Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20921 |
Resumo: | In order to investigate the effect of different doses of essential oil of Moringa oleifera (EOM) on physiological potential and the expression of isoenzymes in bean seeds, an experiment was conducted with two cultivars (Colibri and Campos Gerais) and seven treatments (bactericidal, fungicide and the doses 0, 0.1, 0.2, 0.4 and 0.8% EOM). Germination, first count of germination, germination speed index (GSI), emergence and emergence rate index (ERI) were evaluated in addition to the enzymes esterase (EST), alcohol dehydrogenase (ADH), superoxide dismutase (SOD) and isocitrate lyase (ICL) by electrophoresis. In an experiment conducted in the laboratory, the dose containing 0.4% EOM promoted germination and higher averages for first germination in seeds and GSI in the Colibri cultivar. However, the highest dose used (0.8%) was responsible for the worst physiological performance by the cultivar. For Campos Gerais, increasing doses of essential oil stimulated germination, first count of germination and GSI. In the field, higher doses of essential oil gave higher germination percentages for the Colibri cultivar, not influencing the seeds of Campos Gerais. The ERI was not altered by the treatments with OEM and changes in the molecular forms of the isoenzymes were observed, with low expression values of bands for EST, ICL and ADH-related vigor, the germination of both cultivars, as well as increased expression of SOD, which is associated with reductions in germination. |
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Semina. Ciências Agrárias (Online) |
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Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera LamDesempenho fisiológico e padrão eletroforético de isoenzimas em sementes de Phaseolus vulgaris Lam. tratadas com óleo essencial de Moringa oleifera LamBeanGerminationMoringaVigor.FeijãoGerminaçãoMoringaVigor.In order to investigate the effect of different doses of essential oil of Moringa oleifera (EOM) on physiological potential and the expression of isoenzymes in bean seeds, an experiment was conducted with two cultivars (Colibri and Campos Gerais) and seven treatments (bactericidal, fungicide and the doses 0, 0.1, 0.2, 0.4 and 0.8% EOM). Germination, first count of germination, germination speed index (GSI), emergence and emergence rate index (ERI) were evaluated in addition to the enzymes esterase (EST), alcohol dehydrogenase (ADH), superoxide dismutase (SOD) and isocitrate lyase (ICL) by electrophoresis. In an experiment conducted in the laboratory, the dose containing 0.4% EOM promoted germination and higher averages for first germination in seeds and GSI in the Colibri cultivar. However, the highest dose used (0.8%) was responsible for the worst physiological performance by the cultivar. For Campos Gerais, increasing doses of essential oil stimulated germination, first count of germination and GSI. In the field, higher doses of essential oil gave higher germination percentages for the Colibri cultivar, not influencing the seeds of Campos Gerais. The ERI was not altered by the treatments with OEM and changes in the molecular forms of the isoenzymes were observed, with low expression values of bands for EST, ICL and ADH-related vigor, the germination of both cultivars, as well as increased expression of SOD, which is associated with reductions in germination.A fim de investigar o efeito de diferentes doses de óleo essencial de Moringa oleifera (OEM) no potencial fisiológico e expressão de isoenzimas em sementes de feijão, conduziu-se um experimento com duas cultivares (Campos Gerais e Colibri) e sete tratamentos (bactericida, fungicida e as doses 0, 0.1, 0.2, 0.4 e 0.8% de óleo essencial). Foram avaliados a germinação, primeira contagem de germinação, índice de velocidade de germinação (IVG), emergência e índice de velocidade de emergência (IVE), além das enzimas esterase (EST), álcool desidrogenase (ADH), superóxido dismutase (SOD) e isocitrato liase (ICL) por meio de eletroforese. Em experimento conduzido em laboratório, a dose contendo 0.4% de OEM promoveu incremento na germinação e maiores médias para primeira contagem de germinação e IVG nas sementes da cultivar Colibri. No entanto, a maior dose utilizada (0.8%) foi responsável pelo pior desempenho fisiológico para a cultivar. Para Campos Gerais, as doses crescentes de óleo essencial estimularam a germinação, a primeira contagem de germinação e IVG. À campo, as doses de óleo essencial conferiram maior porcentagem de emergência para a cultivar Colibri, não