Effect of ozonation on the Staphylococcus Aureus innoculated in milk
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21805 |
Resumo: | The aim of this research was evaluate the efficiency of the action of ozonation of milk on the reduction of the bacterial counts of Staphylococcus aureus artificially inoculated and subjected to different binomials times/concentrations of ozone. The samples were divided in four assays: 1 and 2, with skim milk and ozone concentrations of 34.7 mg L –1 and 44.8 mg L-1, respectively, and assays 3 and 4, with whole milk and the same concentrations of ozone. The time of ozonation in all of the assays was 5 (T1), 10 (T2), 15 (T3), 20 (T4) and 25 (T5) minutes. Each treatment was replicated three times. The bacterial counts were determined immediately before and after the ozonation process by plating the samples on Baird Parker agar. In treatments 1 and 2, the greatest reductions were 0.42 log10 CFU/mL (colony forming units per millilitre), whereas in treatments 3 and 4, the greatest reductions were 0.19 and 0.21 log10 CFU/mL, respectively. The results of all of the tests show that, for T1, there was no reduction in the microbial load, whereas for T2, there was a sharp decrease in the microbiota, and this decrease was even more significant for T4 and T5. In both skim and whole milk with ozone concentrations of 34.7 and 44.8 mg L-1, the reductions were significantly greater after 20 minutes of ozonation. The results indicated that ozone showed low efficacy in the reduction of S.aureus inoculated into fluid milk and milk fat interferes with the bactericidal action of the gas. |
id |
UEL-11_7cd1fb969c051cf3c609a27959de2cd0 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/21805 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Effect of ozonation on the Staphylococcus Aureus innoculated in milkEfeito da ozonização sobre a contagem da Staphylococcus Aureus inoculado em leiteOzoneStaphylococcus aureusMilkMilk processing.OzônioStaphylococcus aureusLeiteBeneficiamento do leite.The aim of this research was evaluate the efficiency of the action of ozonation of milk on the reduction of the bacterial counts of Staphylococcus aureus artificially inoculated and subjected to different binomials times/concentrations of ozone. The samples were divided in four assays: 1 and 2, with skim milk and ozone concentrations of 34.7 mg L –1 and 44.8 mg L-1, respectively, and assays 3 and 4, with whole milk and the same concentrations of ozone. The time of ozonation in all of the assays was 5 (T1), 10 (T2), 15 (T3), 20 (T4) and 25 (T5) minutes. Each treatment was replicated three times. The bacterial counts were determined immediately before and after the ozonation process by plating the samples on Baird Parker agar. In treatments 1 and 2, the greatest reductions were 0.42 log10 CFU/mL (colony forming units per millilitre), whereas in treatments 3 and 4, the greatest reductions were 0.19 and 0.21 log10 CFU/mL, respectively. The results of all of the tests show that, for T1, there was no reduction in the microbial load, whereas for T2, there was a sharp decrease in the microbiota, and this decrease was even more significant for T4 and T5. In both skim and whole milk with ozone concentrations of 34.7 and 44.8 mg L-1, the reductions were significantly greater after 20 minutes of ozonation. The results indicated that ozone showed low efficacy in the reduction of S.aureus inoculated into fluid milk and milk fat interferes with the bactericidal action of the gas.O objetivo dessa pesquisa foi avaliar a eficiência do ozônio sobre a redução da contagem de Staphylococcus aureus artificialmente inoculados em leite, utilizando-se diferentes binômios tempo/ concentração. Foram realizados quatro ensaios: 1 e 2, com leite desnatado e concentrações de 34,7 mg L-1 e 44,8 mg L-1 de ozônio, respectivamente; e ensaios 3 e 4, com leite integral nas mesmas concentrações de ozônio. Os tempos de ozonização em todos os ensaios foram de 5 (T1), 10 (T2), 15 (T3), 20 (T4) e 25 (T5) minutos, sendo cada tratamento realizado em triplicata. As contagens bacterianas foram feitas imediatamente antes e após o processo de ozonização das amostras, em agar Baird Parker. Nos tratamentos 1 e 2, as maiores reduções foram de 0,42 log10 UFC/mL, enquanto nos tratamentos 3 e 4, foram de 0,19 e 0,21 log10 UFC/mL, respectivamente. Os resultados de todos os ensaios mostraram que, em T1, não houve redução da carga microbiana, enquanto que a partir de T2, ocorreu uma redução crescente nas contagens, que foi acentuada nos tempos T4 e T5. Tanto para leite desnatado