Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7003 |
Resumo: | Disruptions in sperm production include decreased sperm motility and increased of abnormal sperm. Seminal plasma appears to exert important effects on sperm function. The objective was to evaluate the dry and rainy season influence on the seminal characteristics and semen plasma proteins. Eleven bulls with ages ranging from 34 to 38 months were submitted each one to 12 semen collect with eletroejaculation 6 on dry season and 6 on rainy season with 14 days interval, totalizing 144 samples. Qualitative and quantitative semen characteristics were evaluated. Samples of semen were centrifuged (1.500 g / 15 minutes) and conditioned and stored (–20ºC) until further processing. The proteins were extracted and quantified to electrophoresis performed. Variance analysis and Tukey test 5% was used. The semen vigor (P < 0.01), minor defects and total defects (P < 0.05) showed statistical difference between seasons, while the volume, motility and minor defects did not (P > 0.05). The number of bands occurred between 6 and 125 kDa. The molecular band of 26 kDa was present in 100% of bulls in rainy season. The molecular bands of 6, 9 and 125 kDa showed a high frequency in dry and rainy season. In conclusion, these results showed a band distribution variation throughout the season and the year seasons changed the semen quality with increase sperm vigor and reduction of abnormal sperm on dry season. |
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Semina. Ciências Agrárias (Online) |
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Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicusInfluência das estações seca e chuvosa no plasma seminal (SDS-PAGE) e características do ejaculado de touros Bos taurus indicusZebu bullsSeasonSêmenSeminal plasmaElectrophoresis.Touro Bos taurus indicusEstaçãoSêmenPlasma seminalEletroforese.5.05.04.01-0 ginecologia e andrologia animalDisruptions in sperm production include decreased sperm motility and increased of abnormal sperm. Seminal plasma appears to exert important effects on sperm function. The objective was to evaluate the dry and rainy season influence on the seminal characteristics and semen plasma proteins. Eleven bulls with ages ranging from 34 to 38 months were submitted each one to 12 semen collect with eletroejaculation 6 on dry season and 6 on rainy season with 14 days interval, totalizing 144 samples. Qualitative and quantitative semen characteristics were evaluated. Samples of semen were centrifuged (1.500 g / 15 minutes) and conditioned and stored (–20ºC) until further processing. The proteins were extracted and quantified to electrophoresis performed. Variance analysis and Tukey test 5% was used. The semen vigor (P < 0.01), minor defects and total defects (P < 0.05) showed statistical difference between seasons, while the volume, motility and minor defects did not (P > 0.05). The number of bands occurred between 6 and 125 kDa. The molecular band of 26 kDa was present in 100% of bulls in rainy season. The molecular bands of 6, 9 and 125 kDa showed a high frequency in dry and rainy season. In conclusion, these results showed a band distribution variation throughout the season and the year seasons changed the semen quality with increase sperm vigor and reduction of abnormal sperm on dry season.O desequilíbrio na espermatogênese inclui diminuição na motilidade e aumento de espermatozóides alterados. O plasma seminal exerce efeito na função espermática. O objetivo foi avaliar a influência das estações seca e chuvosa nas características do sêmen e proteínas do plasma seminal. Onze touros Bos taurus indicus com 34 a 38 meses de idade foram submetidos cada um a 12 colheitas de sêmen com eletroejaculador, 6 na estação seca e 6 na chuvosa, a cada 14 dias, totalizando 144 amostras. Características qualitativas e quantitativas foram avaliadas, sendo as amostras centrifugadas (1.500 g / 15 min) e estocadas (-20ºC). As proteínas foram extraídas e quantificadas para as eletroforeses. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5%. Houve diferença entre estações para o vigor (P < 0,01), defeitos menores e totais (P < 0,05); e não para o volume, motilidade e defeitos maiores. A ocorrência do número de bandas esteve entre 6 e 125 kDa. A banda de 26 kDa esteve presente em 100% dos touros na estação chuvosa. As bandas de 6, 9 e 125 kDa mostraram alta freqüência nas estações seca e chuvosa. Conclui-se que os resultados demonstraram variação na distribuição de bandas nas estações seca e chuvosa; e que as estações do ano alteram a qualidade do sêmen com melhoria do vigor e redução das patologias espermáticas na estação seca.UEL2011-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/700310.5433/1679-0359.2011v32n4p1565Semina: Ciências Agrárias; Vol. 32 No. 4 (2011); 1565-1574Semina: Ciências Agrárias; v. 32 n. 4 (2011); 1565-15741679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7003/9036Chacur, Marcelo George MungaiCastillo, Andrea Marisol NovoaGuaberto, Luciana Machadoinfo:eu-repo/semantics/openAccess2015-11-19T18:37:49Zoai:ojs.pkp.sfu.ca:article/7003Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus Influência das estações seca e chuvosa no plasma seminal (SDS-PAGE) e características do ejaculado de touros Bos taurus indicus |
