Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753 |
Resumo: | The objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by the disc diffusion test using different concentrations of the oils: 160, 80, 40, 20 and 10 ?l ml -1. The multi-resistance of strains relative to that of conventional antimicrobials was also evaluated by the disk diffusion technique, using Multiple Antibiotic Resistance (MAR) index. The oils were characterized based on the results of chromatographic analyses, of which, analysis of lemongrass has been previously published (AZEVEDO et al., 2016). Lemongrass and clove essential oils presented citral and eugenol as the major compound, respectively. The concentrations of the essential oils had a significant effect on the extent of the growth inhibition halo and the coefficient of determination (r²) was higher than 0.80. Clove essential oil generated the largest zone of inhibition when tested against Escherichia coli and S. aureus from poultry feces and Salmonella sp. from the feces of swine, while lemongrass essential oil presented better results against S. aureus isolated from swine feces and Salmonella sp. from bovine feces. S. aureus and Salmonella sp. were multi-resistant to the antimicrobials tested. It is concluded that the essential oils tested have antimicrobial activity against bacteria isolated from bovine, swine, and poultry feces and that this activity is proportional to the concentration of oils and the microorganisms studied. |
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Semina. Ciências Agrárias (Online) |
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Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry fecesAtividade antimicrobiana de óleos essenciais extraídos de cravo-da-índia e capim-limão contra bactérias patogênicas isoladas de fezes de bovinos, suínos e avesAntimicrobialCloveCymbopogon flexuosusLemongrassSyzygium aromaticum.AntimicrobianoCravo-da-índiaCymbopogon flexuosusCapim-limãoSyzygium aromaticum.The objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by the disc diffusion test using different concentrations of the oils: 160, 80, 40, 20 and 10 ?l ml -1. The multi-resistance of strains relative to that of conventional antimicrobials was also evaluated by the disk diffusion technique, using Multiple Antibiotic Resistance (MAR) index. The oils were characterized based on the results of chromatographic analyses, of which, analysis of lemongrass has been previously published (AZEVEDO et al., 2016). Lemongrass and clove essential oils presented citral and eugenol as the major compound, respectively. The concentrations of the essential oils had a significant effect on the extent of the growth inhibition halo and the coefficient of determination (r²) was higher than 0.80. Clove essential oil generated the largest zone of inhibition when tested against Escherichia coli and S. aureus from poultry feces and Salmonella sp. from the feces of swine, while lemongrass essential oil presented better results against S. aureus isolated from swine feces and Salmonella sp. from bovine feces. S. aureus and Salmonella sp. were multi-resistant to the antimicrobials tested. It is concluded that the essential oils tested have antimicrobial activity against bacteria isolated from bovine, swine, and poultry feces and that this activity is proportional to the concentration of oils and the microorganisms studied.Objetivou-se verificar a atividade antibacteriana dos óleos de capim-limão e cravo-da-índia frente à Escherichia coli isolada de fezes de aves, Staphylococcus aureus isolado de fezes de suínos e aves e Salmonella sp. isolado de fezes de suínos e bovinos. A atividade antimicrobiana foi avaliada pelo teste de difusão em disco utilizando diferentes concentrações dos óleos: 160, 80, 40, 20 e 10 ?l ml -1. A multirresistência de cepas em relação à antimicrobiana convencional também foi avaliada pela técnica de disco-difusão, utilizando-se o índice de resistência múltipla a antibióticos (MAR). Os óleos foram caracterizados com base nos resultados de análises cromatográficas, das quais, a análise de capim-limão já foi publicada anteriormente (AZEVEDO et al., 2016). Os óleos essenciais de erva-cidreira e cravo apresentaram citral e eugenol como o principal composto, respectivamente. As concentrações dos óleos essenciais tiveram um efeito significativo no comprimento do halo de inibição do crescimento e o coeficiente de determinação (r²) foi superior a 0,80. O óleo essencial de cravo-da-índia gerou a maior zona de inibição quando testado frente á Escherichia coli e S. aureus a partir de fezes de aves e Salmonella sp. das fezes de suínos, enquanto o óleo essencial de capim-limão apresentou melhores resultados frente á S. aureus isolado de fezes de suínos e Salmonella sp. de fezes de bovinos. S. aureus e Salmonella sp. foram multirresistentes aos antimicrobianos testados. Conclui-se que os óleos essenciais testados possuem atividade antimicrobiana contra bactérias isoladas de fezes de