Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332 |
Resumo: | Triazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics. |
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Acta Scientiarum. Agronomy (Online) |
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Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazilblast diseaseCYP51A genesterol demethylation inhibitor (DMI) fungicidesepoxiconazoletebuconazoleResistência a fungicidas na agricultura / Fungicide resistance in agricultureTriazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics.Universidade Estadual de Maringá2019-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa experimental de laboratórioapplication/pdfimage/jpegimage/jpegimage/jpeghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3933210.4025/actasciagron.v41i1.39332Acta Scientiarum. Agronomy; Vol 41 (2019): Publicação Contínua; e39332Acta Scientiarum. Agronomy; v. 41 (2019): Publicação Contínua; e393321807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/751375146266http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/751375146267http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/751375146269Copyright (c) 2019 Acta Scientiarum. Agronomyhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDorigan, Adriano FrancisCarvalho, Giselle dePoloni, Nadia MariaNegrisoli, Matheus MerebMaciel, João Leodato NunesCeresini, Paulo Cezar2019-09-24T12:25:27Zoai:periodicos.uem.br/ojs:article/39332Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2019-09-24T12:25:27Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
title |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
spellingShingle |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil Dorigan, Adriano Francis blast disease CYP51A gene sterol demethylation inhibitor (DMI) fungicides epoxiconazole tebuconazole Resistência a fungicidas na agricultura / Fungicide resistance in agriculture |
title_short |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
title_full |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
title_fullStr |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
title_full_unstemmed |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
title_sort |
Resistance to triazole fungicides in Pyricularia species associated with invasive plants from wheat fields in Brazil |
author |
Dorigan, Adriano Francis |
author_facet |
Dorigan, Adriano Francis Carvalho, Giselle de Poloni, Nadia Maria Negrisoli, Matheus Mereb Maciel, João Leodato Nunes Ceresini, Paulo Cezar |
author_role |
author |
author2 |
Carvalho, Giselle de Poloni, Nadia Maria Negrisoli, Matheus Mereb Maciel, João Leodato Nunes Ceresini, Paulo Cezar |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dorigan, Adriano Francis Carvalho, Giselle de Poloni, Nadia Maria Negrisoli, Matheus Mereb Maciel, João Leodato Nunes Ceresini, Paulo Cezar |
dc.subject.por.fl_str_mv |
blast disease CYP51A gene sterol demethylation inhibitor (DMI) fungicides epoxiconazole tebuconazole Resistência a fungicidas na agricultura / Fungicide resistance in agriculture |
topic |
blast disease CYP51A gene sterol demethylation inhibitor (DMI) fungicides epoxiconazole tebuconazole Resistência a fungicidas na agricultura / Fungicide resistance in agriculture |
description |
Triazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa experimental de laboratório |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332 10.4025/actasciagron.v41i1.39332 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332 |
identifier_str_mv |
10.4025/actasciagron.v41i1.39332 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/751375146266 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/751375146267 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39332/751375146269 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Acta Scientiarum. Agronomy https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf image/jpeg image/jpeg image/jpeg |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 41 (2019): Publicação Contínua; e39332 Acta Scientiarum. Agronomy; v. 41 (2019): Publicação Contínua; e39332 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
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1799305910516973568 |