Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/58336 |
Resumo: | Banana (Musa spp.) is one of the main fruits consumed worldwide. However, finger drop, is a physiological disorder that causes many postharvest problems, which eventually reduces market value and consumer acceptance. Therefore, the objective of the study was to evaluate the anatomical changes that occur in the pedicel rupture area (drop zone) of bananas diploids (BB França) and tetraploid (BRS Pioneira) in three ripening stages. The levels of gene expression involved in the natural ripening process and in the development of finger drop, was also investigated. The accumulation of their mRNAs and those of expansin (EXP1), pectate lyase (PEL1) and xyloglucan endotransglucosylase/hydrolase protein (XTH4) genes already isolated from bananas were measured by quantitative polymerase chain reaction in three ripening stages. BB França presented a higher resistance to finger drop due to the presence of some specific morphoanatomical characteristics, such as larger parenchymal cells and greater deposition of lignin. In contrast, there was degeneration of the pedicel parenchymal tissue of the BRS Pioneira genotype, forming large empty spaces during the ripening of the fruits, mainly in stage 6, which contributed to the finger drop. The diploid BB França is a strong candidate for use in banana breeding programs aimed at fruit drop resistance. This will certainly improve the quality of banana varieties. Moreover, PEL1 proved to be an excellent candidate gene for functional studies of finger drop in bananas. |
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Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananasMorpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananasMusaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR.Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR.Banana (Musa spp.) is one of the main fruits consumed worldwide. However, finger drop, is a physiological disorder that causes many postharvest problems, which eventually reduces market value and consumer acceptance. Therefore, the objective of the study was to evaluate the anatomical changes that occur in the pedicel rupture area (drop zone) of bananas diploids (BB França) and tetraploid (BRS Pioneira) in three ripening stages. The levels of gene expression involved in the natural ripening process and in the development of finger drop, was also investigated. The accumulation of their mRNAs and those of expansin (EXP1), pectate lyase (PEL1) and xyloglucan endotransglucosylase/hydrolase protein (XTH4) genes already isolated from bananas were measured by quantitative polymerase chain reaction in three ripening stages. BB França presented a higher resistance to finger drop due to the presence of some specific morphoanatomical characteristics, such as larger parenchymal cells and greater deposition of lignin. In contrast, there was degeneration of the pedicel parenchymal tissue of the BRS Pioneira genotype, forming large empty spaces during the ripening of the fruits, mainly in stage 6, which contributed to the finger drop. The diploid BB França is a strong candidate for use in banana breeding programs aimed at fruit drop resistance. This will certainly improve the quality of banana varieties. Moreover, PEL1 proved to be an excellent candidate gene for functional studies of finger drop in bananas.Banana (Musa spp.) is one of the main fruits consumed worldwide. However, finger drop, is a physiological disorder that causes many postharvest problems, which eventually reduces market value and consumer acceptance. Therefore, the objective of the study was to evaluate the anatomical changes that occur in the pedicel rupture area (drop zone) of bananas diploids (BB França) and tetraploid (BRS Pioneira) in three ripening stages. The levels of gene expression involved in the natural ripening process and in the development of finger drop, was also investigated. The accumulation of their mRNAs and those of expansin (EXP1), pectate lyase (PEL1) and xyloglucan endotransglucosylase/hydrolase protein (XTH4) genes already isolated from bananas were measured by quantitative polymerase chain reaction in three ripening stages. BB França presented a higher resistance to finger drop due to the presence of some specific morphoanatomical characteristics, such as larger parenchymal cells and greater deposition of lignin. In contrast, there was degeneration of the pedicel parenchymal tissue of the BRS Pioneira genotype, forming large empty spaces during the ripening of the fruits, mainly in stage 6, which contributed to the finger drop. The diploid BB França is a strong candidate for use in banana breeding programs aimed at fruit drop resistance. This will certainly improve the quality of banana varieties. Moreover, PEL1 proved to be an excellent candidate gene for functional studies of finger drop in bananas.Universidade Estadual de Maringá2023-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5833610.4025/actasciagron.v45i1.58336Acta Scientiarum. Agronomy; Vol 45 (2023): Publicação contínua; e58336Acta Scientiarum. Agronomy; v. 45 (2023): Publicação contínua; e583361807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/58336/751375155606Copyright (c) 2023 Acta Scientiarum. Agronomyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, Marciene AmorimFerreira , Claudia Fortes Amorim, Edson PeritoSantos, Rogério Merces Ferreira Martins, Fabiano MachadoLedo , Carlos Alberto da Silva Soares, Taliane LeilaSantana , José Raniere Ferreira de2023-04-24T18:26:17Zoai:periodicos.uem.br/ojs:article/58336Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2023-04-24T18:26:17Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
title |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
spellingShingle |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas Rodrigues, Marciene Amorim Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR. Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR. |
title_short |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
title_full |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
title_fullStr |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
title_full_unstemmed |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
title_sort |
Morpho-anatomical characterization, gene expression and protein cell wall modifications associated with natural finger drop in bananas |
author |
Rodrigues, Marciene Amorim |
author_facet |
Rodrigues, Marciene Amorim Ferreira , Claudia Fortes Amorim, Edson Perito Santos, Rogério Merces Ferreira Martins, Fabiano Machado Ledo , Carlos Alberto da Silva Soares, Taliane Leila Santana , José Raniere Ferreira de |
author_role |
author |
author2 |
Ferreira , Claudia Fortes Amorim, Edson Perito Santos, Rogério Merces Ferreira Martins, Fabiano Machado Ledo , Carlos Alberto da Silva Soares, Taliane Leila Santana , José Raniere Ferreira de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Marciene Amorim Ferreira , Claudia Fortes Amorim, Edson Perito Santos, Rogério Merces Ferreira Martins, Fabiano Machado Ledo , Carlos Alberto da Silva Soares, Taliane Leila Santana , José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR. Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR. |
topic |
Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR. Musaceae; cell wall; fruit drop; anatomical analysis; RT-qPCR. |
description |
Banana (Musa spp.) is one of the main fruits consumed worldwide. However, finger drop, is a physiological disorder that causes many postharvest problems, which eventually reduces market value and consumer acceptance. Therefore, the objective of the study was to evaluate the anatomical changes that occur in the pedicel rupture area (drop zone) of bananas diploids (BB França) and tetraploid (BRS Pioneira) in three ripening stages. The levels of gene expression involved in the natural ripening process and in the development of finger drop, was also investigated. The accumulation of their mRNAs and those of expansin (EXP1), pectate lyase (PEL1) and xyloglucan endotransglucosylase/hydrolase protein (XTH4) genes already isolated from bananas were measured by quantitative polymerase chain reaction in three ripening stages. BB França presented a higher resistance to finger drop due to the presence of some specific morphoanatomical characteristics, such as larger parenchymal cells and greater deposition of lignin. In contrast, there was degeneration of the pedicel parenchymal tissue of the BRS Pioneira genotype, forming large empty spaces during the ripening of the fruits, mainly in stage 6, which contributed to the finger drop. The diploid BB França is a strong candidate for use in banana breeding programs aimed at fruit drop resistance. This will certainly improve the quality of banana varieties. Moreover, PEL1 proved to be an excellent candidate gene for functional studies of finger drop in bananas. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/58336 10.4025/actasciagron.v45i1.58336 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/58336 |
identifier_str_mv |
10.4025/actasciagron.v45i1.58336 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/58336/751375155606 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 45 (2023): Publicação contínua; e58336 Acta Scientiarum. Agronomy; v. 45 (2023): Publicação contínua; e58336 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
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1799305901209812992 |