Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875 |
Resumo: | Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment. |
id |
UEL-11_2360362567909bfd0b7ed29b1204c262 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/33875 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, BrazilIsolamento e identificação de Yersinia enterocolitica em amostras de leite cru bovino a partir de tanques de expansão, São Paulo, BrasilMicrobiological analysesMolecular diagnosisBovine milkPublic healthYersiniosis.Análise microbiológicaDiagnóstico molecularLeite bovinoSaúde públicaYersiniose.Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment.O leite e seus derivados possuem substratos que favorecem o desenvolvimento de micro-organismos patogênicos e deteriorantes, devido a isso, cuidados rigorosos são exigindo durante a ordenha, processamento e seu armazenamento. Dentre os vários grupos de bactérias que podem contaminar o leite refrigerado cru está Yersinia enterocolitica. Esta bactéria pode causar a uma série de sintomas clínicos intestinais e extraintestinais, variando de gastroenterite leve a linfadenite mesentérica, semelhante à apendicite. Para avaliar a prevalência de Y. enterocolitica patogênica no leite cru de tanques de expansão localizados no estado de São Paulo, foram avaliadas 102 amostras de leite bovino, por análises microbiológicas, seguido de provas bioquímicas; a Reação em Cadeia de Polimerase (PCR) e sequenciamento genético. Não houve o isolamento de Y. enterocolitica pelas provas microbiológicas clássicas. No entanto, a análise de PCR, realizada diretamente do leite, revelou seis (6) amostras positivas para Y. enterocolitica (5,9%), confirmadas por sequenciamento genético. Somente o gene inv, foi detectado, que pode estar presente em cepas virulentas e avirulentas. Houve dificuldade de isolamento microbiológico devido à dificuldade de competitividade da Y. enterocolitica em uma microbiota muito rica do leite cru. Ainda que não tenham sido identificados os genes de virulência que sabidamente estão presentes em cepas de Y. enterocolitica potencialmente patogênicas, a presença desse patógeno no leite dos tanques de expansão, identificadas através da PCR e confirmadas por sequenciamento genético, sugere que Yersinia enterocolitica pode ser um risco potencial para a saúde pública, principalmente se o leite e seus derivados forem consumidos sem tratamento térmico.UEL2019-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionby microbiological analysis, followed by biochemical tests including PCR and genetic sequencingapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3387510.5433/1679-0359.2019v40n2p723Semina: Ciências Agrárias; Vol. 40 No. 2 (2019); 723-730Semina: Ciências Agrárias; v. 40 n. 2 (2019); 723-7301679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875/25259Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBertolini, Amanda BezerraDutra, Marcelo Augusto OrsiGuiraldi, Lívia MaísaSantos, Wesley José dosMedeiros, Maria Izabel Merino deLucheis, Simone Baldini2022-10-19T14:20:08Zoai:ojs.pkp.sfu.ca:article/33875Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T14:20:08Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil Isolamento e identificação de Yersinia enterocolitica em amostras de leite cru bovino a partir de tanques de expansão, São Paulo, Brasil |
title |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil |
spellingShingle |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil Bertolini, Amanda Bezerra Microbiological analyses Molecular diagnosis Bovine milk Public health Yersiniosis. Análise microbiológica Diagnóstico molecular Leite bovino Saúde pública Yersiniose. |
title_short |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil |
title_full |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil |
title_fullStr |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil |
title_full_unstemmed |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil |
title_sort |
Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil |
author |
Bertolini, Amanda Bezerra |
author_facet |
Bertolini, Amanda Bezerra Dutra, Marcelo Augusto Orsi Guiraldi, Lívia Maísa Santos, Wesley José dos Medeiros, Maria Izabel Merino de Lucheis, Simone Baldini |
author_role |
author |
author2 |
Dutra, Marcelo Augusto Orsi Guiraldi, Lívia Maísa Santos, Wesley José dos Medeiros, Maria Izabel Merino de Lucheis, Simone Baldini |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bertolini, Amanda Bezerra Dutra, Marcelo Augusto Orsi Guiraldi, Lívia Maísa Santos, Wesley José dos Medeiros, Maria Izabel Merino de Lucheis, Simone Baldini |
dc.subject.por.fl_str_mv |
Microbiological analyses Molecular diagnosis Bovine milk Public health Yersiniosis. Análise microbiológica Diagnóstico molecular Leite bovino Saúde pública Yersiniose. |
topic |
Microbiological analyses Molecular diagnosis Bovine milk Public health Yersiniosis. Análise microbiológica Diagnóstico molecular Leite bovino Saúde pública Yersiniose. |
description |
Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion by microbiological analysis, followed by biochemical tests including PCR and genetic sequencing |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875 10.5433/1679-0359.2019v40n2p723 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875 |
identifier_str_mv |
10.5433/1679-0359.2019v40n2p723 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33875/25259 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 2 (2019); 723-730 Semina: Ciências Agrárias; v. 40 n. 2 (2019); 723-730 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306080314982400 |