Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29719 |
Resumo: | Biomass from lignocellulosic material constitutes a promising energy alternative and without competing with food production. However, pretreatments are required for conversion into sugars which release hexoses, pentoses and other sugars, coupled to inhibitors. Current analysis focuses on ethanol production with the three major inhibitors of lignocellulosic biomass pretreatment, namely, acetic acid, furfural and 5-hydroxymetilfurfural (HMF), and investigates the influence of a mixture of these inhibitors on fermentation by Pichia stipits, using commercial xylose as the only carbon source, through a full factorial 23 + 3 design of experiments (DOE). Fermentations were conducted in a laboratory scale, at 150 rpm and 72h, in a complex culture media with xylose and different inhibitor concentrations, based on the experimental analysis of sugarcane bagasse and 2.107 cell mL-1 of initial concentration of the microorganism. Experimental results showed a significant influence of acetic acid concentration, which must be at the lowest possible level, with no influence of furfural and hydroxymethyl furfural respectively up to concentrations 2.25 and 0.75 g L-1. |
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Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture mediabiomassxyloseethanolPichia stipitisinhibitors.Engenharia QuímicaBiomass from lignocellulosic material constitutes a promising energy alternative and without competing with food production. However, pretreatments are required for conversion into sugars which release hexoses, pentoses and other sugars, coupled to inhibitors. Current analysis focuses on ethanol production with the three major inhibitors of lignocellulosic biomass pretreatment, namely, acetic acid, furfural and 5-hydroxymetilfurfural (HMF), and investigates the influence of a mixture of these inhibitors on fermentation by Pichia stipits, using commercial xylose as the only carbon source, through a full factorial 23 + 3 design of experiments (DOE). Fermentations were conducted in a laboratory scale, at 150 rpm and 72h, in a complex culture media with xylose and different inhibitor concentrations, based on the experimental analysis of sugarcane bagasse and 2.107 cell mL-1 of initial concentration of the microorganism. Experimental results showed a significant influence of acetic acid concentration, which must be at the lowest possible level, with no influence of furfural and hydroxymethyl furfural respectively up to concentrations 2.25 and 0.75 g L-1. Universidade Estadual De Maringá2017-05-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionprocesso fermentativoapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/2971910.4025/actascitechnol.v39i2.29719Acta Scientiarum. Technology; Vol 39 No 2 (2017); 223-229Acta Scientiarum. Technology; v. 39 n. 2 (2017); 223-2291806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29719/pdfCopyright (c) 2017 Acta Scientiarum. Technologyinfo:eu-repo/semantics/openAccessAbud, Ana Karla de SouzaOliveira Junior, Antonio Martins deSilva, Augusto de Souza da2017-05-15T17:12:51Zoai:periodicos.uem.br/ojs:article/29719Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2017-05-15T17:12:51Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
title |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
spellingShingle |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media Abud, Ana Karla de Souza biomass xylose ethanol Pichia stipitis inhibitors. Engenharia Química |
title_short |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
title_full |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
title_fullStr |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
title_full_unstemmed |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
title_sort |
Effect of inhibitors on ethanol production by Pichia stipitis in a complex culture media |
author |
Abud, Ana Karla de Souza |
author_facet |
Abud, Ana Karla de Souza Oliveira Junior, Antonio Martins de Silva, Augusto de Souza da |
author_role |
author |
author2 |
Oliveira Junior, Antonio Martins de Silva, Augusto de Souza da |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Abud, Ana Karla de Souza Oliveira Junior, Antonio Martins de Silva, Augusto de Souza da |
dc.subject.por.fl_str_mv |
biomass xylose ethanol Pichia stipitis inhibitors. Engenharia Química |
topic |
biomass xylose ethanol Pichia stipitis inhibitors. Engenharia Química |
description |
Biomass from lignocellulosic material constitutes a promising energy alternative and without competing with food production. However, pretreatments are required for conversion into sugars which release hexoses, pentoses and other sugars, coupled to inhibitors. Current analysis focuses on ethanol production with the three major inhibitors of lignocellulosic biomass pretreatment, namely, acetic acid, furfural and 5-hydroxymetilfurfural (HMF), and investigates the influence of a mixture of these inhibitors on fermentation by Pichia stipits, using commercial xylose as the only carbon source, through a full factorial 23 + 3 design of experiments (DOE). Fermentations were conducted in a laboratory scale, at 150 rpm and 72h, in a complex culture media with xylose and different inhibitor concentrations, based on the experimental analysis of sugarcane bagasse and 2.107 cell mL-1 of initial concentration of the microorganism. Experimental results showed a significant influence of acetic acid concentration, which must be at the lowest possible level, with no influence of furfural and hydroxymethyl furfural respectively up to concentrations 2.25 and 0.75 g L-1. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion processo fermentativo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29719 10.4025/actascitechnol.v39i2.29719 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29719 |
identifier_str_mv |
10.4025/actascitechnol.v39i2.29719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29719/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Acta Scientiarum. Technology info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Acta Scientiarum. Technology |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 39 No 2 (2017); 223-229 Acta Scientiarum. Technology; v. 39 n. 2 (2017); 223-229 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315336295612416 |