Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil
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Publication Date: | 2019 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Semina. Ciências Agrárias (Online) |
Download full: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34925 |
Summary: | Studies on canine babesiosis in northeastern Brazil are scarce, although the weather conditions in this region are favorable for the development of the tick vector. This study determined the prevalence of Babesia vogeli in dogs sampled in Teresina, state of Piauí, northeast Brazil, using direct and indirect diagnostic methods and performed a phylogenetic analysis of 18S rRNA sequences. A total of 315 dogs were screened during routine care regardless of clinical suspicion. Blood was collected by jugular venipuncture to perform indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) and for parasite screening in peripheral blood smears. Positivity was 2.2% (7/315) by microscopy, 4.8% (15/315) by PCR, and 48.6% (153/315) by IFA. PCR amplified a 602-bp fragment of the piroplasmid 18S rRNA gene, and sequence alignment and analysis revealed 99% homology with B. vogeli isolates from other regions of Brazil and other countries. In addition, there was high variability among sequences from other northeast states of Brazil. This study is the first to perform the molecular analysis of B. vogeli in Piauí. The results demonstrate that canine babesiosis is endemic in dogs sampled in Teresina and that PCR may be the method of choice to perform parasite screening in this region. |
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Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, BrazilMolecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, BrazilMolecular analysisBabesiosisBlood parasitesPCRIFA.Análise molecularBabesioseHemoparasitasPCRRIFI.Studies on canine babesiosis in northeastern Brazil are scarce, although the weather conditions in this region are favorable for the development of the tick vector. This study determined the prevalence of Babesia vogeli in dogs sampled in Teresina, state of Piauí, northeast Brazil, using direct and indirect diagnostic methods and performed a phylogenetic analysis of 18S rRNA sequences. A total of 315 dogs were screened during routine care regardless of clinical suspicion. Blood was collected by jugular venipuncture to perform indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) and for parasite screening in peripheral blood smears. Positivity was 2.2% (7/315) by microscopy, 4.8% (15/315) by PCR, and 48.6% (153/315) by IFA. PCR amplified a 602-bp fragment of the piroplasmid 18S rRNA gene, and sequence alignment and analysis revealed 99% homology with B. vogeli isolates from other regions of Brazil and other countries. In addition, there was high variability among sequences from other northeast states of Brazil. This study is the first to perform the molecular analysis of B. vogeli in Piauí. The results demonstrate that canine babesiosis is endemic in dogs sampled in Teresina and that PCR may be the method of choice to perform parasite screening in this region.Estudos sobre a babesiose canina são escassos no Nordeste do Brasil, apesar das condições climáticas favoráveis ao desenvolvimento do carrapato vetor. Esta pesquisa objetivou determinar a ocorrência de Babesia vogeli em cães amostrados em Teresina, estado do Piauí, região Meio Norte do Brasil, através de métodos diretos e indiretos de diagnóstico, além de realizar análise filogenética das sequências 18S rRNA de piroplasmídeos obtidas no estudo. Foram avaliados 315 cães atendidos em clínicas veterinárias, sob qualquer suspeita clínica. Desses animais, foi colhido sangue por venopunção jugular para Reação de Imunofluorescência Indireta (RIFI) e Reação em Cadeia pela Polimerase (PCR). Além disso, esfregaços de sangue periférico foram realizados para pesquisa direta do parasita. A positividade dos animais foi de 2,2% (7/315) ao esfregaço sanguíneo, 4,8% (15/315) à PCR e 48,6% (153/315) à RIFI. O sequenciamento de amostras positivas à PCR resultou em um fragmento de 602 pb do gene 18S rRNA de piroplasmídeos, cujo alinhamento e análise da sequência revelaram 99% de homologia com isolados de B. vogeli de outras regiões do Brasil, além de outros países. É interessante ressaltar que, comparando isolados em diferentes estados do Nordeste, a homologia pode ser bastante variável. Esses são os primeiros resultados sobre a análise molecular de B. vogeli no Estado do Piauí. Além disso, este estudo demonstra que a babesiose canina é endêmica em cães de Teresina, Nordeste do Brasil, e que a PCR