Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus

Detalhes bibliográficos
Autor(a) principal: Santos, Alessandro José Ferreira dos
Data de Publicação: 2019
Outros Autores: Almeida, Katyane de Sousa, Baptista, Francisco, Silva, Marco Augusto Giannoccaro da, Alexandrino, Bruna, Ferreira, Jardel Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32801
Resumo: Bovine brucellosis is transmitted by Brucella abortus and causes considerable economic losses to agribusiness. To determine the seroconversion rate attributable to vaccination of heifers with B. abortus strain B-19, 330 blood serum samples from 110 heifers aged three to eight months from five properties in the municipality of Araguaína, Tocantins, Brazil, were analyzed. On day zero (D-0), blood samples were collected, and 55 heifers from the intervention group were vaccinated. The remaining blood samples were collected on days seven (D-7), 14 (D-14), and 21 (D-21). The serum samples were analyzed using the buffered acidified plate antigen (BAPA) test. The rate of seroconversion attributable to vaccination was calculated by subtracting from the seroconversion incidence rate in the intervention group the incidence rate of the control group. On D-0, 100% (110/110) of the heifers were negative in the BAPA test (95% CI [96.70 - 100%], p < 0.05). On D-7, the seroconversion rate was 94.55% (52/55) (95% CI [84.88 - 98.84%], p < 0.05) in the intervention group and 0% (0/55) (95% CI [0.00 - 6.49%], p < 0.05) in the control group. On D-14, the rate was 98.18% (54/55) (95% CI [90.28 - 99.95%], p < 0.05) in the intervention group and 0% in the control group. Therefore, seroconversion attributable to vaccination varied from 78.39% (84.88 - 6.49%) to 92.35% (98.86 - 6.49%) on D-7 and from 83.79% (90.28 - 6.49%) to 93.46% (99.95 - 6.49%) on D-14, respectively. On D-21, the seroconversion rate in the 55 heifers from the control group vaccinated on D-14 was 100% (55/55) (95% CI [93.51 - 100%], p < 0.05) but was not significantly different (p > 0.05) from that of heifers from the intervention group vaccinated on D-7. The results of this study allow making recommendations to the Official Veterinary Service on establishing a vaccination audit, with random collection of blood samples starting on day seven after vaccination in heifers considered vaccinated with B. abortus strain B-19 using the BAPA test as a diagnostic test and considering a minimum seroconversion rate of 75% to evaluate vaccination against brucellosis.
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spelling Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortusSoroconversão atribuível à vacinação de bezerras com a cepa B-19 de Brucella abortusBrucellosisDiagnosisMonitoringPNCEBTVaccination.BruceloseDiagnósticoMonitoramentoPNCEBTVacinação.Bovine brucellosis is transmitted by Brucella abortus and causes considerable economic losses to agribusiness. To determine the seroconversion rate attributable to vaccination of heifers with B. abortus strain B-19, 330 blood serum samples from 110 heifers aged three to eight months from five properties in the municipality of Araguaína, Tocantins, Brazil, were analyzed. On day zero (D-0), blood samples were collected, and 55 heifers from the intervention group were vaccinated. The remaining blood samples were collected on days seven (D-7), 14 (D-14), and 21 (D-21). The serum samples were analyzed using the buffered acidified plate antigen (BAPA) test. The rate of seroconversion attributable to vaccination was calculated by subtracting from the seroconversion incidence rate in the intervention group the incidence rate of the control group. On D-0, 100% (110/110) of the heifers were negative in the BAPA test (95% CI [96.70 - 100%], p < 0.05). On D-7, the seroconversion rate was 94.55% (52/55) (95% CI [84.88 - 98.84%], p < 0.05) in the intervention group and 0% (0/55) (95% CI [0.00 - 6.49%], p < 0.05) in the control group. On D-14, the rate was 98.18% (54/55) (95% CI [90.28 - 99.95%], p < 0.05) in the intervention group and 0% in the control group. Therefore, seroconversion attributable to vaccination varied from 78.39% (84.88 - 6.49%) to 92.35% (98.86 - 6.49%) on D-7 and from 83.79% (90.28 - 6.49%) to 93.46% (99.95 - 6.49%) on D-14, respectively. On D-21, the seroconversion rate in the 55 heifers from the control group vaccinated on D-14 was 100% (55/55) (95% CI [93.51 - 100%], p < 0.05) but was not significantly different (p > 0.05) from that of heifers from the intervention group vaccinated on D-7. The results of this study allow making recommendations to the Official Veterinary Service on establishing a vaccination audit, with random collection of blood samples starting on day seven after vaccination in heifers considered vaccinated with B. abortus strain B-19 using the BAPA test as a diagnostic test and considering a minimum seroconversion rate of 75% to evaluate vaccination against brucellosis.A brucelose bovina é causada por Brucella abortus gerando perdas econômicas para a cadeia do agronegócio. Objetivando determinar o coeficiente de soroconversão atribuível à vacinação de bezerras com a cepa B-19 de B. abortus, foram analisadas 330 amostras de soro sanguíneo de 110 bezerras com três a oito meses de idade, provenientes de cinco propriedades do município de Araguaína/TO. O dia zero (D-0) foi marcado pela coleta de sangue dos animais do experimento seguida da vacinação das 55 bezerras do grupo intervenção. As outras coletas de sangue foram realizadas nos dias 7 (D-7), 14 (D-14) e 21 (D-21). As amostras de soro foram submetidas ao teste do antígeno acidificado tamponado (AAT). O coeficiente de soroconversão atribuível à vacinação foi calculado subtraindo-se da incidência de soroconversão no grupo intervenção àquela do grupo controle. No D-0, 100% (110/110) das bezerras amostradas não apresentaram reação ao teste do AAT (IC 95% [96,70% a 100%]; p < 0,05). No