Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342 |
Resumo: | The development of an efficient regeneration system is crucial for the genetic transformation of sugarcane. The aims of this study were to regenerate plants from somatic embryos and analyze the origin and division pattern of cells during the different embryonic developmental stages of the sugarcane cultivars RB855156 and RB72454. For both cultivars, the best results for embryogenic callus induction were obtained with explant cultures on culture medium containing 13.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by callus culture on solid medium with 4.5 µM 2,4-D. A higher rate of shoot induction was observed in RB855156 with the addition of 8.9 and 17.8 µM benzylaminopurine (BAP); this higher rate was also observed in RB72454 with the addition of 17.8 µM BAP. Murashige and Skoog (1962) (MS) medium containing 2.5 and 5.0 µM indolebutyric acid (IBA) for RB855156 and RB72454, respectively, was suitable for rooting. The plantlets were successfully acclimatized and the plant survival rate was 100% for both cultivars. Histological analysis revealed that the shoots in both cultivars originated from somatic embryogenesis |
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Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342tissue culturesomatic embryosanatomy24-DPoaceaeCiências AgráriasFitotecniaMelhoramento VegetalThe development of an efficient regeneration system is crucial for the genetic transformation of sugarcane. The aims of this study were to regenerate plants from somatic embryos and analyze the origin and division pattern of cells during the different embryonic developmental stages of the sugarcane cultivars RB855156 and RB72454. For both cultivars, the best results for embryogenic callus induction were obtained with explant cultures on culture medium containing 13.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by callus culture on solid medium with 4.5 µM 2,4-D. A higher rate of shoot induction was observed in RB855156 with the addition of 8.9 and 17.8 µM benzylaminopurine (BAP); this higher rate was also observed in RB72454 with the addition of 17.8 µM BAP. Murashige and Skoog (1962) (MS) medium containing 2.5 and 5.0 µM indolebutyric acid (IBA) for RB855156 and RB72454, respectively, was suitable for rooting. The plantlets were successfully acclimatized and the plant survival rate was 100% for both cultivars. Histological analysis revealed that the shoots in both cultivars originated from somatic embryogenesis Universidade Estadual de Maringá2014-01-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1634210.4025/actasciagron.v36i1.16342Acta Scientiarum. Agronomy; Vol 36 No 1 (2014); 63-72Acta Scientiarum. Agronomy; v. 36 n. 1 (2014); 63-721807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342/pdf_1Alcantara, Giovana Bomfim deDibax, RobersonBespalhok Filho, João CarlosDaros, Edelclaitoninfo:eu-repo/semantics/openAccess2014-07-24T11:30:55Zoai:periodicos.uem.br/ojs:article/16342Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2014-07-24T11:30:55Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
title |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
spellingShingle |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 Alcantara, Giovana Bomfim de tissue culture somatic embryos anatomy 2 4-D Poaceae Ciências Agrárias Fitotecnia Melhoramento Vegetal |
title_short |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
title_full |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
title_fullStr |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
title_full_unstemmed |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
title_sort |
Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342 |
author |
Alcantara, Giovana Bomfim de |
author_facet |
Alcantara, Giovana Bomfim de Dibax, Roberson Bespalhok Filho, João Carlos Daros, Edelclaiton |
author_role |
author |
author2 |
Dibax, Roberson Bespalhok Filho, João Carlos Daros, Edelclaiton |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Alcantara, Giovana Bomfim de Dibax, Roberson Bespalhok Filho, João Carlos Daros, Edelclaiton |
dc.subject.por.fl_str_mv |
tissue culture somatic embryos anatomy 2 4-D Poaceae Ciências Agrárias Fitotecnia Melhoramento Vegetal |
topic |
tissue culture somatic embryos anatomy 2 4-D Poaceae Ciências Agrárias Fitotecnia Melhoramento Vegetal |
description |
The development of an efficient regeneration system is crucial for the genetic transformation of sugarcane. The aims of this study were to regenerate plants from somatic embryos and analyze the origin and division pattern of cells during the different embryonic developmental stages of the sugarcane cultivars RB855156 and RB72454. For both cultivars, the best results for embryogenic callus induction were obtained with explant cultures on culture medium containing 13.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by callus culture on solid medium with 4.5 µM 2,4-D. A higher rate of shoot induction was observed in RB855156 with the addition of 8.9 and 17.8 µM benzylaminopurine (BAP); this higher rate was also observed in RB72454 with the addition of 17.8 µM BAP. Murashige and Skoog (1962) (MS) medium containing 2.5 and 5.0 µM indolebutyric acid (IBA) for RB855156 and RB72454, respectively, was suitable for rooting. The plantlets were successfully acclimatized and the plant survival rate was 100% for both cultivars. Histological analysis revealed that the shoots in both cultivars originated from somatic embryogenesis |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342 10.4025/actasciagron.v36i1.16342 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342 |
identifier_str_mv |
10.4025/actasciagron.v36i1.16342 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 36 No 1 (2014); 63-72 Acta Scientiarum. Agronomy; v. 36 n. 1 (2014); 63-72 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1750315306327212032 |