Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/6128 |
Resumo: | There are no efficient fixatives for histological analyses of ovarian tissue that permit an appropriate morphological analysis of ovary and their cells. The correct processing of the tissue is essential to avoid misinterpretation between follicular degeneration due to the physiological process of follicular atresia with signs of degeneration signals due to poor tissue fixation. This study aimed to identify the best fixative and better fixation period for morphological analysis of the functional unit of the ovary: follicle. Ovaries (n = 5) from five adult cows were collected from local abattoirs. Each ovary was divided into 12 fragments and submitted to six different fixatives in two different times (12 and 24h). It was analyzed 150 follicles per treatment. The best results were obtained with Carnoy’ and Bouin’s fixatives which had higher (P < 0.05) proportion of viable follicles after fixation for 12 or 24h (88 and 83% for Carnoy’s fixative vs. 79 e 73% for Bouin). It was concluded that Carnoy and Bouin fixatives are the best options to preserve for up to 24 h the morphology of preantral follicles in bovine ovaries. |
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Semina. Ciências Agrárias (Online) |
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Effect of fixative type and fixation time on the morphology of bovine preantral ovarian folliclesEfeito do tipo de fixador e tempo de fixação na morfologia de folículos pré-antrais ovarianos bovinosFixativeHistologyOvaryPreantral follicle.FixadorHistologiaOvárioFolículo pré-antral.There are no efficient fixatives for histological analyses of ovarian tissue that permit an appropriate morphological analysis of ovary and their cells. The correct processing of the tissue is essential to avoid misinterpretation between follicular degeneration due to the physiological process of follicular atresia with signs of degeneration signals due to poor tissue fixation. This study aimed to identify the best fixative and better fixation period for morphological analysis of the functional unit of the ovary: follicle. Ovaries (n = 5) from five adult cows were collected from local abattoirs. Each ovary was divided into 12 fragments and submitted to six different fixatives in two different times (12 and 24h). It was analyzed 150 follicles per treatment. The best results were obtained with Carnoy’ and Bouin’s fixatives which had higher (P < 0.05) proportion of viable follicles after fixation for 12 or 24h (88 and 83% for Carnoy’s fixative vs. 79 e 73% for Bouin). It was concluded that Carnoy and Bouin fixatives are the best options to preserve for up to 24 h the morphology of preantral follicles in bovine ovaries.Na rotina de avaliação histológica, os fixadores mais adequados para o tecido ovariano para que se obtenha uma adequada análise morfológica do ovário e de suas células ainda não foram determinados. O correto processamento do tecido é fundamental para evitar erros de interpretação, como confundir degeneração folicular devido ao processo fisiológico de atresia folicular com sinais de degeneração devido à má fixação do tecido. Este estudo teve como objetivo identificar qual o melhor fixador e melhor período de fixação para análise morfológica da unidade funcional do ovário: o folículo. Para isso foram utilizados ovários (n=5) de cinco vacas adultas. Cada ovário foi divido em 12 fragmentos e submetidos a seis diferentes fixadores em dois tempos (12 e 24h). Foram analisados 150 folículos ovarianos por tratamento. Os melhores resultados foram obtidos com os fixadores Carnoy e Bouin, que apresentaram maior (P < 0,05) proporção de folículos viáveis após fixação por 12 ou 24h (88 e 83% para o fixador Carnoy vs. 79 e 73% para Bouin respectivamente). Conclui-se neste estudo que os fixadores Carnoy e Bouin são as melhores opções para preservar por até 24 h a morfologia de folículos pré-antrais em ovários bovinos.UEL2012-04-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa científicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/612810.5433/1679-0359.2012v33n1p297Semina: Ciências Agrárias; Vol. 33 No. 1 (2012); 297-304Semina: Ciências Agrárias; v. 33 n. 1 (2012); 297-3041679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/6128/10155Santos, Joabel Tonellotto dosSilva-Santos, Katia CristinaAndrade, Evelyn RabeloLisboa, Livia AiresSchneider, Carine LetíciaCiquini, AlethéiaFerreira, RogerioNobrega Júnior, Janduí EscariaoSeneda, Marcelo Marcondesinfo:eu-repo/semantics/openAccess2015-11-19T18:37:41Zoai:ojs.pkp.sfu.ca:article/6128Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:41Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles Efeito do tipo de fixador e tempo de fixação na morfologia de folículos pré-antrais ovarianos bovinos |
title |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles |
spellingShingle |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles Santos, Joabel Tonellotto dos Fixative Histology Ovary Preantral follicle. Fixador Histologia Ovário Folículo pré-antral. |
title_short |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles |
title_full |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles |
title_fullStr |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles |
title_full_unstemmed |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles |
title_sort |
Effect of fixative type and fixation time on the morphology of bovine preantral ovarian follicles |
author |
Santos, Joabel Tonellotto dos |
author_facet |
Santos, Joabel Tonellotto dos Silva-Santos, Katia Cristina Andrade, Evelyn Rabelo Lisboa, Livia Aires Schneider, Carine Letícia Ciquini, Alethéia Ferreira, Rogerio Nobrega Júnior, Janduí Escariao Seneda, Marcelo Marcondes |
author_role |
author |
author2 |
Silva-Santos, Katia Cristina Andrade, Evelyn Rabelo Lisboa, Livia Aires Schneider, Carine Letícia Ciquini, Alethéia Ferreira, Rogerio Nobrega Júnior, Janduí Escariao Seneda, Marcelo Marcondes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Joabel Tonellotto dos Silva-Santos, Katia Cristina Andrade, Evelyn Rabelo Lisboa, Livia Aires Schneider, Carine Letícia Ciquini, Alethéia Ferreira, Rogerio Nobrega Júnior, Janduí Escariao Seneda, Marcelo Marcondes |
dc.subject.por.fl_str_mv |
Fixative Histology Ovary Preantral follicle. Fixador Histologia Ovário Folículo pré-antral. |
topic |
Fixative Histology Ovary Preantral follicle. Fixador Histologia Ovário Folículo pré-antral. |
description |
There are no efficient fixatives for histological analyses of ovarian tissue that permit an appropriate morphological analysis of ovary and their cells. The correct processing of the tissue is essential to avoid misinterpretation between follicular degeneration due to the physiological process of follicular atresia with signs of degeneration signals due to poor tissue fixation. This study aimed to identify the best fixative and better fixation period for morphological analysis of the functional unit of the ovary: follicle. Ovaries (n = 5) from five adult cows were collected from local abattoirs. Each ovary was divided into 12 fragments and submitted to six different fixatives in two different times (12 and 24h). It was analyzed 150 follicles per treatment. The best results were obtained with Carnoy’ and Bouin’s fixatives which had higher (P < 0.05) proportion of viable follicles after fixation for 12 or 24h (88 and 83% for Carnoy’s fixative vs. 79 e 73% for Bouin). It was concluded that Carnoy and Bouin fixatives are the best options to preserve for up to 24 h the morphology of preantral follicles in bovine ovaries. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/6128 10.5433/1679-0359.2012v33n1p297 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/6128 |
identifier_str_mv |
10.5433/1679-0359.2012v33n1p297 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/6128/10155 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 33 No. 1 (2012); 297-304 Semina: Ciências Agrárias; v. 33 n. 1 (2012); 297-304 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306063709732864 |