D-7 a soroconversão no grupo intervenção foi de 94,55% (52/55) (IC 95% [84,88% a 98,84%]; p < 0,05) e no grupo controle foi nula (0/55) (IC 95% [0,00% a 6,49%]; p < 0,05). No D-14 a soroconversão no grupo controle continuou sendo nula e a do grupo intervenção foi de 98,18% (54/55) (IC 95% [90,28% a 99,95%]; p0,05) comparada à resposta no D-7 do grupo intervenção. Os resultados deste estudo permitem recomendar ao Serviço Veterinário Oficial (SVO) que institua auditoria da vacinação, com coleta aleatória de amostras de sangue a partir do sétimo dia em bezerras declaradas como vacinadas com a cepa B-19 de B. abortus, utilizando a prova do AAT como teste de diagnóstico e considerando soroconversão mínima de 75%, visando monitorar a vacinação contra brucelose.UEL2019-05-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa empírica de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3280110.5433/1679-0359.2019v40n3p1145Semina: Ciências Agrárias; Vol. 40 No. 3 (2019); 1145-1152Semina: Ciências Agrárias; v. 40 n. 3 (2019); 1145-11521679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32801/25415Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSantos, Alessandro José Ferreira dosAlmeida, Katyane de SousaBaptista, FranciscoSilva, Marco Augusto Giannoccaro daAlexandrino, BrunaFerreira, Jardel Martins2022-10-19T13:19:22Zoai:ojs.pkp.sfu.ca:article/32801Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T13:19:22Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
Soroconversão atribuível à vacinação de bezerras com a cepa B-19 de Brucella abortus
title Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
spellingShingle Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
Santos, Alessandro José Ferreira dos
Brucellosis
Diagnosis
Monitoring
PNCEBT
Vaccination.
Brucelose
Diagnóstico
Monitoramento
PNCEBT
Vacinação.
title_short Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
title_full Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
title_fullStr Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
title_full_unstemmed Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
title_sort Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus
author Santos, Alessandro José Ferreira dos
author_facet Santos, Alessandro José Ferreira dos
Almeida, Katyane de Sousa
Baptista, Francisco
Silva, Marco Augusto Giannoccaro da
Alexandrino, Bruna
Ferreira, Jardel Martins
author_role author
author2 Almeida, Katyane de Sousa
Baptista, Francisco
Silva, Marco Augusto Giannoccaro da
Alexandrino, Bruna
Ferreira, Jardel Martins
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Alessandro José Ferreira dos
Almeida, Katyane de Sousa
Baptista, Francisco
Silva, Marco Augusto Giannoccaro da
Alexandrino, Bruna
Ferreira, Jardel Martins
dc.subject.por.fl_str_mv Brucellosis
Diagnosis
Monitoring
PNCEBT
Vaccination.
Brucelose
Diagnóstico
Monitoramento
PNCEBT
Vacinação.
topic Brucellosis
Diagnosis
Monitoring
PNCEBT
Vaccination.
Brucelose
Diagnóstico
Monitoramento
PNCEBT
Vacinação.
description Bovine brucellosis is transmitted by Brucella abortus and causes considerable economic losses to agribusiness. To determine the seroconversion rate attributable to vaccination of heifers with B. abortus strain B-19, 330 blood serum samples from 110 heifers aged three to eight months from five properties in the municipality of Araguaína, Tocantins, Brazil, were analyzed. On day zero (D-0), blood samples were collected, and 55 heifers from the intervention group were vaccinated. The remaining blood samples were collected on days seven (D-7), 14 (D-14), and 21 (D-21). The serum samples were analyzed using the buffered acidified plate antigen (BAPA) test. The rate of seroconversion attributable to vaccination was calculated by subtracting from the seroconversion incidence rate in the intervention group the incidence rate of the control group. On D-0, 100% (110/110) of the heifers were negative in the BAPA test (95% CI [96.70 - 100%], p < 0.05). On D-7, the seroconversion rate was 94.55% (52/55) (95% CI [84.88 - 98.84%], p < 0.05) in the intervention group and 0% (0/55) (95% CI [0.00 - 6.49%], p < 0.05) in the control group. On D-14, the rate was 98.18% (54/55) (95% CI [90.28 - 99.95%], p < 0.05) in the intervention group and 0% in the control group. Therefore, seroconversion attributable to vaccination varied from 78.39% (84.88 - 6.49%) to 92.35% (98.86 - 6.49%) on D-7 and from 83.79% (90.28 - 6.49%) to 93.46% (99.95 - 6.49%) on D-14, respectively. On D-21, the seroconversion rate in the 55 heifers from the control group vaccinated on D-14 was 100% (55/55) (95% CI [93.51 - 100%], p < 0.05) but was not significantly different (p > 0.05) from that of heifers from the intervention group vaccinated on D-7. The results of this study allow making recommendations to the Official Veterinary Service on establishing a vaccination audit, with random collection of blood samples starting on day seven after vaccination in heifers considered vaccinated with B. abortus strain B-19 using the BAPA test as a diagnostic test and considering a minimum seroconversion rate of 75% to evaluate vaccination against brucellosis.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa empírica de campo
format article
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dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32801
10.5433/1679-0359.2019v40n3p1145
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32801
identifier_str_mv 10.5433/1679-0359.2019v40n3p1145
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32801/25415
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 3 (2019); 1145-1152
Semina: Ciências Agrárias; v. 40 n. 3 (2019); 1145-1152
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
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instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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