Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.

Detalhes bibliográficos
Autor(a) principal: Lemus, Sara do Nascimento
Data de Publicação: 2022
Outros Autores: Mourão, Luís Paulo Bueno, Ferrari, Elís Domingos, Bresciani, Katia Denise Saraiva, Silveira Neto, Luiz da, Pimenta, Raphael Sanzio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45080
Resumo: In this study, we investigated the occurrence and characterized molecularly Cryptosporidium oocysts in feces of dogs seroreactive for Leishmania spp. We hypothesized that the clinical staging of canine visceral leishmaniasis (CVL) influences the occurrence and intensity of Cryptosporidium spp. oocyst shedding in feces from the canine host due to the immunosuppression induced by Leishmania infantum. Fecal samples from 101 dogs with anti-Leishmania spp. antibodies detected by Dual-Path Platform (DPP®) immunochromatographic test (Biomanguinhos) and/or enzyme-linked immunosorbent assay (Biomanguinhos) were concentrated by the centrifuge-sedimentation method in water-ethyl acetate. The sediments were submitted to the technique of negative staining with malachite green and observed under an optical microscope (400× magnification). The occurrence was 0.99% (95 confidence interval 0.00%-2.93%). A single Cryptosporidium oocyst was found in one of the samples examined by microscopy, and the result was confirmed by nested-PCR. The amplicon sequence showed 100% genetic identity with Cryptosporidium canis. The dog presented the following clinical signs suggestive of CVL: cachexia, generalized alopecia, pale colored gingival mucosa, splenomegaly, and onychogryphosis. We conclude that there is no evidence that the oligosymptomatic and symptomatic clinical staging of dogs seroreactive for Leishmania spp. influenced the occurrence and intensity of C. canis oocyst shedding in the canine host.
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spelling Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.Baixa ocorrência de Cryptosporidium canis em fezes de cães soropositivos para Leishmania spp.CryptosporidiosisEpidemiologyLeishmaniasisOne healthZoonosis.CriptosporidioseEpidemiologiaLeishmanioseSaúde únicaZoonose.In this study, we investigated the occurrence and characterized molecularly Cryptosporidium oocysts in feces of dogs seroreactive for Leishmania spp. We hypothesized that the clinical staging of canine visceral leishmaniasis (CVL) influences the occurrence and intensity of Cryptosporidium spp. oocyst shedding in feces from the canine host due to the immunosuppression induced by Leishmania infantum. Fecal samples from 101 dogs with anti-Leishmania spp. antibodies detected by Dual-Path Platform (DPP®) immunochromatographic test (Biomanguinhos) and/or enzyme-linked immunosorbent assay (Biomanguinhos) were concentrated by the centrifuge-sedimentation method in water-ethyl acetate. The sediments were submitted to the technique of negative staining with malachite green and observed under an optical microscope (400× magnification). The occurrence was 0.99% (95 confidence interval 0.00%-2.93%). A single Cryptosporidium oocyst was found in one of the samples examined by microscopy, and the result was confirmed by nested-PCR. The amplicon sequence showed 100% genetic identity with Cryptosporidium canis. The dog presented the following clinical signs suggestive of CVL: cachexia, generalized alopecia, pale colored gingival mucosa, splenomegaly, and onychogryphosis. We conclude that there is no evidence that the oligosymptomatic and symptomatic clinical staging of dogs seroreactive for Leishmania spp. influenced the occurrence and intensity of C. canis oocyst shedding in the canine host.Neste estudo, nós investigamos a ocorrência e caracterizamos molecularmente oocistos de Cryptosporidium em fezes de cães sororreativos para Leishmania spp. Nossa hipótese era que o estadiamento clínico da leishmaniose visceral canina (LVC) influenciasse na ocorrência e intensidade de eliminação de oocistos de Cryptosporidium spp. em fezes do hospedeiro canino devido à imunossupressão induzida por Leishmania infantum. Amostras fecais de 101 cães com anticorpos anti-Leishmania spp. detectados pelo teste imunocromatográfico Dual-Path Platform (DPP®) (Biomanguinhos) e/ou ensaio imunoenzimático (Biomanguinhos) foram concentrados pelo método de centrífugo-sedimentação em água-acetato de etila. Os sedimentos foram submetidos à técnica de coloração negativa por verde malaquita e observados ao microscópio óptico (ampliação de 400x). A ocorrência foi de 0,99% (intervalo de confiança de 95%: 0,0 - 2,93%). Um único oocisto de Cryptosporidium foi encontrado em uma das amostras examinadas por microscopia, e o resultado foi confirmado por nested-PCR. A sequência do amplicon apresentou 100% de identidade genética com Cryptosporidium canis. O cão apresentava os seguintes sinais clínicos sugestivos de LVC: caquexia, alopecia generalizada, mucosa gengival pálida, esplenomegalia e onicogrifose. Concluímos que não há evidências de que o estadiamento clínico oligossintomático e sintomático de cães sororreativos para Leishmania spp. influencie na ocorrência e intensidade de excreção de oocistos de C. canis em fezes do hospedeiro canino.UEL2022-05-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4508010.5433/1679-0359.2022v43n4p1823Semina: Ciências Agrárias; Vol. 43 No. 4 (2022); 1823-1834Semina: Ciências Agrárias; v. 43 n. 4 (2022); 1823-18341679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45080/32038Copyright (c) 2022 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLemus, Sara do NascimentoMourão, Luís Paulo BuenoFerrari, Elís DomingosBresciani, Katia Denise SaraivaSilveira Neto, Luiz daPimenta, Raphael Sanzio2022-09-16T23:21:39Zoai:ojs.pkp.sfu.ca:article/45080Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-16T23:21:39Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
Baixa ocorrência de Cryptosporidium canis em fezes de cães soropositivos para Leishmania spp.
title Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
spellingShingle Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
Lemus, Sara do Nascimento
Cryptosporidiosis
Epidemiology
Leishmaniasis
One health
Zoonosis.
Criptosporidiose
Epidemiologia
Leishmaniose
Saúde única
Zoonose.
title_short Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
title_full Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
title_fullStr Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
title_full_unstemmed Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
title_sort Low occurrence of Cryptosporidium canis in feces of dogs seroreactive for Leishmania spp.
author Lemus, Sara do Nascimento
author_facet Lemus, Sara do Nascimento
Mourão, Luís Paulo Bueno
Ferrari, Elís Domingos
Bresciani, Katia Denise Saraiva
Silveira Neto, Luiz da
Pimenta, Raphael Sanzio
author_role author
author2 Mourão, Luís Paulo Bueno
Ferrari, Elís Domingos
Bresciani, Katia Denise Saraiva
Silveira Neto, Luiz da
Pimenta, Raphael Sanzio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lemus, Sara do Nascimento
Mourão, Luís Paulo Bueno
Ferrari, Elís Domingos
Bresciani, Katia Denise Saraiva
Silveira Neto, Luiz da
Pimenta, Raphael Sanzio
dc.subject.por.fl_str_mv Cryptosporidiosis
Epidemiology
Leishmaniasis
One health
Zoonosis.
Criptosporidiose
Epidemiologia
Leishmaniose
Saúde única
Zoonose.
topic Cryptosporidiosis
Epidemiology
Leishmaniasis
One health
Zoonosis.
Criptosporidiose
Epidemiologia
Leishmaniose
Saúde única
Zoonose.
description In this study, we investigated the occurrence and characterized molecularly Cryptosporidium oocysts in feces of dogs seroreactive for Leishmania spp. We hypothesized that the clinical staging of canine visceral leishmaniasis (CVL) influences the occurrence and intensity of Cryptosporidium spp. oocyst shedding in feces from the canine host due to the immunosuppression induced by Leishmania infantum. Fecal samples from 101 dogs with anti-Leishmania spp. antibodies detected by Dual-Path Platform (DPP®) immunochromatographic test (Biomanguinhos) and/or enzyme-linked immunosorbent assay (Biomanguinhos) were concentrated by the centrifuge-sedimentation method in water-ethyl acetate. The sediments were submitted to the technique of negative staining with malachite green and observed under an optical microscope (400× magnification). The occurrence was 0.99% (95 confidence interval 0.00%-2.93%). A single Cryptosporidium oocyst was found in one of the samples examined by microscopy, and the result was confirmed by nested-PCR. The amplicon sequence showed 100% genetic identity with Cryptosporidium canis. The dog presented the following clinical signs suggestive of CVL: cachexia, generalized alopecia, pale colored gingival mucosa, splenomegaly, and onychogryphosis. We conclude that there is no evidence that the oligosymptomatic and symptomatic clinical staging of dogs seroreactive for Leishmania spp. influenced the occurrence and intensity of C. canis oocyst shedding in the canine host.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45080
10.5433/1679-0359.2022v43n4p1823
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45080
identifier_str_mv 10.5433/1679-0359.2022v43n4p1823
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45080/32038
dc.rights.driver.fl_str_mv Copyright (c) 2022 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 43 No. 4 (2022); 1823-1834
Semina: Ciências Agrárias; v. 43 n. 4 (2022); 1823-1834
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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