Proceedings to avoid contaminants during isolation and storage of standard fungal strains
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Publication Date: | 2008 |
Other Authors: | , , , |
Format: | Article |
Language: | por |
Source: | Acta Scientiarum Biological Sciences |
Download full: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2831 |
Summary: | The fungal strains for scientific purposes are isolated from environments, where interaction of extensive taxonomical groups is a common phenomenon, requiring development of a method that assures elimination of microbial interference and preserves original characteristic of strains. In this study, using Fusarium strains isolated from corn and animal food, the interference of antibiotic resistant bacteria and yeast contamination in the fungal strains storage was evaluated. Although frequent contamination by tetracycline, streptomycine and chloramphenicol resistant microorganisms was detected in both corn and animal food, tetracycline showed the best antibacterial effect. Following 6 months storage at 4ºC, 36 cultures of 168 single spored Fusarium strains developed atypical colonies, indicating some contamination. By plating these cultures in BDA medium added with antibiotic, 21 strains were purified, but the technique was unable to reisolate the remaining ones. The purity of these 15 strains was recuperated using dilution/plate technique proposed here, which used combination of dilution process followed by plating in BDA medium surface. The procedure is recommended as the final step for fungal strains recuperation, which allows elimination of fast growing undesirable organisms |
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Proceedings to avoid contaminants during isolation and storage of standard fungal strainsProcessos de eliminação de contaminantes microbianos durante o isolamento e a manutenção de linhagens fúngicas de referênciaFusarium moniliformeisolamentomanutenção de linhagens fúngicas2.00.00.00-6 Ciências BiológicasThe fungal strains for scientific purposes are isolated from environments, where interaction of extensive taxonomical groups is a common phenomenon, requiring development of a method that assures elimination of microbial interference and preserves original characteristic of strains. In this study, using Fusarium strains isolated from corn and animal food, the interference of antibiotic resistant bacteria and yeast contamination in the fungal strains storage was evaluated. Although frequent contamination by tetracycline, streptomycine and chloramphenicol resistant microorganisms was detected in both corn and animal food, tetracycline showed the best antibacterial effect. Following 6 months storage at 4ºC, 36 cultures of 168 single spored Fusarium strains developed atypical colonies, indicating some contamination. By plating these cultures in BDA medium added with antibiotic, 21 strains were purified, but the technique was unable to reisolate the remaining ones. The purity of these 15 strains was recuperated using dilution/plate technique proposed here, which used combination of dilution process followed by plating in BDA medium surface. The procedure is recommended as the final step for fungal strains recuperation, which allows elimination of fast growing undesirable organismsLinhagens fúngicas destinadas a pesquisa científica são provenientes de material orgânico, onde ocorre intensa interação entre os mais variados grupos taxonômicos de microrganismos. O desenvolvimento de uma metodologia capaz de remover totalmente esses contaminantes indesejáveis é fundamental para garantir a característica original da linhagem em estudo. Empregando-se isolados de Fusarium spp., obtidos de milho e rações animais, analisou-se a interferência de bactérias resistentes a antibióticos e leveduras na manutenção de linhagens fúngicas. O ensaio demonstrou contaminação freqüente de microrganismos resistentes a tetraciclina, estreptomicina e cloranfenicol na microbiota natural de milho e de rações animais. O melhor controle dos interferentes foi obtido com tetraciclina, segundo avaliação por replica-plate e crescimento em meio líquido. Na análise de 168 linhagens de Fusarium spp. mantidos por seis meses a 4ºC, 36 cepas apresentaram contaminantes. A técnica de esgotamento em BDA mais antibiótico repurificou 21 cepas. As 15 cepas restantes só foram repurificadas pela técnica de diluição/plaqueamento, desenvolvida neste trabalho e recomendada para ser utilizada como último recurso na recuperação de linhagens de referências contaminadasUniversidade Estadual De Maringá2008-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/283110.4025/actascibiolsci.v22i0.2831Acta Scientiarum. Biological Sciences; Vol 22 (2000); 321-326Acta Scientiarum. Biological Sciences; v. 22 (2000); 321-3261807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2831/2096Figueira, Edson Luiz ZangrandoVicente, EduardoYoshimoto, YukoOno, Elizabeth Yurie SatakeHirooka, Elisa Yokoinfo:eu-repo/semantics/openAccess2022-11-23T17:33:30Zoai:periodicos.uem.br/ojs:article/2831Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:30Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains Processos de eliminação de contaminantes microbianos durante o isolamento e a manutenção de linhagens fúngicas de referência |
title |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains |
spellingShingle |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains Figueira, Edson Luiz Zangrando Fusarium moniliforme isolamento manutenção de linhagens fúngicas 2.00.00.00-6 Ciências Biológicas |
title_short |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains |
title_full |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains |
title_fullStr |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains |
title_full_unstemmed |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains |
title_sort |
Proceedings to avoid contaminants during isolation and storage of standard fungal strains |
author |
Figueira, Edson Luiz Zangrando |
author_facet |
Figueira, Edson Luiz Zangrando Vicente, Eduardo Yoshimoto, Yuko Ono, Elizabeth Yurie Satake Hirooka, Elisa Yoko |
author_role |
author |
author2 |
Vicente, Eduardo Yoshimoto, Yuko Ono, Elizabeth Yurie Satake Hirooka, Elisa Yoko |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Figueira, Edson Luiz Zangrando Vicente, Eduardo Yoshimoto, Yuko Ono, Elizabeth Yurie Satake Hirooka, Elisa Yoko |
dc.subject.por.fl_str_mv |
Fusarium moniliforme isolamento manutenção de linhagens fúngicas 2.00.00.00-6 Ciências Biológicas |
topic |
Fusarium moniliforme isolamento manutenção de linhagens fúngicas 2.00.00.00-6 Ciências Biológicas |
description |
The fungal strains for scientific purposes are isolated from environments, where interaction of extensive taxonomical groups is a common phenomenon, requiring development of a method that assures elimination of microbial interference and preserves original characteristic of strains. In this study, using Fusarium strains isolated from corn and animal food, the interference of antibiotic resistant bacteria and yeast contamination in the fungal strains storage was evaluated. Although frequent contamination by tetracycline, streptomycine and chloramphenicol resistant microorganisms was detected in both corn and animal food, tetracycline showed the best antibacterial effect. Following 6 months storage at 4ºC, 36 cultures of 168 single spored Fusarium strains developed atypical colonies, indicating some contamination. By plating these cultures in BDA medium added with antibiotic, 21 strains were purified, but the technique was unable to reisolate the remaining ones. The purity of these 15 strains was recuperated using dilution/plate technique proposed here, which used combination of dilution process followed by plating in BDA medium surface. The procedure is recommended as the final step for fungal strains recuperation, which allows elimination of fast growing undesirable organisms |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-05-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2831 10.4025/actascibiolsci.v22i0.2831 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2831 |
identifier_str_mv |
10.4025/actascibiolsci.v22i0.2831 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2831/2096 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 22 (2000); 321-326 Acta Scientiarum. Biological Sciences; v. 22 (2000); 321-326 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317393509449728 |