bovinos, suínos e aves e que essa atividade é proporcional à concentração de óleos e microrganismos estudados.UEL2019-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3375310.5433/1679-0359.2019v40n5p1937Semina: Ciências Agrárias; Vol. 40 No. 5 (2019); 1937-1950Semina: Ciências Agrárias; v. 40 n. 5 (2019); 1937-19501679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753/25650Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessOliveira, Stephanie Pedrosa deCunha, Gabriel Santos PersiquiniPrates, João Paulo BicalhoFonseca, Francine Souza AlvesSouza, Keicy Sandy Silvestre deAzevedo, Alcinei MísticoXavier, Alessandra Rejane Ericsson de OliveiraSantos, Eliane Macedo SobrinhoSantos, Hércules OtacílioAlmeida, Anna Christina de2022-10-19T11:57:22Zoai:ojs.pkp.sfu.ca:article/33753Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T11:57:22Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces Atividade antimicrobiana de óleos essenciais extraídos de cravo-da-índia e capim-limão contra bactérias patogênicas isoladas de fezes de bovinos, suínos e aves |
title |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
spellingShingle |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces Oliveira, Stephanie Pedrosa de Antimicrobial Clove Cymbopogon flexuosus Lemongrass Syzygium aromaticum. Antimicrobiano Cravo-da-índia Cymbopogon flexuosus Capim-limão Syzygium aromaticum. |
title_short |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
title_full |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
title_fullStr |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
title_full_unstemmed |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
title_sort |
Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
author |
Oliveira, Stephanie Pedrosa de |
author_facet |
Oliveira, Stephanie Pedrosa de Cunha, Gabriel Santos Persiquini Prates, João Paulo Bicalho Fonseca, Francine Souza Alves Souza, Keicy Sandy Silvestre de Azevedo, Alcinei Místico Xavier, Alessandra Rejane Ericsson de Oliveira Santos, Eliane Macedo Sobrinho Santos, Hércules Otacílio Almeida, Anna Christina de |
author_role |
author |
author2 |
Cunha, Gabriel Santos Persiquini Prates, João Paulo Bicalho Fonseca, Francine Souza Alves Souza, Keicy Sandy Silvestre de Azevedo, Alcinei Místico Xavier, Alessandra Rejane Ericsson de Oliveira Santos, Eliane Macedo Sobrinho Santos, Hércules Otacílio Almeida, Anna Christina de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Stephanie Pedrosa de Cunha, Gabriel Santos Persiquini Prates, João Paulo Bicalho Fonseca, Francine Souza Alves Souza, Keicy Sandy Silvestre de Azevedo, Alcinei Místico Xavier, Alessandra Rejane Ericsson de Oliveira Santos, Eliane Macedo Sobrinho Santos, Hércules Otacílio Almeida, Anna Christina de |
dc.subject.por.fl_str_mv |
Antimicrobial Clove Cymbopogon flexuosus Lemongrass Syzygium aromaticum. Antimicrobiano Cravo-da-índia Cymbopogon flexuosus Capim-limão Syzygium aromaticum. |
topic |
Antimicrobial Clove Cymbopogon flexuosus Lemongrass Syzygium aromaticum. Antimicrobiano Cravo-da-índia Cymbopogon flexuosus Capim-limão Syzygium aromaticum. |
description |
The objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by the disc diffusion test using different concentrations of the oils: 160, 80, 40, 20 and 10 ?l ml -1. The multi-resistance of strains relative to that of conventional antimicrobials was also evaluated by the disk diffusion technique, using Multiple Antibiotic Resistance (MAR) index. The oils were characterized based on the results of chromatographic analyses, of which, analysis of lemongrass has been previously published (AZEVEDO et al., 2016). Lemongrass and clove essential oils presented citral and eugenol as the major compound, respectively. The concentrations of the essential oils had a significant effect on the extent of the growth inhibition halo and the coefficient of determination (r²) was higher than 0.80. Clove essential oil generated the largest zone of inhibition when tested against Escherichia coli and S. aureus from poultry feces and Salmonella sp. from the feces of swine, while lemongrass essential oil presented better results against S. aureus isolated from swine feces and Salmonella sp. from bovine feces. S. aureus and Salmonella sp. were multi-resistant to the antimicrobials tested. It is concluded that the essential oils tested have antimicrobial activity against bacteria isolated from bovine, swine, and poultry feces and that this activity is proportional to the concentration of oils and the microorganisms studied. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753 10.5433/1679-0359.2019v40n5p1937 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753 |
identifier_str_mv |
10.5433/1679-0359.2019v40n5p1937 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753/25650 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 5 (2019); 1937-1950 Semina: Ciências Agrárias; v. 40 n. 5 (2019); 1937-1950 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306080279330816 |