Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis

Detalhes bibliográficos
Autor(a) principal: Veloso, Jéssica Fontes
Data de Publicação: 2018
Outros Autores: Sauer, Leonardo, Oriá, Arianne Pontes, Gomes Junior, Deusdete Conceição, Raposo, Ana Cláudia Santos, Andrade, Camila Fernanda Oliveira, Oliveira, Thais Nascimento de Andrade, Carlos, Renata Santiago Alberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28704
Resumo: Canine monocytic ehrlichiosis (CME) is an infectious disease caused by a gram-negative bacterium Ehrlichia canis that has a high global prevalence that leads to high rates of morbidity and mortality in dogs. Among the clinical changes, ophthalmic diseases can lead to permanent blindness and it can be an important clinical sign. The objective of this study was to perform nested polymerase chain reaction (PCR) to diagnose E. canis infection in dogs with bilateral uveitis from the Veterinary Hospital of the Santa Cruz State University. Blood samples were collected and DNA for the molecular diagnosis was extracted from 66 adult dogs of both genders and mixed breeds diagnosed with bilateral uveitis. Thirty-five (53%) dogs showed positive results and presented with iridocyclitis, posterior uveitis, panuveitis, or uveitis with secondary glaucoma. This study demonstrates that nested PCR is an important tool for the differential diagnosis of dogs with bilateral uveitis, as it provides evidence of the infectious agent in the animal.
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spelling Molecular diagnosis of Ehrlichia canis infection in dogs with uveitisDiagnóstico molecular da infecção por Ehrlichia canis em cães com uveíteEyeGlaucomaInfectious UveitisNested PCRRickettsia.OlhoGlaucomaUveíte infecciosaNested PCRRickettsia.Canine monocytic ehrlichiosis (CME) is an infectious disease caused by a gram-negative bacterium Ehrlichia canis that has a high global prevalence that leads to high rates of morbidity and mortality in dogs. Among the clinical changes, ophthalmic diseases can lead to permanent blindness and it can be an important clinical sign. The objective of this study was to perform nested polymerase chain reaction (PCR) to diagnose E. canis infection in dogs with bilateral uveitis from the Veterinary Hospital of the Santa Cruz State University. Blood samples were collected and DNA for the molecular diagnosis was extracted from 66 adult dogs of both genders and mixed breeds diagnosed with bilateral uveitis. Thirty-five (53%) dogs showed positive results and presented with iridocyclitis, posterior uveitis, panuveitis, or uveitis with secondary glaucoma. This study demonstrates that nested PCR is an important tool for the differential diagnosis of dogs with bilateral uveitis, as it provides evidence of the infectious agent in the animal.Erliquiose Monocítica Canina (EMC) é uma doença infecciosa causada por uma bactéria gram negativa da espécie Ehrlichia canis, que possui alta prevalência mundial e conduz a elevadas taxas de morbidade e mortalidade em cães. Dentre as alterações clínicas, as oftalmopatias podem levar a cegueira permanente e é ser um importante sinal clínico. Objetivou-se realizar nested-PCR para diagnosticar infecção por E. canis em cães portadores de uveíte bilateral provenientes da casuística do Hospital Veterinário da Universidade Estadual de Santa Cruz. Sessenta e seis cães adultos, de ambos sexos e raças variadas, diagnosticados com uveíte bilateral foram submetidos a coleta de sangue e o DNA para realização do diagnóstico molecular foi extraído destas amostras. Foram positivos no teste 35 (53%) cães, que apresentaram iridociclite, uveíte posterior, panuveíte ou uveíte com glaucoma secundário. Esse estudo demonstra a nested-PCR como ferramenta importante no diagnóstico diferencial de cães com uveíte bilateral, por proporcionar a comprovação do agente infeccioso no organismo do animal.UEL2018-05-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo Científicoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2870410.5433/1679-0359.2018v39n3p1049Semina: Ciências Agrárias; Vol. 39 No. 3 (2018); 1049-1056Semina: Ciências Agrárias; v. 39 n. 3 (2018); 1049-10561679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28704/23501Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessVeloso, Jéssica FontesSauer, LeonardoOriá, Arianne PontesGomes Junior, Deusdete ConceiçãoRaposo, Ana Cláudia SantosAndrade, Camila Fernanda OliveiraOliveira, Thais Nascimento de AndradeCarlos, Renata Santiago Alberto2022-10-20T20:42:35Zoai:ojs.pkp.sfu.ca:article/28704Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-20T20:42:35Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
Diagnóstico molecular da infecção por Ehrlichia canis em cães com uveíte
title Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
spellingShingle Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
Veloso, Jéssica Fontes
Eye
Glaucoma
Infectious Uveitis
Nested PCR
Rickettsia.
