Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family
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Publication Date: | 2008 |
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Format: | Article |
Language: | por |
Source: | Repositório Institucional da UCB |
Download full: | http://twingo.ucb.br:8080/jspui/handle/10869/616 https://repositorio.ucb.br:9443/jspui/handle/123456789/7819 |
Summary: | Plant defensins are small cysteine-rich proteins commonly synthesized in plants, encoded by large multigene families. Most plant defensins that have been characterized to date show potent antifungal and/or bactericidal activities. This report describes VuD1, an unusual defensin that is able to inhibit insect-pest a-amylases. VuD1 was cloned from cowpea (Vigna unguiculata) seeds and expressed in a heterologous system. Inhibitory enzyme assays showed that VuD1 efficiently inhibits a-amylases from the weevils Acanthoscelides obtectus and Zabrotes subfasciatus, caused low inhibition toward mammalian enzymes and was unable to inhibit the a-amylases from Callosobruchus maculatus and Aspergillus fumigatus. To shed some light over the mechanism of action of VuD1, molecular modeling analyses were performed, revealing that the N-terminus of the molecule is responsible for binding with the active site of weevil enzymes. Moreover, models of VuD1 and mammalian enzymes were also generated to elucidate the specificity mechanisms. The data presented herein suggests that this defensin has potential application in the development of transgenic plants for insect pest control. |
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Pelegrini, Patrícia B.Fung, T. LayMurad, André M.Anderson, Marilyn A.Franco, Octavio L.2016-10-10T03:52:47Z2016-10-10T03:52:47Z2008PELEGRINI, Patrícia B.et al. Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family. Proteins. v. 73, p. 719–729, 2008.http://twingo.ucb.br:8080/jspui/handle/10869/616https://repositorio.ucb.br:9443/jspui/handle/123456789/7819Plant defensins are small cysteine-rich proteins commonly synthesized in plants, encoded by large multigene families. Most plant defensins that have been characterized to date show potent antifungal and/or bactericidal activities. This report describes VuD1, an unusual defensin that is able to inhibit insect-pest a-amylases. VuD1 was cloned from cowpea (Vigna unguiculata) seeds and expressed in a heterologous system. Inhibitory enzyme assays showed that VuD1 efficiently inhibits a-amylases from the weevils Acanthoscelides obtectus and Zabrotes subfasciatus, caused low inhibition toward mammalian enzymes and was unable to inhibit the a-amylases from Callosobruchus maculatus and Aspergillus fumigatus. To shed some light over the mechanism of action of VuD1, molecular modeling analyses were performed, revealing that the N-terminus of the molecule is responsible for binding with the active site of weevil enzymes. Moreover, models of VuD1 and mammalian enzymes were also generated to elucidate the specificity mechanisms. The data presented herein suggests that this defensin has potential application in the development of transgenic plants for insect pest control.Made available in DSpace on 2016-10-10T03:52:47Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
title |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
spellingShingle |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family Pelegrini, Patrícia B. Cowpea Defensin Cloning Expression A-Amylase |
title_short |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
title_full |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
title_fullStr |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
title_full_unstemmed |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
title_sort |
Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family |
author |
Pelegrini, Patrícia B. |
author_facet |
Pelegrini, Patrícia B. Fung, T. Lay Murad, André M. Anderson, Marilyn A. Franco, Octavio L. |
author_role |
author |
author2 |
Fung, T. Lay Murad, André M. Anderson, Marilyn A. Franco, Octavio L. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pelegrini, Patrícia B. Fung, T. Lay Murad, André M. Anderson, Marilyn A. Franco, Octavio L. |
dc.subject.por.fl_str_mv |
Cowpea Defensin Cloning Expression A-Amylase |
topic |
Cowpea Defensin Cloning Expression A-Amylase |
dc.description.abstract.por.fl_txt_mv |
Plant defensins are small cysteine-rich proteins commonly synthesized in plants, encoded by large multigene families. Most plant defensins that have been characterized to date show potent antifungal and/or bactericidal activities. This report describes VuD1, an unusual defensin that is able to inhibit insect-pest a-amylases. VuD1 was cloned from cowpea (Vigna unguiculata) seeds and expressed in a heterologous system. Inhibitory enzyme assays showed that VuD1 efficiently inhibits a-amylases from the weevils Acanthoscelides obtectus and Zabrotes subfasciatus, caused low inhibition toward mammalian enzymes and was unable to inhibit the a-amylases from Callosobruchus maculatus and Aspergillus fumigatus. To shed some light over the mechanism of action of VuD1, molecular modeling analyses were performed, revealing that the N-terminus of the molecule is responsible for binding with the active site of weevil enzymes. Moreover, models of VuD1 and mammalian enzymes were also generated to elucidate the specificity mechanisms. The data presented herein suggests that this defensin has potential application in the development of transgenic plants for insect pest control. |
dc.description.version.pt_BR.fl_txt_mv |
Sim |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Plant defensins are small cysteine-rich proteins commonly synthesized in plants, encoded by large multigene families. Most plant defensins that have been characterized to date show potent antifungal and/or bactericidal activities. This report describes VuD1, an unusual defensin that is able to inhibit insect-pest a-amylases. VuD1 was cloned from cowpea (Vigna unguiculata) seeds and expressed in a heterologous system. Inhibitory enzyme assays showed that VuD1 efficiently inhibits a-amylases from the weevils Acanthoscelides obtectus and Zabrotes subfasciatus, caused low inhibition toward mammalian enzymes and was unable to inhibit the a-amylases from Callosobruchus maculatus and Aspergillus fumigatus. To shed some light over the mechanism of action of VuD1, molecular modeling analyses were performed, revealing that the N-terminus of the molecule is responsible for binding with the active site of weevil enzymes. Moreover, models of VuD1 and mammalian enzymes were also generated to elucidate the specificity mechanisms. The data presented herein suggests that this defensin has potential application in the development of transgenic plants for insect pest control. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:52:47Z |
dc.date.available.fl_str_mv |
2016-10-10T03:52:47Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
PELEGRINI, Patrícia B.et al. Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family. Proteins. v. 73, p. 719–729, 2008. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/616 https://repositorio.ucb.br:9443/jspui/handle/123456789/7819 |
identifier_str_mv |
PELEGRINI, Patrícia B.et al. Novel insights on the mechanism of action of a-amylase inhibitors from the plant defensin family. Proteins. v. 73, p. 719–729, 2008. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/616 https://repositorio.ucb.br:9443/jspui/handle/123456789/7819 |
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