Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13600 |
Resumo: | The excessive release of calcium from the sarcoplasmic reticulum during the installation of PSE (Pale, Soft, Exudative) meat, leads to increase of phospholipase A2 (PLA2) activity and consequently causes a higher lipid oxidation. In contrast, glutathione peroxidase (GSH-Px) is a selenium-dependent antioxidant enzyme that prevents the oxidative damage. The aim of this work was to investigate the secreted PLA2 (sPLA2) and GSH-PX activities in PSE poultry meat. Breast meat samples (Pectoralis major m.) (n=24) were obtained from commercial slaughterhouse. Samples were classified as PSE and Control Meat based on pH and L* values, fillets with pH1h30? 6.0 and L24h* ?53.0 as PSE and fillets with pH1h30> 6.0 and L24h*<53.0 with Control. sPLA2 was analyzed using enzymatic assay kit and GSH-Px was measured a coupled assay procedure recording the NADPH (nicotinamide adenine dinucleotide phosphate reduced) oxidation. The sPLA2 average was 0.295 Umg-1 of protein for PSE meat and 0.380 Umg-1 of protein for Control meat, did not differ significantly (p >0,05). The GSH-Px activity was approximately 24,4% higher (p?0,05) for Control meat when compared to PSE meat. The sPLA2 activity of PSE fillets did not changed, however PSE fillets present the enzymatic system of antioxidant defense compromised with lower GSH-PX activity. |
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Semina. Ciências Agrárias (Online) |
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Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meatAtividades de fosfolipase A2 secretada e glutationa peroxidase em filés PSE (pale, soft, exudative) de frangoEnzymespHMeat quality.EnzimaspHQualidade de carne.Ciência e Tecnologia de CarnesThe excessive release of calcium from the sarcoplasmic reticulum during the installation of PSE (Pale, Soft, Exudative) meat, leads to increase of phospholipase A2 (PLA2) activity and consequently causes a higher lipid oxidation. In contrast, glutathione peroxidase (GSH-Px) is a selenium-dependent antioxidant enzyme that prevents the oxidative damage. The aim of this work was to investigate the secreted PLA2 (sPLA2) and GSH-PX activities in PSE poultry meat. Breast meat samples (Pectoralis major m.) (n=24) were obtained from commercial slaughterhouse. Samples were classified as PSE and Control Meat based on pH and L* values, fillets with pH1h30? 6.0 and L24h* ?53.0 as PSE and fillets with pH1h30> 6.0 and L24h*<53.0 with Control. sPLA2 was analyzed using enzymatic assay kit and GSH-Px was measured a coupled assay procedure recording the NADPH (nicotinamide adenine dinucleotide phosphate reduced) oxidation. The sPLA2 average was 0.295 Umg-1 of protein for PSE meat and 0.380 Umg-1 of protein for Control meat, did not differ significantly (p >0,05). The GSH-Px activity was approximately 24,4% higher (p?0,05) for Control meat when compared to PSE meat. The sPLA2 activity of PSE fillets did not changed, however PSE fillets present the enzymatic system of antioxidant defense compromised with lower GSH-PX activity.A liberação excessiva de cálcio do retículo sarcoplasmático durante a instalação de carnes PSE (Pale, Soft, Exudative) leva a um aumento da atividade da fosfolipase A2 (PLA2), promovendo uma maior oxidação lipídica. Por outro lado, a glutationa peroxidase (GSH-Px) é uma enzima antioxidante selênio dependente, prevenindo danos oxidativos. O objetivo deste trabalho foi investigar as atividades da PLA2 secretada (sPLA2) e da GSH-PX em filés PSE de frango. Filés de frango (Pectoralis major m.) (n=24) foram obtidos de um frigorífico comercial. As amostras foram classificadas em PSE e Controle, com base nos valores de pH e L*, filés com pH1h30 ? 6.0 e L24h* ? 