Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/17889 |
Resumo: | The entomopathogenic fungus Beauveria bassiana is a promising agent for use in insect control. Its pathogenic activity, as well as other factors such as temperature that can interfere with its development, should be assessed, thus, establishing the foundations for B. bassiana use in biological control programs. The objective of this study was to select and induce tolerance of B. bassiana isolates to high and low temperatures and to assess their virulence before and after exposure to those temperatures. A pre-selection test was performed, in which the tolerance of isolates to stress temperatures was tested and compared to the ideal growth temperature of 25 °C for this organism. For the isolates/temperature combinations resulting in growth, conidia germination and colony-forming units (CFUs) were assessed. The isolates Unioeste 4 and Unioeste 40 exhibited >95% germinated conidia at 16 and 31 °C. Thereafter, they underwent four consecutive passages at maximum and minimum tolerated temperatures (10 and 37 °C). A significant difference in germination was observed between the two isolates at all temperatures tested. More CFUs were observed for Unioeste 4 compared to Unioeste 40 at all temperatures, and in the case of the latter, there was no difference in CFU formation at 10 and 25 °C. For both isolates, decreased vegetative growth was observed at 37 °C. Recovery of virulence was observed in both isolates, as determined by insect mortality. No relationship was observed between production of the enzyme Pr1 and the virulence of the isolates. |
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Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperaturesSeleção, avaliação da virulência a Alphitobius diaperinus e produção da enzima Pr1 de isolados de Beauveria bassiana (Bals.) Vuill. submetidos a temperaturas de estresseEntomopathogenic fungusLesser mealwormAviaryProtease.Fungo entomopatogênicoCascudinhoAviárioProtease.Controle BiológicoThe entomopathogenic fungus Beauveria bassiana is a promising agent for use in insect control. Its pathogenic activity, as well as other factors such as temperature that can interfere with its development, should be assessed, thus, establishing the foundations for B. bassiana use in biological control programs. The objective of this study was to select and induce tolerance of B. bassiana isolates to high and low temperatures and to assess their virulence before and after exposure to those temperatures. A pre-selection test was performed, in which the tolerance of isolates to stress temperatures was tested and compared to the ideal growth temperature of 25 °C for this organism. For the isolates/temperature combinations resulting in growth, conidia germination and colony-forming units (CFUs) were assessed. The isolates Unioeste 4 and Unioeste 40 exhibited >95% germinated conidia at 16 and 31 °C. Thereafter, they underwent four consecutive passages at maximum and minimum tolerated temperatures (10 and 37 °C). A significant difference in germination was observed between the two isolates at all temperatures tested. More CFUs were observed for Unioeste 4 compared to Unioeste 40 at all temperatures, and in the case of the latter, there was no difference in CFU formation at 10 and 25 °C. For both isolates, decreased vegetative growth was observed at 37 °C. Recovery of virulence was observed in both isolates, as determined by insect mortality. No relationship was observed between production of the enzyme Pr1 and the virulence of the isolates.O fungo entomopatogênico Beauveria bassiana é relatado como um promissor agente de controle de insetos. É necessário investigar a atividade patogênica e também fatores como a temperatura que pode interferir no seu desenvolvimento, estabelecendo assim bases para o seu uso em programas de controle biológico. O objetivo desse trabalho foi selecionar e induzir a tolerância a temperaturas baixas e elevadas de B. bassiana e verificar a virulência desses antes e após a exposição às temperaturas. Realizou-se uma pré-seleção, onde foi verificada a tolerância dos isolados as temperaturas de estresse, os testes foram comparados à temperatura de 25 °C ideal para o crescimento desse microrganismo. Para os isolados/temperaturas onde houve crescimento, foi avaliada a germinação de conídios e unidades formadoras de colônias. Os isolados Unioeste 4 e Unioeste 40 apresentaram mais de 95% de conídios germinados nas temperaturas de 16 °C e 31 °C. Em seguida para esses mesmos isolados foram feitas quatro repicagens consecutivas nas temperaturas máxima e mínima tolerada (10 °C e 37 °C). Para a germinação foi