Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance

Detalhes bibliográficos
Autor(a) principal: Costa, Christiane da Silva
Data de Publicação: 2005
Outros Autores: Albuquerque, Flávia Caixeta, Andrade, Rosângela Vieira, Oliveira, Gina Camilo de, Almeida, Mauro Fernandes de, Brigido, Marcelo de Macedo, Maranhão, Andrea Queiroz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/588
https://repositorio.ucb.br:9443/jspui/handle/123456789/7715
Resumo: In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the intracellular environment to the outside vicinity, using special transmembrane proteins, referred to as transporters. These proteins are also important for other reasons, since most of them are involved in nutrient uptake and cellular excretion. In cancer cells and in pathogens, and particularly in fungi, some of these proteins have been pointed out as responsible for an important phenotype known as multidrug resistance (MDR). In the present study, we tried to identify in the Paracoccidioides brasiliensis transcriptome, transporter-ortholog genes from the two major classes: ATP binding cassette and major facilitator superfamily transporter. We found 22 groups with good similarity with other fungal ATP binding cassette transporters, and four Paracoccidioides brasilienses assembled expressed sequence tags that probably code for major facilitator superfamily proteins. We also focused on fungicide resistance orthologs already characterized in other pathogenic fungi. We were able to find homologs to C. albicans CDR1, CDR2, and MDR1, Saccharomyces cerevisiae PDR5 and Aspergillus AtrF genes, all of them related to azole resistance. As current treatment for paracoccidioidomycosis mainly uses azole derivatives, the presence of these genes can be postulated to play a similar role in P. brasiliensis, warning us for the possibility of resistant isolate emergence.
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spelling Costa, Christiane da SilvaAlbuquerque, Flávia CaixetaAndrade, Rosângela VieiraOliveira, Gina Camilo deAlmeida, Mauro Fernandes deBrigido, Marcelo de MacedoMaranhão, Andrea Queiroz2016-10-10T03:52:28Z2016-10-10T03:52:28Z2005COSTA, Christiane da Silva et al. Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance. Genetics and Molecular Research, v. 4, p. 390-408, 2005.445280http://twingo.ucb.br:8080/jspui/handle/10869/588https://repositorio.ucb.br:9443/jspui/handle/123456789/7715In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the intracellular environment to the outside vicinity, using special transmembrane proteins, referred to as transporters. These proteins are also important for other reasons, since most of them are involved in nutrient uptake and cellular excretion. In cancer cells and in pathogens, and particularly in fungi, some of these proteins have been pointed out as responsible for an important phenotype known as multidrug resistance (MDR). In the present study, we tried to identify in the Paracoccidioides brasiliensis transcriptome, transporter-ortholog genes from the two major classes: ATP binding cassette and major facilitator superfamily transporter. We found 22 groups with good similarity with other fungal ATP binding cassette transporters, and four Paracoccidioides brasilienses assembled expressed sequence tags that probably code for major facilitator superfamily proteins. We also focused on fungicide resistance orthologs already characterized in other pathogenic fungi. We were able to find homologs to C. albicans CDR1, CDR2, and MDR1, Saccharomyces cerevisiae PDR5 and Aspergillus AtrF genes, all of them related to azole resistance. As current treatment for paracoccidioidomycosis mainly uses azole derivatives, the presence of these genes can be postulated to play a similar role in P. brasiliensis, warning us for the possibility of resistant isolate emergence.Made available in DSpace on 2016-10-10T03:52:28Z (GMT). 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dc.title.pt_BR.fl_str_mv Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
title Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
spellingShingle Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
Costa, Christiane da Silva
Drug resistance
Paracoccidioides brasiliensis
ATP binding cassette transporters
Major facilitator superfamily
title_short Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
title_full Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
title_fullStr Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
title_full_unstemmed Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
title_sort Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance
