‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds
Main Author: | |
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Publication Date: | 2015 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Acta Scientiarum. Agronomy (Online) |
Download full: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425 |
Summary: | This study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the electrophoretic analysis of heat-resistant proteins were performed. A higher germination speed and respiratory rate was obtained for seeds with a water content of 12% w.b., and the activity of catalase isoforms was more pronounced in seeds with a higher degree of dehydration, which was in contrast to peroxidase, showed decreased activity. The seeds contained heat-resistant proteins of low molecular weight that ranged from 48.7 to 13.2 kDa. We found that the acquisition of drying tolerance in R. Elaeocarpum Reissek seeds is associated more with catalase than with esterase and peroxidase. |
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‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seedsdryinggerminationisoenzymesrespiration rateheat-resistant proteinsProdução e beneficiamento de sementesThis study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the electrophoretic analysis of heat-resistant proteins were performed. A higher germination speed and respiratory rate was obtained for seeds with a water content of 12% w.b., and the activity of catalase isoforms was more pronounced in seeds with a higher degree of dehydration, which was in contrast to peroxidase, showed decreased activity. The seeds contained heat-resistant proteins of low molecular weight that ranged from 48.7 to 13.2 kDa. We found that the acquisition of drying tolerance in R. Elaeocarpum Reissek seeds is associated more with catalase than with esterase and peroxidase. Universidade Estadual de Maringá2015-05-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa experimental; material inédito oriundo de investigação científicaapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1942510.4025/actasciagron.v37i2.19425Acta Scientiarum. Agronomy; Vol 37 No 2 (2015); 181-189Acta Scientiarum. Agronomy; v. 37 n. 2 (2015); 181-1891807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425/pdf_67Silva, Lílian Abadia daSales, Juliana de FátimaOliveira, João AlmirPinho, Édila Vilela de Resende VonSantos, Heloisa Oliveira dosSoares, Marcos Antônioinfo:eu-repo/semantics/openAccess2015-05-04T09:36:48Zoai:periodicos.uem.br/ojs:article/19425Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2015-05-04T09:36:48Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
title |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
spellingShingle |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds Silva, Lílian Abadia da drying germination isoenzymes respiration rate heat-resistant proteins Produção e beneficiamento de sementes |
title_short |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
title_full |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
title_fullStr |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
title_full_unstemmed |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
title_sort |
‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds |
author |
Silva, Lílian Abadia da |
author_facet |
Silva, Lílian Abadia da Sales, Juliana de Fátima Oliveira, João Almir Pinho, Édila Vilela de Resende Von Santos, Heloisa Oliveira dos Soares, Marcos Antônio |
author_role |
author |
author2 |
Sales, Juliana de Fátima Oliveira, João Almir Pinho, Édila Vilela de Resende Von Santos, Heloisa Oliveira dos Soares, Marcos Antônio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Lílian Abadia da Sales, Juliana de Fátima Oliveira, João Almir Pinho, Édila Vilela de Resende Von Santos, Heloisa Oliveira dos Soares, Marcos Antônio |
dc.subject.por.fl_str_mv |
drying germination isoenzymes respiration rate heat-resistant proteins Produção e beneficiamento de sementes |
topic |
drying germination isoenzymes respiration rate heat-resistant proteins Produção e beneficiamento de sementes |
description |
This study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the electrophoretic analysis of heat-resistant proteins were performed. A higher germination speed and respiratory rate was obtained for seeds with a water content of 12% w.b., and the activity of catalase isoforms was more pronounced in seeds with a higher degree of dehydration, which was in contrast to peroxidase, showed decreased activity. The seeds contained heat-resistant proteins of low molecular weight that ranged from 48.7 to 13.2 kDa. We found that the acquisition of drying tolerance in R. Elaeocarpum Reissek seeds is associated more with catalase than with esterase and peroxidase. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa experimental; material inédito oriundo de investigação científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425 10.4025/actasciagron.v37i2.19425 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425 |
identifier_str_mv |
10.4025/actasciagron.v37i2.19425 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425/pdf_67 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 37 No 2 (2015); 181-189 Acta Scientiarum. Agronomy; v. 37 n. 2 (2015); 181-189 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305909310062592 |