Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
Resumo: | By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate. |
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Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777creole frogGnRHasemenspermatozoonsperm motilityAquiculturaBy understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate. Universidade Estadual De Maringá2012-11-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionestudos morfológicosapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1477710.4025/actascibiolsci.v35i1.14777Acta Scientiarum. Biological Sciences; Vol 35 No 1 (2013); 1-7Acta Scientiarum. Biological Sciences; v. 35 n. 1 (2013); 1-71807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777/pdf_1Pereira, Marcelo MaiaRibeiro Filho, Oswaldo PintoZanuncio, Jose ColaTroni, Allan ReisNavarro, Rodrigo DianaTakamura, Angela Emiinfo:eu-repo/semantics/openAccess2022-11-23T17:34:08Zoai:periodicos.uem.br/ojs:article/14777Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:34:08Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
title |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
spellingShingle |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 Pereira, Marcelo Maia creole frog GnRHa semen spermatozoon sperm motility Aquicultura |
title_short |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
title_full |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
title_fullStr |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
title_full_unstemmed |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
title_sort |
Buserelin acetate-induced spermiation in Leptodactylus ocellatus and evaluation of semen characteristics - doi: 10.4025/actascibiolsci.v35i1.14777 |
author |
Pereira, Marcelo Maia |
author_facet |
Pereira, Marcelo Maia Ribeiro Filho, Oswaldo Pinto Zanuncio, Jose Cola Troni, Allan Reis Navarro, Rodrigo Diana Takamura, Angela Emi |
author_role |
author |
author2 |
Ribeiro Filho, Oswaldo Pinto Zanuncio, Jose Cola Troni, Allan Reis Navarro, Rodrigo Diana Takamura, Angela Emi |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Marcelo Maia Ribeiro Filho, Oswaldo Pinto Zanuncio, Jose Cola Troni, Allan Reis Navarro, Rodrigo Diana Takamura, Angela Emi |
dc.subject.por.fl_str_mv |
creole frog GnRHa semen spermatozoon sperm motility Aquicultura |
topic |
creole frog GnRHa semen spermatozoon sperm motility Aquicultura |
description |
By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion estudos morfológicos |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 10.4025/actascibiolsci.v35i1.14777 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
identifier_str_mv |
10.4025/actascibiolsci.v35i1.14777 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 35 No 1 (2013); 1-7 Acta Scientiarum. Biological Sciences; v. 35 n. 1 (2013); 1-7 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1750312452643356672 |