author Costa, Christiane da Silva
author_facet Costa, Christiane da Silva
Albuquerque, Flávia Caixeta
Andrade, Rosângela Vieira
Oliveira, Gina Camilo de
Almeida, Mauro Fernandes de
Brigido, Marcelo de Macedo
Maranhão, Andrea Queiroz
author_role author
author2 Albuquerque, Flávia Caixeta
Andrade, Rosângela Vieira
Oliveira, Gina Camilo de
Almeida, Mauro Fernandes de
Brigido, Marcelo de Macedo
Maranhão, Andrea Queiroz
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Christiane da Silva
Albuquerque, Flávia Caixeta
Andrade, Rosângela Vieira
Oliveira, Gina Camilo de
Almeida, Mauro Fernandes de
Brigido, Marcelo de Macedo
Maranhão, Andrea Queiroz
dc.subject.por.fl_str_mv Drug resistance
Paracoccidioides brasiliensis
ATP binding cassette transporters
Major facilitator superfamily
topic Drug resistance
Paracoccidioides brasiliensis
ATP binding cassette transporters
Major facilitator superfamily
dc.description.abstract.por.fl_txt_mv In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the intracellular environment to the outside vicinity, using special transmembrane proteins, referred to as transporters. These proteins are also important for other reasons, since most of them are involved in nutrient uptake and cellular excretion. In cancer cells and in pathogens, and particularly in fungi, some of these proteins have been pointed out as responsible for an important phenotype known as multidrug resistance (MDR). In the present study, we tried to identify in the Paracoccidioides brasiliensis transcriptome, transporter-ortholog genes from the two major classes: ATP binding cassette and major facilitator superfamily transporter. We found 22 groups with good similarity with other fungal ATP binding cassette transporters, and four Paracoccidioides brasilienses assembled expressed sequence tags that probably code for major facilitator superfamily proteins. We also focused on fungicide resistance orthologs already characterized in other pathogenic fungi. We were able to find homologs to C. albicans CDR1, CDR2, and MDR1, Saccharomyces cerevisiae PDR5 and Aspergillus AtrF genes, all of them related to azole resistance. As current treatment for paracoccidioidomycosis mainly uses azole derivatives, the presence of these genes can be postulated to play a similar role in P. brasiliensis, warning us for the possibility of resistant isolate emergence.
dc.description.version.pt_BR.fl_txt_mv Sim
dc.description.status.pt_BR.fl_txt_mv Publicado
description In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the intracellular environment to the outside vicinity, using special transmembrane proteins, referred to as transporters. These proteins are also important for other reasons, since most of them are involved in nutrient uptake and cellular excretion. In cancer cells and in pathogens, and particularly in fungi, some of these proteins have been pointed out as responsible for an important phenotype known as multidrug resistance (MDR). In the present study, we tried to identify in the Paracoccidioides brasiliensis transcriptome, transporter-ortholog genes from the two major classes: ATP binding cassette and major facilitator superfamily transporter. We found 22 groups with good similarity with other fungal ATP binding cassette transporters, and four Paracoccidioides brasilienses assembled expressed sequence tags that probably code for major facilitator superfamily proteins. We also focused on fungicide resistance orthologs already characterized in other pathogenic fungi. We were able to find homologs to C. albicans CDR1, CDR2, and MDR1, Saccharomyces cerevisiae PDR5 and Aspergillus AtrF genes, all of them related to azole resistance. As current treatment for paracoccidioidomycosis mainly uses azole derivatives, the presence of these genes can be postulated to play a similar role in P. brasiliensis, warning us for the possibility of resistant isolate emergence.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:28Z
dc.date.available.fl_str_mv 2016-10-10T03:52:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv COSTA, Christiane da Silva et al. Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance. Genetics and Molecular Research, v. 4, p. 390-408, 2005.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/588
https://repositorio.ucb.br:9443/jspui/handle/123456789/7715
dc.identifier.issn.none.fl_str_mv 445280
identifier_str_mv COSTA, Christiane da Silva et al. Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance. Genetics and Molecular Research, v. 4, p. 390-408, 2005.
445280
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