Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Bertolini, Amanda Bezerra
Data de Publicação: 2019
Outros Autores: Dutra, Marcelo Augusto Orsi, Guiraldi, Lívia Maísa, Santos, Wesley José dos, Medeiros, Maria Izabel Merino de, Lucheis, Simone Baldini
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875
Resumo: Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment.
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spelling Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, BrazilIsolamento e identificação de Yersinia enterocolitica em amostras de leite cru bovino a partir de tanques de expansão, São Paulo, BrasilMicrobiological analysesMolecular diagnosisBovine milkPublic healthYersiniosis.Análise microbiológicaDiagnóstico molecularLeite bovinoSaúde públicaYersiniose.Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment.O leite e seus derivados possuem substratos que favorecem o desenvolvimento de micro-organismos patogênicos e deteriorantes, devido a isso, cuidados rigorosos são exigindo durante a ordenha, processamento e seu armazenamento. Dentre os vários grupos de bactérias que podem contaminar o leite refrigerado cru está Yersinia enterocolitica. Esta bactéria pode causar a uma série de sintomas clínicos intestinais e extraintestinais, variando de gastroenterite leve a linfadenite mesentérica, semelhante à apendicite. Para avaliar a prevalência de Y. enterocolitica patogênica no leite cru de tanques de expansão localizados no estado de São Paulo, foram avaliadas 102 amostras de leite bovino, por análises microbiológicas, seguido de provas bioquímicas; a Reação em Cadeia de Polimerase (PCR) e sequenciamento genético. Não houve o isolamento de Y. enterocolitica pelas provas microbiológicas clássicas. No entanto, a análise de PCR, realizada diretamente do leite, revelou seis (6) amostras positivas para Y. enterocolitica (5,9%), confirmadas por sequenciamento genético. Somente o gene inv, foi detectado, que pode estar presente em cepas virulentas e avirulentas. Houve dificuldade de isolamento microbiológico devido à dificuldade de competitividade da Y. enterocolitica em uma microbiota muito rica do leite cru. Ainda que não tenham sido identificados os genes de virulência que sabidamente estão presentes em cepas de Y. enterocolitica potencialmente patogênicas, a presença desse patógeno no leite dos tanques de expansão, identificadas através da PCR e confirmadas por sequenciamento genético, sugere que Yersinia enterocolitica pode ser um risco potencial para a saúde pública, principalmente se o leite e seus derivados forem consumidos sem tratamento térmico.UEL2019-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionby microbiological analysis, followed by biochemical tests including PCR and genetic sequencingapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3387510.5433/1679-0359.2019v40n2p723Semina: Ciências Agrárias; Vol. 40 No. 2 (2019); 723-730Semina: Ciências Agrárias; v. 40 n. 2 (2019); 723-7301679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875/25259Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBertolini, Amanda BezerraDutra, Marcelo Augusto OrsiGuiraldi, Lívia MaísaSantos, Wesley José dosMedeiros, Maria Izabel Merino deLucheis, Simone Baldini2022-10-19T14:20:08Zoai:ojs.pkp.sfu.ca:article/33875Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T14:20:08Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
Isolamento e identificação de Yersinia enterocolitica em amostras de leite cru bovino a partir de tanques de expansão, São Paulo, Brasil
title Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
spellingShingle Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
Bertolini, Amanda Bezerra
Microbiological analyses
Molecular diagnosis
Bovine milk
Public health
Yersiniosis.
Análise microbiológica
Diagnóstico molecular
Leite bovino
Saúde pública
Yersiniose.
title_short Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
title_full Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
title_fullStr Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
title_full_unstemmed Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
title_sort Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
author Bertolini, Amanda Bezerra
author_facet Bertolini, Amanda Bezerra
Dutra, Marcelo Augusto Orsi
Guiraldi, Lívia Maísa
Santos, Wesley José dos
Medeiros, Maria Izabel Merino de
Lucheis, Simone Baldini
author_role author
author2 Dutra, Marcelo Augusto Orsi
Guiraldi, Lívia Maísa
Santos, Wesley José dos
Medeiros, Maria Izabel Merino de
Lucheis, Simone Baldini
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bertolini, Amanda Bezerra
Dutra, Marcelo Augusto Orsi
Guiraldi, Lívia Maísa
Santos, Wesley José dos
Medeiros, Maria Izabel Merino de
Lucheis, Simone Baldini
dc.subject.por.fl_str_mv Microbiological analyses
Molecular diagnosis
Bovine milk
Public health
Yersiniosis.
Análise microbiológica
Diagnóstico molecular
Leite bovino
Saúde pública
Yersiniose.
topic Microbiological analyses
Molecular diagnosis
Bovine milk
Public health
Yersiniosis.
Análise microbiológica
Diagnóstico molecular
Leite bovino
Saúde pública
Yersiniose.
description Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
by microbiological analysis, followed by biochemical tests including PCR and genetic sequencing
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875
10.5433/1679-0359.2019v40n2p723
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875
identifier_str_mv 10.5433/1679-0359.2019v40n2p723
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875/25259
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 2 (2019); 723-730
Semina: Ciências Agrárias; v. 40 n. 2 (2019); 723-730
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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