observada diferença significativa entre os dois isolados em todas as temperaturas. Foi observada a maior formação de UFC para o isolado Unioeste 4 quando comparado com o Unioeste 40 em todas as temperaturas. Para esse mesmo isolado não houve diferença entre as temperaturas de 10 °C e 25 °C. Para ambos os isolados o menor crescimento vegetativo foi constatado a 37 °C. Foi possível verificar uma recuperação na virulência dos dois isolados na mortalidade dos insetos. Não foi observada relação na produção da enzima Pr1 com a virulência dos isolados.UEL2015-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1788910.5433/1679-0359.2015v36n6p3529Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3529-3538Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3529-35381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/17889/17436http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessConstanski, Kelly ChristianeZorzetti, JanainaNeves, Pedro Manuel Oliveira Janeiro2022-12-02T16:07:36Zoai:ojs.pkp.sfu.ca:article/17889Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T16:07:36Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures Seleção, avaliação da virulência a Alphitobius diaperinus e produção da enzima Pr1 de isolados de Beauveria bassiana (Bals.) Vuill. submetidos a temperaturas de estresse |
title |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures |
spellingShingle |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures Constanski, Kelly Christiane Entomopathogenic fungus Lesser mealworm Aviary Protease. Fungo entomopatogênico Cascudinho Aviário Protease. Controle Biológico |
title_short |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures |
title_full |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures |
title_fullStr |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures |
title_full_unstemmed |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures |
title_sort |
Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures |
author |
Constanski, Kelly Christiane |
author_facet |
Constanski, Kelly Christiane Zorzetti, Janaina Neves, Pedro Manuel Oliveira Janeiro |
author_role |
author |
author2 |
Zorzetti, Janaina Neves, Pedro Manuel Oliveira Janeiro |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Constanski, Kelly Christiane Zorzetti, Janaina Neves, Pedro Manuel Oliveira Janeiro |
dc.subject.por.fl_str_mv |
Entomopathogenic fungus Lesser mealworm Aviary Protease. Fungo entomopatogênico Cascudinho Aviário Protease. Controle Biológico |
topic |
Entomopathogenic fungus Lesser mealworm Aviary Protease. Fungo entomopatogênico Cascudinho Aviário Protease. Controle Biológico |
description |
The entomopathogenic fungus Beauveria bassiana is a promising agent for use in insect control. Its pathogenic activity, as well as other factors such as temperature that can interfere with its development, should be assessed, thus, establishing the foundations for B. bassiana use in biological control programs. The objective of this study was to select and induce tolerance of B. bassiana isolates to high and low temperatures and to assess their virulence before and after exposure to those temperatures. A pre-selection test was performed, in which the tolerance of isolates to stress temperatures was tested and compared to the ideal growth temperature of 25 °C for this organism. For the isolates/temperature combinations resulting in growth, conidia germination and colony-forming units (CFUs) were assessed. The isolates Unioeste 4 and Unioeste 40 exhibited >95% germinated conidia at 16 and 31 °C. Thereafter, they underwent four consecutive passages at maximum and minimum tolerated temperatures (10 and 37 °C). A significant difference in germination was observed between the two isolates at all temperatures tested. More CFUs were observed for Unioeste 4 compared to Unioeste 40 at all temperatures, and in the case of the latter, there was no difference in CFU formation at 10 and 25 °C. For both isolates, decreased vegetative growth was observed at 37 °C. Recovery of virulence was observed in both isolates, as determined by insect mortality. No relationship was observed between production of the enzyme Pr1 and the virulence of the isolates. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/17889 10.5433/1679-0359.2015v36n6p3529 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/17889 |
identifier_str_mv |
10.5433/1679-0359.2015v36n6p3529 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/17889/17436 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3529-3538 Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3529-3538 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306071995580416 |