Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)

Detalhes bibliográficos
Autor(a) principal: Fornari, Darci Carlos
Data de Publicação: 2014
Outros Autores: Streit Jr, Danilo Pedro, Tonissi, Laura Botta, Povh, Jayme Aparecido, Sirol, Rodolfo Nardez, Ribeiro, Ricardo Pereira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432
Resumo: The aim of this study was to evaluate cryoprotectants for cooling embryos of B. orbignyanus. 1400 embryos were selected, in the post-gastrulation and subjected to cooling to -8°C with different combinations of intracellular cryoprotectants (methanol and 9% DMSO or associated 4.5%) combined with extracellular cryoprotectants (sucrose and glucose, 17%) diluted in distilled water to complete 100%. For each treatment, 200 embryos were placed in glass tubes and submitted to the cooling process. After the proposed time, the embryos were removed and transferred to four-liter incubation to hatching of the larvae. The results obtained from solutions containing glucose were inefficient. The treatments with association of DMSO showed no satisfactory results. For cooling of B. orbignyanus embryos at -8°C it is suggested to use a cryoprotectant solution containing 17% sucrose associated with 9% methanol. 
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spelling Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)Crioprotetores para o resfriamento de embriões de piracanjuba Brycon orbignyanus (Valenciennes, 1850)BiotechnologyCryopreservationCryoprotectantMethanolSucrose.BiotecnologiaCriopreservaçãoCrioprotetorMetanolSacarose.Aquiculturareprodução animalThe aim of this study was to evaluate cryoprotectants for cooling embryos of B. orbignyanus. 1400 embryos were selected, in the post-gastrulation and subjected to cooling to -8°C with different combinations of intracellular cryoprotectants (methanol and 9% DMSO or associated 4.5%) combined with extracellular cryoprotectants (sucrose and glucose, 17%) diluted in distilled water to complete 100%. For each treatment, 200 embryos were placed in glass tubes and submitted to the cooling process. After the proposed time, the embryos were removed and transferred to four-liter incubation to hatching of the larvae. The results obtained from solutions containing glucose were inefficient. The treatments with association of DMSO showed no satisfactory results. For cooling of B. orbignyanus embryos at -8°C it is suggested to use a cryoprotectant solution containing 17% sucrose associated with 9% methanol. O objetivo deste trabalho foi avaliar a eficiência dos crioprotetores para o resfriamento de embriões de B. orbignyanus. Foram selecionados 1.400 embriões viáveis, na fase de pós-gastrulação e submetidos ao resfriamento a -8ºC com diferentes combinações de crioprotetores permeáveis (Metanol e dimetilsulfóxido - DMSO 9% ou associados 4,5%) combinados à crioprotetores não permeáveis (Sacarose e Glicose, 17%). Para cada tratamento, 200 embriões foram alocados em tubos de vidro “vacutainer” e submetidos ao processo de resfriamento. Após o tempo proposto, os embriões foram retirados e transferidos para incubadoras de quatro litros até a eclosão das larvas. Os resultados com as soluções contendo glicose no resfriamento foram ineficiente, apenas 27% dos embriões sobreviveram no tratamento. Os tratamentos com associação do DMSO não houve sobrevivência. Para o resfriamento de embriões de B. orbignyanus exposto a -8°C por um período de seis horas deve-se utilizar solução crioprotetora contendo sacarose 17% associada ao metanol 9%.UEL2014-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRevisão de literaturaapplication/pdfimage/jpegapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1543210.5433/1679-0359.2014v35n4Suplp2809Semina: Ciências Agrárias; Vol. 35 No. 4Supl (2014); 2809-2816Semina: Ciências Agrárias; v. 35 n. 4Supl (2014); 2809-28161679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432/pdf_457https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432/33665https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432/34423Fornari, Darci CarlosStreit Jr, Danilo PedroTonissi, Laura BottaPovh, Jayme AparecidoSirol, Rodolfo NardezRibeiro, Ricardo Pereirainfo:eu-repo/semantics/openAccess2015-11-19T18:35:40Zoai:ojs.pkp.sfu.ca:article/15432Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:35:40Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
Crioprotetores para o resfriamento de embriões de piracanjuba Brycon orbignyanus (Valenciennes, 1850)
title Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
spellingShingle Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
Fornari, Darci Carlos
Biotechnology
Cryopreservation
Cryoprotectant
Methanol
Sucrose.
Biotecnologia
Criopreservação
Crioprotetor
Metanol
Sacarose.
Aquicultura
reprodução animal
title_short Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
title_full Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
title_fullStr Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
title_full_unstemmed Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
title_sort Cooling protocol for embryos of piracanjuba Brycon orbignyanus (Valenciennes, 1850)
author Fornari, Darci Carlos
author_facet Fornari, Darci Carlos
Streit Jr, Danilo Pedro
Tonissi, Laura Botta
Povh, Jayme Aparecido
Sirol, Rodolfo Nardez
Ribeiro, Ricardo Pereira
author_role author
author2 Streit Jr, Danilo Pedro
Tonissi, Laura Botta
Povh, Jayme Aparecido
Sirol, Rodolfo Nardez
Ribeiro, Ricardo Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fornari, Darci Carlos
Streit Jr, Danilo Pedro
Tonissi, Laura Botta
Povh, Jayme Aparecido
Sirol, Rodolfo Nardez
Ribeiro, Ricardo Pereira
dc.subject.por.fl_str_mv Biotechnology
Cryopreservation
Cryoprotectant
Methanol
Sucrose.
Biotecnologia
Criopreservação
Crioprotetor
Metanol
Sacarose.
Aquicultura
reprodução animal
topic Biotechnology
Cryopreservation
Cryoprotectant
Methanol
Sucrose.
Biotecnologia
Criopreservação
Crioprotetor
Metanol
Sacarose.
Aquicultura
reprodução animal
description The aim of this study was to evaluate cryoprotectants for cooling embryos of B. orbignyanus. 1400 embryos were selected, in the post-gastrulation and subjected to cooling to -8°C with different combinations of intracellular cryoprotectants (methanol and 9% DMSO or associated 4.5%) combined with extracellular cryoprotectants (sucrose and glucose, 17%) diluted in distilled water to complete 100%. For each treatment, 200 embryos were placed in glass tubes and submitted to the cooling process. After the proposed time, the embryos were removed and transferred to four-liter incubation to hatching of the larvae. The results obtained from solutions containing glucose were inefficient. The treatments with association of DMSO showed no satisfactory results. For cooling of B. orbignyanus embryos at -8°C it is suggested to use a cryoprotectant solution containing 17% sucrose associated with 9% methanol. 
publishDate 2014
dc.date.none.fl_str_mv 2014-09-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Revisão de literatura
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432
10.5433/1679-0359.2014v35n4Suplp2809
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432
identifier_str_mv 10.5433/1679-0359.2014v35n4Suplp2809
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432/pdf_457
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432/33665
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15432/34423
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
image/jpeg
application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 35 No. 4Supl (2014); 2809-2816
Semina: Ciências Agrárias; v. 35 n. 4Supl (2014); 2809-2816
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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