ANALYSIS OF CREATINE KINASE AND ALPHA-ACTIN CONCENTRATIONS IN SOCCER PRE-SEASON DOI: http://dx.doi.org/10.18511/0103-1716/rbcm.v23n4p5-14

• Autor(a) principal: Pimenta, Eduardo
• Data de Publicação: 2015
• Outros Autores: Coelho, Daniel Barbosa, Capettini, Luciano, Gomes, Thales, Pussieldi, Guilherme, Ribeiro, Jose, Gomes-Filho, Ary, Silami-Garcia, Emerson
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Brasileira de Ciência e Movimento (Online)
• Texto Completo: https://portalrevistas.ucb.br/index.php/rbcm/article/view/4979
• topic: Muscle damage; creatine kinase; training monitoring.

DOI: http://dx.doi.org/10.18511/0103-1716/rbcm.v23n4p5-14 Purpose: The purpose of the present study was to assess muscle overload through changes in creatine kinase (CK) activity and serum concentration of ?-actin in soccer players in response of two months of pre-season physical training. Basic procedures: Ten professional athletes (26 ± 6 years of age) undergoing arduous training were evaluated. Serum CK activity and ?-actin serum concentration were determined at the beginning and end of two months of pre-season training. Effort intensity was determined throughout the training sessions by monitoring heart rate (HR). Serum CK activity was determined using the colorimetric method. Plasma ?-actin concentration was evaluated using ELISA and confirmed using the Western Blot method. Main Findings: Serum CK activity and ?-actin concentration were higher in the post-training period in comparison to the pre-training period (CK: PRE 65.5 ± 4.2 U/L, POST 435.7 ± 70.0 U/L, p=0.0004; ?-actin by Western Blot: PRE 42.9 ± 21.6 ?g/mL, POST 68.1 ± 19.1 ?g/mL, p=0.0004; by ELISA: PRE 70.63 ± 10.4 ?g/mL, POST 101.80 ± 17.3 ?g/mL, p=0.0125). Conclusions: The increase in CK activity and ?-actin concentration over two months of pre-season training of a soccer team indicates a significant muscle overload imposed by this training period. Thus, ?-actin and CK are factors for monitoring early muscle overload in the two-month physical preparation of a professional soccer team.