Olho
Glaucoma
Uveíte infecciosa
Nested PCR
Rickettsia.
title_short Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
title_full Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
title_fullStr Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
title_full_unstemmed Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
title_sort Molecular diagnosis of Ehrlichia canis infection in dogs with uveitis
author Veloso, Jéssica Fontes
author_facet Veloso, Jéssica Fontes
Sauer, Leonardo
Oriá, Arianne Pontes
Gomes Junior, Deusdete Conceição
Raposo, Ana Cláudia Santos
Andrade, Camila Fernanda Oliveira
Oliveira, Thais Nascimento de Andrade
Carlos, Renata Santiago Alberto
author_role author
author2 Sauer, Leonardo
Oriá, Arianne Pontes
Gomes Junior, Deusdete Conceição
Raposo, Ana Cláudia Santos
Andrade, Camila Fernanda Oliveira
Oliveira, Thais Nascimento de Andrade
Carlos, Renata Santiago Alberto
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Veloso, Jéssica Fontes
Sauer, Leonardo
Oriá, Arianne Pontes
Gomes Junior, Deusdete Conceição
Raposo, Ana Cláudia Santos
Andrade, Camila Fernanda Oliveira
Oliveira, Thais Nascimento de Andrade
Carlos, Renata Santiago Alberto
dc.subject.por.fl_str_mv Eye
Glaucoma
Infectious Uveitis
Nested PCR
Rickettsia.
Olho
Glaucoma
Uveíte infecciosa
Nested PCR
Rickettsia.
topic Eye
Glaucoma
Infectious Uveitis
Nested PCR
Rickettsia.
Olho
Glaucoma
Uveíte infecciosa
Nested PCR
Rickettsia.
description Canine monocytic ehrlichiosis (CME) is an infectious disease caused by a gram-negative bacterium Ehrlichia canis that has a high global prevalence that leads to high rates of morbidity and mortality in dogs. Among the clinical changes, ophthalmic diseases can lead to permanent blindness and it can be an important clinical sign. The objective of this study was to perform nested polymerase chain reaction (PCR) to diagnose E. canis infection in dogs with bilateral uveitis from the Veterinary Hospital of the Santa Cruz State University. Blood samples were collected and DNA for the molecular diagnosis was extracted from 66 adult dogs of both genders and mixed breeds diagnosed with bilateral uveitis. Thirty-five (53%) dogs showed positive results and presented with iridocyclitis, posterior uveitis, panuveitis, or uveitis with secondary glaucoma. This study demonstrates that nested PCR is an important tool for the differential diagnosis of dogs with bilateral uveitis, as it provides evidence of the infectious agent in the animal.
publishDate 2018
dc.date.none.fl_str_mv 2018-05-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo Científico
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28704
10.5433/1679-0359.2018v39n3p1049
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28704
identifier_str_mv 10.5433/1679-0359.2018v39n3p1049
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28704/23501
dc.rights.driver.fl_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 39 No. 3 (2018); 1049-1056
Semina: Ciências Agrárias; v. 39 n. 3 (2018); 1049-1056
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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