Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40707 |
Resumo: | Feline coronavirus (FCoV) is an important virus that can be differentiated into two serotypes: feline enteric coronavirus (FECoV) and feline infectious peritonitis (FIP) virus (FIPV). Researchers have suggested that a mutation of FECoV to FIPV leads to the emergence of FIP, a disease with worldwide distribution and a high mortality rate. Furthermore, in December 2019, a human infectious disease, coronavirus disease-2019 (COVID-19), which is also caused by a coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) emerged, and clarity regarding its relationship with FCoV remains lacking. Studies have shown that cats are susceptible to infection with this novel coronavirus (i.e., SARS-CoV-2). The aim of the present study was to detect and semi-quantify the presence of feline antibodies to FIPV in cats examined at the Veterinary Hospital of Santa Cruz State University, microregion of Ilhéus and Itabuna, Bahia, Brazil, between January and April 2018. Blood samples were collected from 68 domestic cats to perform complete blood count (CBC) and biochemical tests, and an indirect fluorescent antibody test (IFAT) was used to detect FCoV infection. Of the 68 samples evaluated, seropositivity was observed in 4.4% (3/68) at titers of 1:20; only one sample remained seropositive at titers of 1:40 and 1:80. Two positive animals exhibited CBC and biochemical values within the normal range, while the other positive animals exhibited a mild decrease in platelet count (173,000 uL-1), mild lymphocytosis (7395 uL-1), and mildly increased alkaline phosphatase level (134 uL-1). Twelve months after the tests, none of the positive animals exhibited clinical signs consistent with FIP. Although the IFAT can facilitate diagnosis of FPIV, it cannot be used to differentiate antibodies for the FECoV and FIPV serotypes. Results of the present study demonstrated that FCoV was present in the population studied, and is an important risk factor for the development of FIP. In addition, the new COVID-19 pandemic highlights the importance of studies investigating FCoV because it was not possible to rule out, until now, the possibility of FCoV mutations in infected cats if it encounters SARS-CoV-2. |
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Semina. Ciências Agrárias (Online) |
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Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, BrazilDetecção e semiquantificação de anticorpos para o Vírus da Peritonite Infecciosa Felina em gatos da microregião Ilhéus-Itabuna, Bahia, BrasilEnteric CoronavirusFelinesInfectious peritonitisSerology.Coronavírus entéricoFelinosPeritonite infecciosaSorologia.Feline coronavirus (FCoV) is an important virus that can be differentiated into two serotypes: feline enteric coronavirus (FECoV) and feline infectious peritonitis (FIP) virus (FIPV). Researchers have suggested that a mutation of FECoV to FIPV leads to the emergence of FIP, a disease with worldwide distribution and a high mortality rate. Furthermore, in December 2019, a human infectious disease, coronavirus disease-2019 (COVID-19), which is also caused by a coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) emerged, and clarity regarding its relationship with FCoV remains lacking. Studies have shown that cats are susceptible to infection with this novel coronavirus (i.e., SARS-CoV-2). The aim of the present study was to detect and semi-quantify the presence of feline antibodies to FIPV in cats examined at the Veterinary Hospital of Santa Cruz State University, microregion of Ilhéus and Itabuna, Bahia, Brazil, between January and April 2018. Blood samples were collected from 68 domestic cats to perform complete blood count (CBC) and biochemical tests, and an indirect fluorescent antibody test (IFAT) was used to detect FCoV infection. Of the 68 samples evaluated, seropositivity was observed in 4.4% (3/68) at titers of 1:20; only one sample remained seropositive at titers of 1:40 and 1:80. Two positive animals exhibited CBC and biochemical values within the normal range, while the other positive animals exhibited a mild decrease in platelet count (173,000 uL-1), mild lymphocytosis (7395 uL-1), and mildly increased alkaline phosphatase level (134 uL-1). Twelve months after the tests, none of the positive animals exhibited clinical signs consistent with FIP. Although the IFAT can facilitate diagnosis of FPIV, it cannot be used to differentiate antibodies for the FECoV and FIPV serotypes. Results of the present study demonstrated that FCoV was present in the population studied, and is an important risk factor for the development of FIP. In addition, the new COVID-19 pandemic highlights the importance of studies investigating FCoV because it was not possible to rule out, until now, the possibility of FCoV mutations in infected cats if it encounters SARS-CoV-2.O coronavírus felino (FCoV) é um importante vírus que pode ser diferenciado em dois sorotipos: coronavírus entérico felino (FECoV) e vírus da peritonite infecciosa felina (VPIF). Pesquisas sugerem que uma mutação do FECoV para o FIPV leva ao surgimento da peritonite infecciosa felina (PIF). Esta doença possui distribuição mundial e alta taxa de mortalidade. O objetivo desse estudo foi detectar e semiquantificar presença de anticorpos para o FECoV em gatos examinados no Hospital Veterinário da Universidade Estadual de Santa Cruz, microrregião de Ilhéus e Itabuna, na Bahia, de janeiro a abril de 2018. Foram colhidas amostras de sangue de 68 felinos domésticos para realização de hemograma e testes bioquímicos. Para detecção de FCoV foi utilizada a imunofluorescência indireta (IFI). Das 68 amostras avaliadas, observou-se