| title |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus |
| spellingShingle |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus Chacur, Marcelo George Mungai Zebu bulls Season Sêmen Seminal plasma Electrophoresis. Touro Bos taurus indicus Estação Sêmen Plasma seminal Eletroforese. 5.05.04.01-0 ginecologia e andrologia animal |
| title_short |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus |
| title_full |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus |
| title_fullStr |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus |
| title_full_unstemmed |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus |
| title_sort |
Influence of the dry and rainy seasons upon plasma seminal (SDS-PAGE) and characteristics of the ejaculate from bulls Bos taurus indicus |
| author |
Chacur, Marcelo George Mungai |
| author_facet |
Chacur, Marcelo George Mungai Castillo, Andrea Marisol Novoa Guaberto, Luciana Machado |
| author_role |
author |
| author2 |
Castillo, Andrea Marisol Novoa Guaberto, Luciana Machado |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Chacur, Marcelo George Mungai Castillo, Andrea Marisol Novoa Guaberto, Luciana Machado |
| dc.subject.por.fl_str_mv |
Zebu bulls Season Sêmen Seminal plasma Electrophoresis. Touro Bos taurus indicus Estação Sêmen Plasma seminal Eletroforese. 5.05.04.01-0 ginecologia e andrologia animal |
| topic |
Zebu bulls Season Sêmen Seminal plasma Electrophoresis. Touro Bos taurus indicus Estação Sêmen Plasma seminal Eletroforese. 5.05.04.01-0 ginecologia e andrologia animal |
| description |
Disruptions in sperm production include decreased sperm motility and increased of abnormal sperm. Seminal plasma appears to exert important effects on sperm function. The objective was to evaluate the dry and rainy season influence on the seminal characteristics and semen plasma proteins. Eleven bulls with ages ranging from 34 to 38 months were submitted each one to 12 semen collect with eletroejaculation 6 on dry season and 6 on rainy season with 14 days interval, totalizing 144 samples. Qualitative and quantitative semen characteristics were evaluated. Samples of semen were centrifuged (1.500 g / 15 minutes) and conditioned and stored (–20ºC) until further processing. The proteins were extracted and quantified to electrophoresis performed. Variance analysis and Tukey test 5% was used. The semen vigor (P < 0.01), minor defects and total defects (P < 0.05) showed statistical difference between seasons, while the volume, motility and minor defects did not (P > 0.05). The number of bands occurred between 6 and 125 kDa. The molecular band of 26 kDa was present in 100% of bulls in rainy season. The molecular bands of 6, 9 and 125 kDa showed a high frequency in dry and rainy season. In conclusion, these results showed a band distribution variation throughout the season and the year seasons changed the semen quality with increase sperm vigor and reduction of abnormal sperm on dry season. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-10-14 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7003 10.5433/1679-0359.2011v32n4p1565 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7003 |
| identifier_str_mv |
10.5433/1679-0359.2011v32n4p1565 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7003/9036 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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UEL |
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UEL |
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Semina: Ciências Agrárias; Vol. 32 No. 4 (2011); 1565-1574 Semina: Ciências Agrárias; v. 32 n. 4 (2011); 1565-1574 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
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Universidade Estadual de Londrina (UEL) |
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UEL |
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UEL |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
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semina.agrarias@uel.br |
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1750315412715732992 |