quanto para integral, nas concentrações de 34,7 e 44,8 mg L-1, a partir dos 20 minutos de ozonização, as reduções foram significativamente maiores. Os resultados indicaram que o ozônio apresentou baixa eficácia na redução de S. aureus inoculado em leite fluido e que a gordura do leite interfere na ação bactericida do gás.UEL2016-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2180510.5433/1679-0359.2016v37n4p1911Semina: Ciências Agrárias; Vol. 37 No. 4 (2016); 1911-1918Semina: Ciências Agrárias; v. 37 n. 4 (2016); 1911-19181679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21805/19637http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCouto, Emanuel PereiraAlencar, Ernandes RodriguesGonçalves, Vítor Salvador PicãoSantos, Anderson Joaquim Pereira dosRibeiro, Jaqueline LamounierFerreira, Márcia de Aguiar2022-12-01T11:38:42Zoai:ojs.pkp.sfu.ca:article/21805Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-01T11:38:42Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk Efeito da ozonização sobre a contagem da Staphylococcus Aureus inoculado em leite |
title |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk |
spellingShingle |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk Couto, Emanuel Pereira Ozone Staphylococcus aureus Milk Milk processing. Ozônio Staphylococcus aureus Leite Beneficiamento do leite. |
title_short |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk |
title_full |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk |
title_fullStr |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk |
title_full_unstemmed |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk |
title_sort |
Effect of ozonation on the Staphylococcus Aureus innoculated in milk |
author |
Couto, Emanuel Pereira |
author_facet |
Couto, Emanuel Pereira Alencar, Ernandes Rodrigues Gonçalves, Vítor Salvador Picão Santos, Anderson Joaquim Pereira dos Ribeiro, Jaqueline Lamounier Ferreira, Márcia de Aguiar |
author_role |
author |
author2 |
Alencar, Ernandes Rodrigues Gonçalves, Vítor Salvador Picão Santos, Anderson Joaquim Pereira dos Ribeiro, Jaqueline Lamounier Ferreira, Márcia de Aguiar |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Couto, Emanuel Pereira Alencar, Ernandes Rodrigues Gonçalves, Vítor Salvador Picão Santos, Anderson Joaquim Pereira dos Ribeiro, Jaqueline Lamounier Ferreira, Márcia de Aguiar |
dc.subject.por.fl_str_mv |
Ozone Staphylococcus aureus Milk Milk processing. Ozônio Staphylococcus aureus Leite Beneficiamento do leite. |
topic |
Ozone Staphylococcus aureus Milk Milk processing. Ozônio Staphylococcus aureus Leite Beneficiamento do leite. |
description |
The aim of this research was evaluate the efficiency of the action of ozonation of milk on the reduction of the bacterial counts of Staphylococcus aureus artificially inoculated and subjected to different binomials times/concentrations of ozone. The samples were divided in four assays: 1 and 2, with skim milk and ozone concentrations of 34.7 mg L –1 and 44.8 mg L-1, respectively, and assays 3 and 4, with whole milk and the same concentrations of ozone. The time of ozonation in all of the assays was 5 (T1), 10 (T2), 15 (T3), 20 (T4) and 25 (T5) minutes. Each treatment was replicated three times. The bacterial counts were determined immediately before and after the ozonation process by plating the samples on Baird Parker agar. In treatments 1 and 2, the greatest reductions were 0.42 log10 CFU/mL (colony forming units per millilitre), whereas in treatments 3 and 4, the greatest reductions were 0.19 and 0.21 log10 CFU/mL, respectively. The results of all of the tests show that, for T1, there was no reduction in the microbial load, whereas for T2, there was a sharp decrease in the microbiota, and this decrease was even more significant for T4 and T5. In both skim and whole milk with ozone concentrations of 34.7 and 44.8 mg L-1, the reductions were significantly greater after 20 minutes of ozonation. The results indicated that ozone showed low efficacy in the reduction of S.aureus inoculated into fluid milk and milk fat interferes with the bactericidal action of the gas. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21805 10.5433/1679-0359.2016v37n4p1911 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21805 |
identifier_str_mv |
10.5433/1679-0359.2016v37n4p1911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21805/19637 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 4 (2016); 1911-1918 Semina: Ciências Agrárias; v. 37 n. 4 (2016); 1911-1918 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306074030866432 |