigden, Daniel Jonh Grossi-de-Sá, Maria de Fátima Carlini, Celia Regina |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Mulinari, Fernanda Fontana Stanisçuaski, Fernanda Bertholdo-Vargas, Lúcia R. Postal, Melissa Oliveira-Neto, Osmundo Brilhante de Rigden, Daniel Jonh Grossi-de-Sá, Maria de Fátima Carlini, Celia Regina |
dc.subject.por.fl_str_mv |
Canatoxin Insecticide Spodoptera frugiperda Dysdercus peruvianus Molecular modeling Heterologous expression |
topic |
Canatoxin Insecticide Spodoptera frugiperda Dysdercus peruvianus Molecular modeling Heterologous expression |
dc.description.abstract.por.fl_txt_mv |
Canatoxin, a urease isoform from Canavalia ensiformis seeds, shows insecticidal activity against different insect species. Its toxicity relies on an internal 10 kDa peptide (pepcanatox), released by hydrolysis of Canatoxin by cathepsins in the digestive system of susceptible insects. In the present work, based on the N-terminal sequence of pepcanatox, we have designed primers to amplify by PCR a 270-bp fragment corresponding to pepcanatox using JBURE-II cDNA (one of the urease isoforms cloned from C. ensiformis, with high identity to JBURE-I, the classical urease) as a template. This amplicon named jaburetox-2 was cloned into pET 101 vector to obtain heterologous expression in Escherichia coli of the recombinant protein in C-terminal fusion with V-5 epitope and 6-His tag. Jaburetox-2Ec was purified on Nickel-NTA resin and bioassayed in insect models. Dysdercus peruvianus larvae were fed on cotton seed meal diets containing 0.01% (w/w) Jaburetox-2Ec and, after 11 days, all individuals were dead. Jaburetox-2Ec was also tested against Spodoptera frugiperda larvae and caused 100% mortality. In contrast, high doses of Jaburetox-2Ec were innocuous when injected or ingested by mice and neonate rats. Modeling of Jaburetox-2Ec, in comparison with other peptide structures, revealed a prominent b-hairpin motif consistent with an insecticidal activity based on either neurotoxicity or cell permeation. |
dc.description.version.pt_BR.fl_txt_mv |
Sim |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Canatoxin, a urease isoform from Canavalia ensiformis seeds, shows insecticidal activity against different insect species. Its toxicity relies on an internal 10 kDa peptide (pepcanatox), released by hydrolysis of Canatoxin by cathepsins in the digestive system of susceptible insects. In the present work, based on the N-terminal sequence of pepcanatox, we have designed primers to amplify by PCR a 270-bp fragment corresponding to pepcanatox using JBURE-II cDNA (one of the urease isoforms cloned from C. ensiformis, with high identity to JBURE-I, the classical urease) as a template. This amplicon named jaburetox-2 was cloned into pET 101 vector to obtain heterologous expression in Escherichia coli of the recombinant protein in C-terminal fusion with V-5 epitope and 6-His tag. Jaburetox-2Ec was purified on Nickel-NTA resin and bioassayed in insect models. Dysdercus peruvianus larvae were fed on cotton seed meal diets containing 0.01% (w/w) Jaburetox-2Ec and, after 11 days, all individuals were dead. Jaburetox-2Ec was also tested against Spodoptera frugiperda larvae and caused 100% mortality. In contrast, high doses of Jaburetox-2Ec were innocuous when injected or ingested by mice and neonate rats. Modeling of Jaburetox-2Ec, in comparison with other peptide structures, revealed a prominent b-hairpin motif consistent with an insecticidal activity based on either neurotoxicity or cell permeation. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-08 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:53:07Z |
dc.date.available.fl_str_mv |
2016-10-10T03:53:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
MULINARI, Fernanda Fontana, et al. Jaburetox-2Ec: an insecticidal peptide derived from an: isoform of urease from the plant Canavalia ensiformis. Peptides, v. 28, p. 2042- 2050, 2007. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/672 https://repositorio.ucb.br:9443/jspui/handle/123456789/7923 |
identifier_str_mv |
MULINARI, Fernanda Fontana, et al. Jaburetox-2Ec: an insecticidal peptide derived from an: isoform of urease from the plant Canavalia ensiformis. Peptides, v. 28, p. 2042- 2050, 2007. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/672 https://repositorio.ucb.br:9443/jspui/handle/123456789/7923 |
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eng |
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UCB |
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Repositório Institucional da UCB |
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Repositório Institucional da UCB |
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