Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473

Detalhes bibliográficos
Autor(a) principal: Vieira, José Geraldo Zaparoli
Data de Publicação: 2008
Outros Autores: Faria, Ricardo Tadeu de, Vieira, Luiz Gonzaga Esteves, Molinari, Hugo Bruno Correa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1473
Resumo: A transformation protocol using mannose as selective agent was evaluated for genetic transformation of the rootstock Swingle citrumelo. Two independent experiments were carried out to define the smallest mannose concentration capable to inhibit the multiplication of adventitious buds. Based on these results, 5.0 mM of mannose was chosen for Swingle citrumelo genetic transformation. An 11% transformation efficiency was obtained using the Agrobacterium tumefaciens binary strain EHA105. The results described in this study showed that, despite the relatively low transformation frequency, the gene pmi is a good alternative to the most commonly used selection scheme for citrus transformation, based on gene nptII for kanamycin resistance.
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spelling Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473Transformação genética de citrumelo Swingle (Citrus paradisi Macf. × Poncirus trifoliata L. Raf.) com o gene marcador fosfomanose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473Citrusmanosetransformação genéticagene marcadorfosfomanose isomerase5.01.00.00-9 AgronomiaA transformation protocol using mannose as selective agent was evaluated for genetic transformation of the rootstock Swingle citrumelo. Two independent experiments were carried out to define the smallest mannose concentration capable to inhibit the multiplication of adventitious buds. Based on these results, 5.0 mM of mannose was chosen for Swingle citrumelo genetic transformation. An 11% transformation efficiency was obtained using the Agrobacterium tumefaciens binary strain EHA105. The results described in this study showed that, despite the relatively low transformation frequency, the gene pmi is a good alternative to the most commonly used selection scheme for citrus transformation, based on gene nptII for kanamycin resistance.Um protocolo de transformação usando manose como agente seletivo foi avaliado para transformação genética do porta-enxerto citrumelo Swingle. Dois experimentos independentes foram conduzidos visando definir a curva de resposta para determinar a menor concentração de manose capaz de inibir a multiplicação de brotos. Baseando-se nesses resultados, 5,0 mM de manose foi utilizado para a transformação genética de citrumelo Swingle. Plantas transgênicas foram obtidas via Agrobacterium tumefaciens, estirpe EHA105, com uma eficiência de transformação de 11%. Os resultados descritos neste estudo demonstram que apesar da freqüência de transformação ainda ser considerada relativamente baixa, o gene pmi mostra-se como uma boa alternativa ao sistema de seleção mais comumente empregado para a transformação de citros baseado no gene nptII para resistência a canamicina.Universidade Estadual de Maringá2008-03-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/147310.4025/actasciagron.v27i3.1473Acta Scientiarum. Agronomy; Vol 27 No 3 (2005); 541-547Acta Scientiarum. Agronomy; v. 27 n. 3 (2005); 541-5471807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1473/832Vieira, José Geraldo ZaparoliFaria, Ricardo Tadeu deVieira, Luiz Gonzaga EstevesMolinari, Hugo Bruno Correainfo:eu-repo/semantics/openAccess2022-11-23T18:37:26Zoai:periodicos.uem.br/ojs:article/1473Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:37:26Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
Transformação genética de citrumelo Swingle (Citrus paradisi Macf. × Poncirus trifoliata L. Raf.) com o gene marcador fosfomanose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
title Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
spellingShingle Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
Vieira, José Geraldo Zaparoli
Citrus
manose
transformação genética
gene marcador
fosfomanose isomerase
5.01.00.00-9 Agronomia
title_short Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
title_full Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
title_fullStr Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
title_full_unstemmed Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
title_sort Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473
author Vieira, José Geraldo Zaparoli
author_facet Vieira, José Geraldo Zaparoli
Faria, Ricardo Tadeu de
Vieira, Luiz Gonzaga Esteves
Molinari, Hugo Bruno Correa
author_role author
author2 Faria, Ricardo Tadeu de
Vieira, Luiz Gonzaga Esteves
Molinari, Hugo Bruno Correa
author2_role author
author
author
dc.contributor.author.fl_str_mv Vieira, José Geraldo Zaparoli
Faria, Ricardo Tadeu de
Vieira, Luiz Gonzaga Esteves
Molinari, Hugo Bruno Correa
dc.subject.por.fl_str_mv Citrus
manose
transformação genética
gene marcador
fosfomanose isomerase
5.01.00.00-9 Agronomia
topic Citrus
manose
transformação genética
gene marcador
fosfomanose isomerase
5.01.00.00-9 Agronomia
description A transformation protocol using mannose as selective agent was evaluated for genetic transformation of the rootstock Swingle citrumelo. Two independent experiments were carried out to define the smallest mannose concentration capable to inhibit the multiplication of adventitious buds. Based on these results, 5.0 mM of mannose was chosen for Swingle citrumelo genetic transformation. An 11% transformation efficiency was obtained using the Agrobacterium tumefaciens binary strain EHA105. The results described in this study showed that, despite the relatively low transformation frequency, the gene pmi is a good alternative to the most commonly used selection scheme for citrus transformation, based on gene nptII for kanamycin resistance.
publishDate 2008
dc.date.none.fl_str_mv 2008-03-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1473
10.4025/actasciagron.v27i3.1473
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1473
identifier_str_mv 10.4025/actasciagron.v27i3.1473
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1473/832
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 27 No 3 (2005); 541-547
Acta Scientiarum. Agronomy; v. 27 n. 3 (2005); 541-547
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
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