Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract

Detalhes bibliográficos
Autor(a) principal: Rosset, Michele
Data de Publicação: 2005
Outros Autores: Zamarion, Vitor de Moraes, Faccione, Milton, Faria, Terezinha de Jesus, Pinto, Jurandir Pereira, Barbosa, Aneli de Melo, Souza, José Roberto Pinto de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2340
Resumo: Previous studies showed that ethanolic extract of Ocimum gratissimum inhibited growth of Penicillium chrysogenum, Rhizoctonia sp, Aspergillus niger and Alternaria sp, isolated from tomato and carrot. The aim of this study was to identify the chemical constituents of Ocimum gratissim. Aerial parts of the plant were treated with hexane-dichloromethane-ethanol mixture (1:3:1) at room temperature. The obtained extract was chromatographed on silica gel column eluted with hexane, dichloromethane and ethanol. Fractions eluted with dichloromethane were rechromatographed on a column of silica gel to yield alyphatic hydrocarbon mixture (C31, C33, C34 e C35), eugenol, caryophyllene oxide and a mixture of stigmasterol and sitosterol. The structures of the compounds were deduced by spectral data analysis and by comparison with literature values. Recent studies showed that eugenol is probably one of the responsibles for antifungal activity of O. gratissimum.
id UEL-11_e3ef4a4a46534773d10d78a451a33c0f
oai_identifier_str oai:ojs.pkp.sfu.ca:article/2340
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extractEstudo químico da fração diclorometânica do extrato de Ocimum gratissimum L.Ocimum gratissimumAntifungal activityEugenolPhytopathogenic fungi.Ocimum gratissimumAtividade antifúngicaEugenolFungos fitopatogênicos.Previous studies showed that ethanolic extract of Ocimum gratissimum inhibited growth of Penicillium chrysogenum, Rhizoctonia sp, Aspergillus niger and Alternaria sp, isolated from tomato and carrot. The aim of this study was to identify the chemical constituents of Ocimum gratissim. Aerial parts of the plant were treated with hexane-dichloromethane-ethanol mixture (1:3:1) at room temperature. The obtained extract was chromatographed on silica gel column eluted with hexane, dichloromethane and ethanol. Fractions eluted with dichloromethane were rechromatographed on a column of silica gel to yield alyphatic hydrocarbon mixture (C31, C33, C34 e C35), eugenol, caryophyllene oxide and a mixture of stigmasterol and sitosterol. The structures of the compounds were deduced by spectral data analysis and by comparison with literature values. Recent studies showed that eugenol is probably one of the responsibles for antifungal activity of O. gratissimum.Ensaios anteriores de atividade antifúngica do extrato etanólico de Ocimum gratissimum, utilizando a técnica de difusão em meio sólido, evidenciaram a inibição do crescimento de Penicillium chrysogenum, Rhizoctonia sp, Aspergillus niger e de duas espécies de Alternaria sp isoladas de tomate e de cenoura. O objetivo deste trabalho foi identificar os possíveis constituintes químicos responsáveis pela atividade antifúngica da planta, por meio de estudo químico do extrato hexano-diclorometano-etanol (1:3:1) das partes aéreas de O. gratissimum. O extrato foi cromatografado em coluna de sílica gel, eluída com hexano, diclorometano e etanol. A purificação em coluna cromatográfica de sílica gel das frações eluídas com diclorometano (5,9g) resultou na obtenção de quatro constituintes: uma mistura de hidrocarbonetos saturados de cadeia longa (C31, C33, C34 e C35), eugenol, óxido de cariofileno e uma mistura de estigmasterolsitosterol. Os constituintes foram identificados através das análises por cromatografia gasosa acoplada à espectrometria de massas e das espectrometrias no infravermelho, de ressonância magnética nuclear de 1H e de 13C e comparação com dados espectrais da literatura. Entre os constituintes isolados, concluiuse que o eugenol é um dos prováveis responsáveis pela atividade antifúngica de O. gratissimum, pois registros da literatura comprovaram a atividade deste constituinte contra várias espécies de fungos.UEL2005-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/234010.5433/1679-0359.2005v26n4p515Semina: Ciências Agrárias; Vol. 26 No. 4 (2005); 515-520Semina: Ciências Agrárias; v. 26 n. 4 (2005); 515-5201679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2340/2013Rosset, MicheleZamarion, Vitor de MoraesFaccione, MiltonFaria, Terezinha de JesusPinto, Jurandir PereiraBarbosa, Aneli de MeloSouza, José Roberto Pinto deinfo:eu-repo/semantics/openAccess2009-10-08T17:47:17Zoai:ojs.pkp.sfu.ca:article/2340Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-10-08T17:47:17Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
Estudo químico da fração diclorometânica do extrato de Ocimum gratissimum L.
title Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
spellingShingle Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
Rosset, Michele
Ocimum gratissimum
Antifungal activity
Eugenol
Phytopathogenic fungi.
Ocimum gratissimum
Atividade antifúngica
Eugenol
Fungos fitopatogênicos.
title_short Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
title_full Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
title_fullStr Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
title_full_unstemmed Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
title_sort Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract
author Rosset, Michele
author_facet Rosset, Michele
Zamarion, Vitor de Moraes
Faccione, Milton
Faria, Terezinha de Jesus
Pinto, Jurandir Pereira
Barbosa, Aneli de Melo
Souza, José Roberto Pinto de
author_role author
author2 Zamarion, Vitor de Moraes
Faccione, Milton
Faria, Terezinha de Jesus
Pinto, Jurandir Pereira
Barbosa, Aneli de Melo
Souza, José Roberto Pinto de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rosset, Michele
Zamarion, Vitor de Moraes
Faccione, Milton
Faria, Terezinha de Jesus
Pinto, Jurandir Pereira
Barbosa, Aneli de Melo
Souza, José Roberto Pinto de
dc.subject.por.fl_str_mv Ocimum gratissimum
Antifungal activity
Eugenol
Phytopathogenic fungi.
Ocimum gratissimum
Atividade antifúngica
Eugenol
Fungos fitopatogênicos.
topic Ocimum gratissimum
Antifungal activity
Eugenol
Phytopathogenic fungi.
Ocimum gratissimum
Atividade antifúngica
Eugenol
Fungos fitopatogênicos.
description Previous studies showed that ethanolic extract of Ocimum gratissimum inhibited growth of Penicillium chrysogenum, Rhizoctonia sp, Aspergillus niger and Alternaria sp, isolated from tomato and carrot. The aim of this study was to identify the chemical constituents of Ocimum gratissim. Aerial parts of the plant were treated with hexane-dichloromethane-ethanol mixture (1:3:1) at room temperature. The obtained extract was chromatographed on silica gel column eluted with hexane, dichloromethane and ethanol. Fractions eluted with dichloromethane were rechromatographed on a column of silica gel to yield alyphatic hydrocarbon mixture (C31, C33, C34 e C35), eugenol, caryophyllene oxide and a mixture of stigmasterol and sitosterol. The structures of the compounds were deduced by spectral data analysis and by comparison with literature values. Recent studies showed that eugenol is probably one of the responsibles for antifungal activity of O. gratissimum.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2340
10.5433/1679-0359.2005v26n4p515
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2340
identifier_str_mv 10.5433/1679-0359.2005v26n4p515
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2340/2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 26 No. 4 (2005); 515-520
Semina: Ciências Agrárias; v. 26 n. 4 (2005); 515-520
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306057984507904

Registros relacionados