Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827 |
Resumo: | Nowadays, the isolation of new bacterial strains that produce enzymes with novel properties is a subject of great relevance to the scientific community. This study, in order to search for producers of new cellulase strains, investigated the avicelase production by thermophilic Bacillus sp. strain SMIA-2. The best avicelase activity was observed in a culture medium containing 0.5% (w v-1) avicel and 0.5% (w v-1) corn steep liquor with initial pH 7.5-8.0 incubated at 50oC. When avicel was replaced in the medium by the treated sugarcane bagasse (0.5%, w v-1) the avicelase activity levels were not affected. Studies on the avicelase characterization revealed that the optimum pH of the enzyme was found to be 8.5 and the enzyme retained more than 80% of its activity after incubation at room temperature for 2h at pH 6.5-8.5. The optimum temperature of this enzyme was 70oC and the enzyme retained 67% of the original activity after 20 min. of heat treatment at 70oC. Avicelase was stimulated by Mn2+ and Co2+, whereas Hg2+ greatly inhibited the enzyme activity |
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Acta Scientiarum Biological Sciences |
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Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2Avicelasecorn steep liquorsugarcane bagasseBacillus spTecnologia de AlimentosNowadays, the isolation of new bacterial strains that produce enzymes with novel properties is a subject of great relevance to the scientific community. This study, in order to search for producers of new cellulase strains, investigated the avicelase production by thermophilic Bacillus sp. strain SMIA-2. The best avicelase activity was observed in a culture medium containing 0.5% (w v-1) avicel and 0.5% (w v-1) corn steep liquor with initial pH 7.5-8.0 incubated at 50oC. When avicel was replaced in the medium by the treated sugarcane bagasse (0.5%, w v-1) the avicelase activity levels were not affected. Studies on the avicelase characterization revealed that the optimum pH of the enzyme was found to be 8.5 and the enzyme retained more than 80% of its activity after incubation at room temperature for 2h at pH 6.5-8.5. The optimum temperature of this enzyme was 70oC and the enzyme retained 67% of the original activity after 20 min. of heat treatment at 70oC. Avicelase was stimulated by Mn2+ and Co2+, whereas Hg2+ greatly inhibited the enzyme activity Universidade Estadual De Maringá2014-05-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1782710.4025/actascibiolsci.v36i2.17827Acta Scientiarum. Biological Sciences; Vol 36 No 2 (2014); 215-222Acta Scientiarum. Biological Sciences; v. 36 n. 2 (2014); 215-2221807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827/pdf_12Oliveira, Luciana Ribeiro CoutinhoBarbosa, João BatistaMartins, Meire Lelis LealMartins, Marco Antonioinfo:eu-repo/semantics/openAccess2014-05-08T16:16:33Zoai:periodicos.uem.br/ojs:article/17827Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2014-05-08T16:16:33Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
title |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
spellingShingle |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 Oliveira, Luciana Ribeiro Coutinho Avicelase corn steep liquor sugarcane bagasse Bacillus sp Tecnologia de Alimentos |
title_short |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
title_full |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
title_fullStr |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
title_full_unstemmed |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
title_sort |
Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 |
author |
Oliveira, Luciana Ribeiro Coutinho |
author_facet |
Oliveira, Luciana Ribeiro Coutinho Barbosa, João Batista Martins, Meire Lelis Leal Martins, Marco Antonio |
author_role |
author |
author2 |
Barbosa, João Batista Martins, Meire Lelis Leal Martins, Marco Antonio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Luciana Ribeiro Coutinho Barbosa, João Batista Martins, Meire Lelis Leal Martins, Marco Antonio |
dc.subject.por.fl_str_mv |
Avicelase corn steep liquor sugarcane bagasse Bacillus sp Tecnologia de Alimentos |
topic |
Avicelase corn steep liquor sugarcane bagasse Bacillus sp Tecnologia de Alimentos |
description |
Nowadays, the isolation of new bacterial strains that produce enzymes with novel properties is a subject of great relevance to the scientific community. This study, in order to search for producers of new cellulase strains, investigated the avicelase production by thermophilic Bacillus sp. strain SMIA-2. The best avicelase activity was observed in a culture medium containing 0.5% (w v-1) avicel and 0.5% (w v-1) corn steep liquor with initial pH 7.5-8.0 incubated at 50oC. When avicel was replaced in the medium by the treated sugarcane bagasse (0.5%, w v-1) the avicelase activity levels were not affected. Studies on the avicelase characterization revealed that the optimum pH of the enzyme was found to be 8.5 and the enzyme retained more than 80% of its activity after incubation at room temperature for 2h at pH 6.5-8.5. The optimum temperature of this enzyme was 70oC and the enzyme retained 67% of the original activity after 20 min. of heat treatment at 70oC. Avicelase was stimulated by Mn2+ and Co2+, whereas Hg2+ greatly inhibited the enzyme activity |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827 10.4025/actascibiolsci.v36i2.17827 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827 |
identifier_str_mv |
10.4025/actascibiolsci.v36i2.17827 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827/pdf_12 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 36 No 2 (2014); 215-222 Acta Scientiarum. Biological Sciences; v. 36 n. 2 (2014); 215-222 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317395585630208 |