53,0 como PSE e filés com pH1h30> 6,0 e L24h* < 53,0 como Controle. A atividade da sPLA2 foi determinada por kit enzimático e a atividade da GSH-Px foi determinada pela oxidação do NADPH (Nicotinamida Adenina Dinucleotídeo Fosfato Reduzido). A atividade média da sPLA2 foi de 0,295 U.mg-1 de proteína para os filés PSE e de 0,380 U.mg-1 de proteína para os filés do Controle, não apresentado diferenças significativas (p >0,05). A atividade da GSH-Px foi aproximadamente 24,4% maior (p?0,05) para os filés Controle quando comparado com filés PSE. A atividade da sPLA2 em filés PSE não foi alterada, entretanto filés PSE apresentam o sistema enzimático de defesa antioxidante comprometido com menor atividade de GSH-Px.UEL2013-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa empíricaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1360010.5433/1679-0359.2012v33n6Supl2p3111Semina: Ciências Agrárias; Vol. 33 No. 6Supl2 (2012); 3111-3116Semina: Ciências Agrárias; v. 33 n. 6Supl2 (2012); 3111-31161679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13600/pdfCopyright (c) 2013 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAlmeida, Gleice Rocha SantosMarchi, Denis FabricioAlmeida, Juliana Nunes deMendonça, Fernanda JessicaShimokomaki, MassamiSoares, Adriana Lourenço2023-01-26T15:38:45Zoai:ojs.pkp.sfu.ca:article/13600Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-26T15:38:45Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat Atividades de fosfolipase A2 secretada e glutationa peroxidase em filés PSE (pale, soft, exudative) de frango |
title |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat |
spellingShingle |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat Almeida, Gleice Rocha Santos Enzymes pH Meat quality. Enzimas pH Qualidade de carne. Ciência e Tecnologia de Carnes |
title_short |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat |
title_full |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat |
title_fullStr |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat |
title_full_unstemmed |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat |
title_sort |
Secreted phospholipase A2 and glutathione peroxidase activities in chicken PSE (pale, soft, exudative) meat |
author |
Almeida, Gleice Rocha Santos |
author_facet |
Almeida, Gleice Rocha Santos Marchi, Denis Fabricio Almeida, Juliana Nunes de Mendonça, Fernanda Jessica Shimokomaki, Massami Soares, Adriana Lourenço |
author_role |
author |
author2 |
Marchi, Denis Fabricio Almeida, Juliana Nunes de Mendonça, Fernanda Jessica Shimokomaki, Massami Soares, Adriana Lourenço |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Gleice Rocha Santos Marchi, Denis Fabricio Almeida, Juliana Nunes de Mendonça, Fernanda Jessica Shimokomaki, Massami Soares, Adriana Lourenço |
dc.subject.por.fl_str_mv |
Enzymes pH Meat quality. Enzimas pH Qualidade de carne. Ciência e Tecnologia de Carnes |
topic |
Enzymes pH Meat quality. Enzimas pH Qualidade de carne. Ciência e Tecnologia de Carnes |
description |
The excessive release of calcium from the sarcoplasmic reticulum during the installation of PSE (Pale, Soft, Exudative) meat, leads to increase of phospholipase A2 (PLA2) activity and consequently causes a higher lipid oxidation. In contrast, glutathione peroxidase (GSH-Px) is a selenium-dependent antioxidant enzyme that prevents the oxidative damage. The aim of this work was to investigate the secreted PLA2 (sPLA2) and GSH-PX activities in PSE poultry meat. Breast meat samples (Pectoralis major m.) (n=24) were obtained from commercial slaughterhouse. Samples were classified as PSE and Control Meat based on pH and L* values, fillets with pH1h30? 6.0 and L24h* ?53.0 as PSE and fillets with pH1h30> 6.0 and L24h*<53.0 with Control. sPLA2 was analyzed using enzymatic assay kit and GSH-Px was measured a coupled assay procedure recording the NADPH (nicotinamide adenine dinucleotide phosphate reduced) oxidation. The sPLA2 average was 0.295 Umg-1 of protein for PSE meat and 0.380 Umg-1 of protein for Control meat, did not differ significantly (p >0,05). The GSH-Px activity was approximately 24,4% higher (p?0,05) for Control meat when compared to PSE meat. The sPLA2 activity of PSE fillets did not changed, however PSE fillets present the enzymatic system of antioxidant defense compromised with lower GSH-PX activity. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa empírica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13600 10.5433/1679-0359.2012v33n6Supl2p3111 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13600 |
identifier_str_mv |
10.5433/1679-0359.2012v33n6Supl2p3111 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13600/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 33 No. 6Supl2 (2012); 3111-3116 Semina: Ciências Agrárias; v. 33 n. 6Supl2 (2012); 3111-3116 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306068494385152 |