soropositividade em 4,4% (3/68) das amostras com títulos de 1:20, e para os títulos 1:40 e 1:80 apenas uma amostra continuou soropositiva. Dois animais positivos apresentaram hemograma e dosagens bioquímicas dentro da normalidade, enquanto o outro animal positivo apresentou discreta diminuição na contagem de plaquetas (173 mil uL-1), discreta linfocitose (7395 uL-1) e fosfatase alcalina discretamente aumentada (134 uL-1). Passados doze meses das realizações dos exames, nenhum dos animais positivos apresentou sinais clínicos compatíveis com PIF. A ocorrência encontrada nesse estudo demonstra que há presença do FCoV na população estudada, tornando-se um importante fator de risco para a infecção.UEL2021-02-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4070710.5433/1679-0359.2021v42n2p747Semina: Ciências Agrárias; Vol. 42 No. 2 (2021); 747-756Semina: Ciências Agrárias; v. 42 n. 2 (2021); 747-7561679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40707/29035Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessVeloso, Jéssica FontesSauer, LeonardoSilva, Lohana Mehnati Costa ePellizzoni, Samantha GusmãoGuedes, Paula Elisa BrandãoCarlos, Renata Santiago Alberto2022-10-04T14:10:36Zoai:ojs.pkp.sfu.ca:article/40707Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T14:10:36Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil Detecção e semiquantificação de anticorpos para o Vírus da Peritonite Infecciosa Felina em gatos da microregião Ilhéus-Itabuna, Bahia, Brasil |
title |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil |
spellingShingle |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil Veloso, Jéssica Fontes Enteric Coronavirus Felines Infectious peritonitis Serology. Coronavírus entérico Felinos Peritonite infecciosa Sorologia. |
title_short |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil |
title_full |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil |
title_fullStr |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil |
title_full_unstemmed |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil |
title_sort |
Detection and semi-quantification of antibody to feline coronavirus in cats from the microregion of Ilhéus-Itabuna, Bahia, Brazil |
author |
Veloso, Jéssica Fontes |
author_facet |
Veloso, Jéssica Fontes Sauer, Leonardo Silva, Lohana Mehnati Costa e Pellizzoni, Samantha Gusmão Guedes, Paula Elisa Brandão Carlos, Renata Santiago Alberto |
author_role |
author |
author2 |
Sauer, Leonardo Silva, Lohana Mehnati Costa e Pellizzoni, Samantha Gusmão Guedes, Paula Elisa Brandão Carlos, Renata Santiago Alberto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Veloso, Jéssica Fontes Sauer, Leonardo Silva, Lohana Mehnati Costa e Pellizzoni, Samantha Gusmão Guedes, Paula Elisa Brandão Carlos, Renata Santiago Alberto |
dc.subject.por.fl_str_mv |
Enteric Coronavirus Felines Infectious peritonitis Serology. Coronavírus entérico Felinos Peritonite infecciosa Sorologia. |
topic |
Enteric Coronavirus Felines Infectious peritonitis Serology. Coronavírus entérico Felinos Peritonite infecciosa Sorologia. |
description |
Feline coronavirus (FCoV) is an important virus that can be differentiated into two serotypes: feline enteric coronavirus (FECoV) and feline infectious peritonitis (FIP) virus (FIPV). Researchers have suggested that a mutation of FECoV to FIPV leads to the emergence of FIP, a disease with worldwide distribution and a high mortality rate. Furthermore, in December 2019, a human infectious disease, coronavirus disease-2019 (COVID-19), which is also caused by a coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) emerged, and clarity regarding its relationship with FCoV remains lacking. Studies have shown that cats are susceptible to infection with this novel coronavirus (i.e., SARS-CoV-2). The aim of the present study was to detect and semi-quantify the presence of feline antibodies to FIPV in cats examined at the Veterinary Hospital of Santa Cruz State University, microregion of Ilhéus and Itabuna, Bahia, Brazil, between January and April 2018. Blood samples were collected from 68 domestic cats to perform complete blood count (CBC) and biochemical tests, and an indirect fluorescent antibody test (IFAT) was used to detect FCoV infection. Of the 68 samples evaluated, seropositivity was observed in 4.4% (3/68) at titers of 1:20; only one sample remained seropositive at titers of 1:40 and 1:80. Two positive animals exhibited CBC and biochemical values within the normal range, while the other positive animals exhibited a mild decrease in platelet count (173,000 uL-1), mild lymphocytosis (7395 uL-1), and mildly increased alkaline phosphatase level (134 uL-1). Twelve months after the tests, none of the positive animals exhibited clinical signs consistent with FIP. Although the IFAT can facilitate diagnosis of FPIV, it cannot be used to differentiate antibodies for the FECoV and FIPV serotypes. Results of the present study demonstrated that FCoV was present in the population studied, and is an important risk factor for the development of FIP. In addition, the new COVID-19 pandemic highlights the importance of studies investigating FCoV because it was not possible to rule out, until now, the possibility of FCoV mutations in infected cats if it encounters SARS-CoV-2. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40707 10.5433/1679-0359.2021v42n2p747 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40707 |
identifier_str_mv |
10.5433/1679-0359.2021v42n2p747 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40707/29035 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 2 (2021); 747-756 Semina: Ciências Agrárias; v. 42 n. 2 (2021); 747-756 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306083408281600 |