pode ser o método de escolha para diagnóstico da doença nessas áreas.UEL2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionEstudo de ocorrênciaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3492510.5433/1679-0359.2019v40n6Supl2p3035Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3035-3044Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3035-30441679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34925/26128Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBraga, Juliana Fortes VilarinhoSouza, Francisco de Assis LeiteSilva, Lucilene dos SantosFonseca, Luciano Santos daPinho, Flaviane Alves deFotoran, Wesley LuzettiRamos-Sanchez, Eduardo MiltonCosta-Júnior, Lívio MartinsRibeiro, Múcio Flávio BarbosaSilva, Silvana Maria Medeiros de Sousa2022-10-10T16:03:15Zoai:ojs.pkp.sfu.ca:article/34925Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:03:15Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
title |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
spellingShingle |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil Braga, Juliana Fortes Vilarinho Molecular analysis Babesiosis Blood parasites PCR IFA. Análise molecular Babesiose Hemoparasitas PCR RIFI. |
title_short |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
title_full |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
title_fullStr |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
title_full_unstemmed |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
title_sort |
Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil |
author |
Braga, Juliana Fortes Vilarinho |
author_facet |
Braga, Juliana Fortes Vilarinho Souza, Francisco de Assis Leite Silva, Lucilene dos Santos Fonseca, Luciano Santos da Pinho, Flaviane Alves de Fotoran, Wesley Luzetti Ramos-Sanchez, Eduardo Milton Costa-Júnior, Lívio Martins Ribeiro, Múcio Flávio Barbosa Silva, Silvana Maria Medeiros de Sousa |
author_role |
author |
author2 |
Souza, Francisco de Assis Leite Silva, Lucilene dos Santos Fonseca, Luciano Santos da Pinho, Flaviane Alves de Fotoran, Wesley Luzetti Ramos-Sanchez, Eduardo Milton Costa-Júnior, Lívio Martins Ribeiro, Múcio Flávio Barbosa Silva, Silvana Maria Medeiros de Sousa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Braga, Juliana Fortes Vilarinho Souza, Francisco de Assis Leite Silva, Lucilene dos Santos Fonseca, Luciano Santos da Pinho, Flaviane Alves de Fotoran, Wesley Luzetti Ramos-Sanchez, Eduardo Milton Costa-Júnior, Lívio Martins Ribeiro, Múcio Flávio Barbosa Silva, Silvana Maria Medeiros de Sousa |
dc.subject.por.fl_str_mv |
Molecular analysis Babesiosis Blood parasites PCR IFA. Análise molecular Babesiose Hemoparasitas PCR RIFI. |
topic |
Molecular analysis Babesiosis Blood parasites PCR IFA. Análise molecular Babesiose Hemoparasitas PCR RIFI. |
description |
Studies on canine babesiosis in northeastern Brazil are scarce, although the weather conditions in this region are favorable for the development of the tick vector. This study determined the prevalence of Babesia vogeli in dogs sampled in Teresina, state of Piauí, northeast Brazil, using direct and indirect diagnostic methods and performed a phylogenetic analysis of 18S rRNA sequences. A total of 315 dogs were screened during routine care regardless of clinical suspicion. Blood was collected by jugular venipuncture to perform indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) and for parasite screening in peripheral blood smears. Positivity was 2.2% (7/315) by microscopy, 4.8% (15/315) by PCR, and 48.6% (153/315) by IFA. PCR amplified a 602-bp fragment of the piroplasmid 18S rRNA gene, and sequence alignment and analysis revealed 99% homology with B. vogeli isolates from other regions of Brazil and other countries. In addition, there was high variability among sequences from other northeast states of Brazil. This study is the first to perform the molecular analysis of B. vogeli in Piauí. The results demonstrate that canine babesiosis is endemic in dogs sampled in Teresina and that PCR may be the method of choice to perform parasite screening in this region. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Estudo de ocorrência |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34925 10.5433/1679-0359.2019v40n6Supl2p3035 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34925 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6Supl2p3035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34925/26128 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3035-3044 Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3035-3044 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306080915816448 |