AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions

Detalhes bibliográficos
Autor(a) principal: Andrade, Felipe Aranha de
Data de Publicação: 2016
Outros Autores: Gonçalves, Leandro Simões Azeredo, Miglioranza, Édison, Ruas, Claudete de Fátima, Ruas, Paulo Mauricio, Takahashi, Lúcia Sadayo Assari
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577
Resumo: The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair Cluster Method with Arithmetic Mean (UPGMA), principal coordinates analysis (PCoA) and Bayesian statistics were used for the clustering analysis. The three combinations of primer/enzyme revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. 
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spelling AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessionsAFLP analysis of genetic diversity in determinate and indeterminate snap bean accessionsPhaseolus vulgaris L.molecular markersAmplified Fragment Length Polymorphismgene bankgenetic diversityRecursos GenéticosPhaseolus vulgaris L.molecular markersamplified fragment length polymorphismgene bankThe present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair Cluster Method with Arithmetic Mean (UPGMA), principal coordinates analysis (PCoA) and Bayesian statistics were used for the clustering analysis. The three combinations of primer/enzyme revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. These selective AFLP combinations revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. Universidade Estadual de Maringá2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/2557710.4025/actasciagron.v38i1.25577Acta Scientiarum. Agronomy; Vol 38 No 1 (2016); 29-34Acta Scientiarum. Agronomy; v. 38 n. 1 (2016); 29-341807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/pdf_110http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/751375143394Andrade, Felipe Aranha deGonçalves, Leandro Simões AzeredoMiglioranza, ÉdisonRuas, Claudete de FátimaRuas, Paulo MauricioTakahashi, Lúcia Sadayo Assariinfo:eu-repo/semantics/openAccess2016-02-17T15:35:41Zoai:periodicos.uem.br/ojs:article/25577Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2016-02-17T15:35:41Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
title AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
spellingShingle AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
Andrade, Felipe Aranha de
Phaseolus vulgaris L.
molecular markers
Amplified Fragment Length Polymorphism
gene bank
genetic diversity
Recursos Genéticos
Phaseolus vulgaris L.
molecular markers
amplified fragment length polymorphism
gene bank
title_short AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
title_full AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
title_fullStr AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
title_full_unstemmed AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
title_sort AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
author Andrade, Felipe Aranha de
author_facet Andrade, Felipe Aranha de
Gonçalves, Leandro Simões Azeredo
Miglioranza, Édison
Ruas, Claudete de Fátima
Ruas, Paulo Mauricio
Takahashi, Lúcia Sadayo Assari
author_role author
author2 Gonçalves, Leandro Simões Azeredo
Miglioranza, Édison
Ruas, Claudete de Fátima
Ruas, Paulo Mauricio
Takahashi, Lúcia Sadayo Assari
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Felipe Aranha de
Gonçalves, Leandro Simões Azeredo
Miglioranza, Édison
Ruas, Claudete de Fátima
Ruas, Paulo Mauricio
Takahashi, Lúcia Sadayo Assari
dc.subject.por.fl_str_mv Phaseolus vulgaris L.
molecular markers
Amplified Fragment Length Polymorphism
gene bank
genetic diversity
Recursos Genéticos
Phaseolus vulgaris L.
molecular markers
amplified fragment length polymorphism
gene bank
topic Phaseolus vulgaris L.
molecular markers
Amplified Fragment Length Polymorphism
gene bank
genetic diversity
Recursos Genéticos
Phaseolus vulgaris L.
molecular markers
amplified fragment length polymorphism
gene bank
description The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair Cluster Method with Arithmetic Mean (UPGMA), principal coordinates analysis (PCoA) and Bayesian statistics were used for the clustering analysis. The three combinations of primer/enzyme revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatest number of informative loci (49%). The analysis of dissimilarity frequency distribution showed that the distribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions with indeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. The clustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is a possible association between snap bean growth habit and gene pool. The determinate accessions may be more closely associated with the Andean gene pool, while the indeterminate ones may be associated with the Mesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggesting introgression between the Andean and Mesoamerican gene pools. 
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577
10.4025/actasciagron.v38i1.25577
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577
identifier_str_mv 10.4025/actasciagron.v38i1.25577
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/pdf_110
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577/751375143394
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 38 No 1 (2016); 29-34
Acta Scientiarum. Agronomy; v. 38 n. 1 (2016); 29-34
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
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