Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620 |
Resumo: | Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. |
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Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrificationMangabeirarecalcitrant specieslong-term conservationdehydration.Botânica Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. Universidade Estadual De Maringá2017-05-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfimage/jpegimage/jpegimage/jpegapplication/zipapplication/pdfapplication/mswordapplication/pdfapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3162010.4025/actascibiolsci.v39i1.31620Acta Scientiarum. Biological Sciences; Vol 39 No 1 (2017); 87-93Acta Scientiarum. Biological Sciences; v. 39 n. 1 (2017); 87-931807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMengporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144687http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144688http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144689http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144690http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144691http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145552http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145553http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145554http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145555Copyright (c) 2017 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessPrudente, Débora de OliveiraPaiva, RenatoNery, Fernanda CarlotaMáximo, Wesley Pires FlausinoSilva, Luciano Coutinho2022-02-20T22:00:08Zoai:periodicos.uem.br/ojs:article/31620Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-20T22:00:08Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
title |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
spellingShingle |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification Prudente, Débora de Oliveira Mangabeira recalcitrant species long-term conservation dehydration. Botânica |
title_short |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
title_full |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
title_fullStr |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
title_full_unstemmed |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
title_sort |
Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
author |
Prudente, Débora de Oliveira |
author_facet |
Prudente, Débora de Oliveira Paiva, Renato Nery, Fernanda Carlota Máximo, Wesley Pires Flausino Silva, Luciano Coutinho |
author_role |
author |
author2 |
Paiva, Renato Nery, Fernanda Carlota Máximo, Wesley Pires Flausino Silva, Luciano Coutinho |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Prudente, Débora de Oliveira Paiva, Renato Nery, Fernanda Carlota Máximo, Wesley Pires Flausino Silva, Luciano Coutinho |
dc.subject.por.fl_str_mv |
Mangabeira recalcitrant species long-term conservation dehydration. Botânica |
topic |
Mangabeira recalcitrant species long-term conservation dehydration. Botânica |
description |
Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620 10.4025/actascibiolsci.v39i1.31620 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620 |
identifier_str_mv |
10.4025/actascibiolsci.v39i1.31620 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144687 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144688 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144689 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144690 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375144691 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145552 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145553 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145554 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31620/751375145555 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf image/jpeg image/jpeg image/jpeg application/zip application/pdf application/msword application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 39 No 1 (2017); 87-93 Acta Scientiarum. Biological Sciences; v. 39 n. 1 (2017); 87-93 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317396471676928 |