influenciando as sementes da cultivar Campos Gerais. O IVE não sofreu alteração e modificações nas formas moleculares das isoenzimas foram observadas, estando os baixos valores de expressão de bandas para EST, ICL e ADH, relacionadas ao maior vigor e germinação das sementes de ambas as cultivares, bem como, maior expressão de SOD, associada à redução na germinação.UEL2016-04-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2092110.5433/1679-0359.2016v37n2p625Semina: Ciências Agrárias; Vol. 37 No. 2 (2016); 625-638Semina: Ciências Agrárias; v. 37 n. 2 (2016); 625-6381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20921/18583http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAgustini, Márcia Antonia BartolomeuMalavasi, Marlene de MatosStangarlin, José RenatoSchuelter, Adilson RickenMalavasi, Ubirajara ControSantos, Heloisa Oliveira dosPereira, Rucyan Walace2022-12-01T16:04:17Zoai:ojs.pkp.sfu.ca:article/20921Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-01T16:04:17Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam Desempenho fisiológico e padrão eletroforético de isoenzimas em sementes de Phaseolus vulgaris Lam. tratadas com óleo essencial de Moringa oleifera Lam |
title |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam |
spellingShingle |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam Agustini, Márcia Antonia Bartolomeu Bean Germination Moringa Vigor. Feijão Germinação Moringa Vigor. |
title_short |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam |
title_full |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam |
title_fullStr |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam |
title_full_unstemmed |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam |
title_sort |
Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam |
author |
Agustini, Márcia Antonia Bartolomeu |
author_facet |
Agustini, Márcia Antonia Bartolomeu Malavasi, Marlene de Matos Stangarlin, José Renato Schuelter, Adilson Ricken Malavasi, Ubirajara Contro Santos, Heloisa Oliveira dos Pereira, Rucyan Walace |
author_role |
author |
author2 |
Malavasi, Marlene de Matos Stangarlin, José Renato Schuelter, Adilson Ricken Malavasi, Ubirajara Contro Santos, Heloisa Oliveira dos Pereira, Rucyan Walace |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Agustini, Márcia Antonia Bartolomeu Malavasi, Marlene de Matos Stangarlin, José Renato Schuelter, Adilson Ricken Malavasi, Ubirajara Contro Santos, Heloisa Oliveira dos Pereira, Rucyan Walace |
dc.subject.por.fl_str_mv |
Bean Germination Moringa Vigor. Feijão Germinação Moringa Vigor. |
topic |
Bean Germination Moringa Vigor. Feijão Germinação Moringa Vigor. |
description |
In order to investigate the effect of different doses of essential oil of Moringa oleifera (EOM) on physiological potential and the expression of isoenzymes in bean seeds, an experiment was conducted with two cultivars (Colibri and Campos Gerais) and seven treatments (bactericidal, fungicide and the doses 0, 0.1, 0.2, 0.4 and 0.8% EOM). Germination, first count of germination, germination speed index (GSI), emergence and emergence rate index (ERI) were evaluated in addition to the enzymes esterase (EST), alcohol dehydrogenase (ADH), superoxide dismutase (SOD) and isocitrate lyase (ICL) by electrophoresis. In an experiment conducted in the laboratory, the dose containing 0.4% EOM promoted germination and higher averages for first germination in seeds and GSI in the Colibri cultivar. However, the highest dose used (0.8%) was responsible for the worst physiological performance by the cultivar. For Campos Gerais, increasing doses of essential oil stimulated germination, first count of germination and GSI. In the field, higher doses of essential oil gave higher germination percentages for the Colibri cultivar, not influencing the seeds of Campos Gerais. The ERI was not altered by the treatments with OEM and changes in the molecular forms of the isoenzymes were observed, with low expression values of bands for EST, ICL and ADH-related vigor, the germination of both cultivars, as well as increased expression of SOD, which is associated with reductions in germination. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20921 10.5433/1679-0359.2016v37n2p625 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20921 |
identifier_str_mv |
10.5433/1679-0359.2016v37n2p625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20921/18583 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 2 (2016); 625-638 Semina: Ciências Agrárias; v. 37 n. 2 (2016); 625-638 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306073363972096 |