Primer design for complete sequencing of the Avastrovirus ORF2 gene

Detalhes bibliográficos
Autor(a) principal: Espinoza, Luis Ramiro Luna
Data de Publicação: 2018
Outros Autores: Rodrigues Beserra, Laila Andreia, Soares, Rodrigo Martins, Gregori, Fabio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25110
Resumo: Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention.
id UEL-11_43afed1c76a82f16e354aef7a70d2d1d
oai_identifier_str oai:ojs.pkp.sfu.ca:article/25110
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Primer design for complete sequencing of the Avastrovirus ORF2 geneDesenho de primers para o sequenciamento completo do gene ORF2 em AvastrovirusPCRSequencingAvastrovirusAvianORF2.PCRSequenciamentoAvastrovírusAvesORF2.Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention.As infecções por avastrovírus estão associados à doença entérica, nefrite e hepatite em aves. Aqui, nos presentamos um protocolo planejado para o sequenciamento completo do gene ORF2 em Avian Nephritis Vírus (ANV), Chicken Astrovirus (CAstV) e Turkey Astrovirus tipo 1 (TAstV-1), usando a técnica de sequenciamento convencional de Sanger. Foram usados primers previamente descritos e desenhados neste estudo, tendo como alvo as regiões conservadas flanqueadoras e internas dentro do gene ORF2 nos três vírus. O conhecimento destas sequencias é um elemento chave para caracterizar o vírus e prover de dados em diversos aspectos da epidemiologia e prevenção da doença.UEL2018-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa experimentalAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2511010.5433/1679-0359.2018v39n1p411Semina: Ciências Agrárias; Vol. 39 No. 1 (2018); 411-418Semina: Ciências Agrárias; v. 39 n. 1 (2018); 411-4181679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25110/23074Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessEspinoza, Luis Ramiro LunaRodrigues Beserra, Laila AndreiaSoares, Rodrigo MartinsGregori, Fabio2022-10-21T12:31:09Zoai:ojs.pkp.sfu.ca:article/25110Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T12:31:09Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Primer design for complete sequencing of the Avastrovirus ORF2 gene
Desenho de primers para o sequenciamento completo do gene ORF2 em Avastrovirus
title Primer design for complete sequencing of the Avastrovirus ORF2 gene
spellingShingle Primer design for complete sequencing of the Avastrovirus ORF2 gene
Espinoza, Luis Ramiro Luna
PCR
Sequencing
Avastrovirus
Avian
ORF2.
PCR
Sequenciamento
Avastrovírus
Aves
ORF2.
title_short Primer design for complete sequencing of the Avastrovirus ORF2 gene
title_full Primer design for complete sequencing of the Avastrovirus ORF2 gene
title_fullStr Primer design for complete sequencing of the Avastrovirus ORF2 gene
title_full_unstemmed Primer design for complete sequencing of the Avastrovirus ORF2 gene
title_sort Primer design for complete sequencing of the Avastrovirus ORF2 gene
author Espinoza, Luis Ramiro Luna
author_facet Espinoza, Luis Ramiro Luna
Rodrigues Beserra, Laila Andreia
Soares, Rodrigo Martins
Gregori, Fabio
author_role author
author2 Rodrigues Beserra, Laila Andreia
Soares, Rodrigo Martins
Gregori, Fabio
author2_role author
author
author
dc.contributor.author.fl_str_mv Espinoza, Luis Ramiro Luna
Rodrigues Beserra, Laila Andreia
Soares, Rodrigo Martins
Gregori, Fabio
dc.subject.por.fl_str_mv PCR
Sequencing
Avastrovirus
Avian
ORF2.
PCR
Sequenciamento
Avastrovírus
Aves
ORF2.
topic PCR
Sequencing
Avastrovirus
Avian
ORF2.
PCR
Sequenciamento
Avastrovírus
Aves
ORF2.
description Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa experimental
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25110
10.5433/1679-0359.2018v39n1p411
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25110
identifier_str_mv 10.5433/1679-0359.2018v39n1p411
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25110/23074
dc.rights.driver.fl_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 39 No. 1 (2018); 411-418
Semina: Ciências Agrárias; v. 39 n. 1 (2018); 411-418
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1750315423863144448

Registros relacionados